Preparation method of fish meal-free soybean protein peptide feed for neritic fish
A soybean protein peptide and fish meal-free technology, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, and animal feed molding or processing, can solve the problems of high fish meal prices, declining breeding profits, and increasing breeding costs. , to achieve the effect of protecting water quality and ecological environment, water pollution is small, and the proportion is moderate
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Embodiment 1
[0012] The preparation method of fishmeal-free shallow sea fish soybean protein peptide feed includes activation culture, soybean meal solid-state fermentation, preparation of feed, and spraying. The specific operation steps are as follows:
[0013] 1) Activation culture: Bacillus natto was inoculated on the slant medium by aseptic operation, and cultured at 31°C for 2.4 days; Under the condition of shaking at 175r / min for 4 hours, the components and parts by weight of the slant medium are: 0.18 parts of beef extract, 0.46 parts of peptone, 0.5 parts of sodium chloride, 2.4 parts of agar, 98 parts of water, the components of the liquid activated medium and Its parts by weight are: 1 part of beef extract, 3 parts of peptone, 3 parts of sodium chloride, and 280 parts of water. The Bacillus natto used was purchased from China Agricultural Microorganism Culture Collection Center;
[0014] 2) Soybean meal solid-state fermentation: Inoculate the Bacillus natto bacterial liquid obtai...
Embodiment 2
[0018] The preparation method of fishmeal-free shallow sea fish soybean protein peptide feed includes activation culture, soybean meal solid-state fermentation, preparation of feed, and spraying. The specific operation steps are as follows:
[0019] 1) Activation culture: Bacillus natto was inoculated on the slant medium by aseptic operation, and cultured at 31°C for 2.4 days; Under the condition of 176r / min, shaking culture for 5h, the components and parts by weight of the slant medium are: 0.14 parts of beef extract, 0.5 parts of peptone, 0.6 parts of sodium chloride, 2 parts of agar, 99 parts of water, the components of the liquid activated medium and Its parts by weight are: 2 parts of beef extract, 4 parts of peptone, 4 parts of sodium chloride, and 300 parts of water. The Bacillus natto used was purchased from China Agricultural Microorganism Culture Collection Center;
[0020] 2) Soybean meal solid-state fermentation: Inoculate the Bacillus natto bacterial liquid obtain...
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