A method of processing miniature organs

Abstract

The invention provides a treatment method of a micro organ. The treatment method comprises the following step sequentially dialyzing a fixed micro organ in dialysate I for 2 to 5 days under 1 to 10 DEG C and in dialysate II for 4 to 8 hours under -40 to -15 DEG C, wherein a formula of the dialysate I comprises 1M of Tris alkaline solution which is prepared from 10 percent of Triton-X 100, 2 percent of BSA (Bull Serum Albumin) and 0.05M of NaCl, and the pH (Potential of Hydrogen) is 6.8; a formula of the dialysate II comprises ethanol and DMSO (Dimethyl Sulfoxide) which are 4 to 1 in volume ratio. The treatment method provided by the invention is used for carrying out sizing and whole permeation treatment on the micro organ, an original morphology of the micro organ is kept, and the purposeof enabling macromolecular matters to fully permeate each position without changing the structural morphology of the micro organ and tissue cells can be realized; the treated micro organ is capable of accurately and sensitively carrying out cellular or subcellular location and qualitative and relatively quantitative analysis on molecules on the micro organ by utilizing specific binding reaction of molecular matters and through corresponding observation means such as a fluorescent microscope. Aiming at the difficulties that internal organs of animalcules in the field of biology are very smalland a tissue section technique is difficult to implement for the internal organs, the method for researching in-vivo micro organ on molecular and cellular level is invented.

Description

technical field [0001] The invention relates to a method for treating tissues and organs, in particular to tiny tissues and organs in animals. [0002] technical background [0003] The animal body is a combination of various tissues and organs, and the life activities of animals must be maintained by the normal performance of their respective physiological and biological functions by each tissue and organ. The study of tissues and organs is the premise and important link for in-depth understanding of animals and the control and utilization of animals. [0004] Histochemistry, a commonly used tissue and organ pathological detection method in medical diagnosis, has been sublimated into immunohistochemistry in the biological field with the development of molecular and cell biology. Immunohistochemistry skillfully combines the immunoreaction specificity of antigen-antibody binding with the visibility of histochemistry, detects antigenic substances at the cellular and subcellula...

AI Technical Summary

Problems solved by technology

Usually, the organs of tiny animals are very small, and the original tissue anatomy research conditions are demanding and difficult, especially some with a thickness of less than 1mm, which are difficult to observe with the naked eye, and the outermost layer is non-keratinized or ossified. Internal micro-organs whose reflection contrast is very small and whose visibility is close to 0 (for example, unlike external tissues and organs such as the body wall and head of silkworm larvae, in order to resist damage to internal tissues by sharp objects, the outermost layer is keratinized and ossified , after transparent treatment, it still retains a certain light reflection contrast and visibility), when scientific research is carried out at the molecular level, because there is no suitable and feasible method, it seriously hinders related research and becomes a bottleneck for in-depth research.

Images