A prefabricated reagent for rapid detection of hydroxymethylfurfural in honey and its storage and detection methods

A technology of hydroxymethylfurfural and hydroxymethylfurfural oxidase, which is applied in the direction of material analysis by observing the impact on chemical indicators, and analysis by making materials undergo chemical reactions, can solve the problem of large errors and hydroxymethylfurfural Low detection efficiency and other problems, to achieve the effect of high consistency, intuitive and visible results, and simple operation process

Active Publication Date: 2020-06-05
HANGZHOU TIANMAI BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the problems of low detection efficiency and large error of hydroxymethylfurfural in traditional honey, the present invention provides a prefabricated reagent for rapid detection of hydroxymethylfurfural in honey with high sensitivity and strong anti-interference ability

Method used

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  • A prefabricated reagent for rapid detection of hydroxymethylfurfural in honey and its storage and detection methods
  • A prefabricated reagent for rapid detection of hydroxymethylfurfural in honey and its storage and detection methods

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Effect test

Embodiment 1

[0032] A prefabricated reagent for rapid detection of hydroxymethylfurfural in honey, consisting of the following raw materials: 9.0g of sodium chloride, 0.1g of N,N-diethyl-p-phenylenediamine, 0.7g of phosphate (disodium hydrogen phosphate and The molar ratio of sodium dihydrogen phosphate is 1:5), hydroxymethylfurfural oxidase 0.1g and horseradish peroxidase 0.1g; Based on 10g of the prefabricated reagent total mass of hydroxymethylfurfural rapid detection in honey, the The enzyme activity unit containing hydroxymethylfurfural oxidase in the prefabricated reagent for rapid detection of hydroxymethylfurfural is 1.2U / mg, and the enzyme activity unit containing horseradish peroxidase is 2.5U / mg; the hydroxymethylfurfural in the above honey The prefabricated reagents for the rapid detection of furfural are divided into 50mg portions, and each portion is sealed and packaged in a 10mL transparent screw-cap glass test tube, and refrigerated at 4°C in the dark.

Embodiment 2

[0034] A prefabricated reagent for rapid detection of hydroxymethylfurfural in honey, consisting of the following raw materials: 8.5g of sodium chloride, 0.05g of N,N-diethyl-p-phenylenediamine, 0.6g of phosphate (disodium hydrogen phosphate and The molar ratio of sodium dihydrogen phosphate 12:1), hydroxymethylfurfural oxidase 0.45g and horseradish peroxidase 0.4g; Based on the prefabricated reagent total mass 10g of hydroxymethylfurfural rapid detection in honey, the The enzyme activity unit containing HMF oxidase in the prefabricated reagent for rapid detection of HMF is 1.5U / mg, and the enzyme activity unit containing horseradish peroxidase is 3.0U / mg; The prefabricated reagents for rapid detection of furfural are divided into 50mg portions, each portion is sealed and packaged in a 10mL high-transparency polystyrene plastic bottle, and refrigerated at 8°C in the dark.

Embodiment 3

[0036]A prefabricated reagent for rapid detection of hydroxymethylfurfural in honey, consisting of the following raw materials: 9.5g of sodium chloride, 0.15g of N,N-diethyl-p-phenylenediamine, 0.25g of phosphate (disodium hydrogen phosphate and The molar ratio of sodium dihydrogen phosphate 1:0.5), hydroxymethylfurfural oxidase 0.05g and horseradish peroxidase 0.05g; Based on the prefabricated reagent total mass 10g of the rapid detection of hydroxymethylfurfural in honey, the The prefabricated reagent for the rapid detection of hydroxymethylfurfural contains 0.5 U / mg of enzyme activity unit of hydroxymethylfurfural oxidase, and the enzyme activity unit of horseradish peroxidase is 1.0 U / mg; the hydroxymethylfurfural in the above honey The prefabricated reagents for the rapid detection of furfural are divided into 50mg portions, each portion is sealed and packaged in a 10mL transparent screw-cap glass test tube, and refrigerated at 6°C in the dark.

[0037] To the prefabricat...

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Abstract

The invention provides a prepared reagent for quickly detecting hydroxymethyl furfural in honey and a storage method and a detection method of the prepared reagent. The prepared reagent is prepared from the following raw materials in parts by weight: 85-95 parts of a filler, 0.5-1.5 parts of a color developing agent, 2.5-7 parts of phosphate, 0.5-4.5 parts of hydroxymethyl furfural oxidase and 0.5-4 parts of horse radish peroxidase. The operation is simple, the prepared reagent is safe and convenient to carry, the operation process is simple and efficient, results are visual and visible, colordevelopment is in a visible light area, and color changes can be seen by naked eyes; the detection sensitivity is high, and by use of a spectrophotometer, the limit of detection can reach 0.15 mg / L;the detection method is high in recovery rate and excellent in reproducibility; the detection results are high in conformity with that obtained by the national standard high-performance liquid chromatography; the average recovery rate reaches 98.5%, and the relative standard deviation (n=10) is 3.86%; and the detection recovery rate is high, and the reproducibility is good.

Description

technical field [0001] The invention relates to the technical field of rapid detection of food safety, in particular to a prefabricated reagent for the rapid detection of hydroxymethylfurfural in honey with high sensitivity and simple operation, and a preservation and detection method thereof. Background technique [0002] The natural sweet substance that the bee collects the nectar, secretion or honeydew of the plant, mixes it with its own secretion, and fully brews it. Honey contains a variety of inorganic salts and vitamins, iron, calcium, copper, manganese, potassium, phosphorus and other organic acids and trace elements that are beneficial to human health. It also contains sugars and a variety of amino acids. High health effects and nutritional value, deeply favored by consumers. In recent years, the demand for honey in the domestic and international markets has continued to expand. However, the output of honey is difficult to meet the demand of the market, and due to ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
Inventor 赵澎涛黄光荣吴东兵唐玉龙陈维强
Owner HANGZHOU TIANMAI BIO TECH
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