Preparation method of amino acid water soluble fertilizer
A water-soluble fertilizer and amino acid technology, applied in the direction of microbial-based methods, biochemical equipment and methods, fertilizer mixtures, etc., can solve the problems of high-purity amino acid prices and increased fertilizer costs, so as to improve hydrolysis rate and aerobic Fermentation rate, effect of accelerating enzymatic hydrolysis
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Embodiment 1
[0022] A preparation method of amino acid water-soluble fertilizer includes the following steps:
[0023] (1) Acid-base hydrolysis: add acetic acid to the culture solution in an amount of 3.5% of the quality of the culture solution, and let it stand for 2.5 hours at a constant temperature of 28°C; then, add ammonia water to the culture solution in an amount of 4.5% of the culture solution's quality to adjust pH7.5-8, and stand for 1.5h at a constant temperature of 29℃;
[0024] (2) Enzymatic hydrolysis reaction: add mixed enzymes to the culture solution after acid-base hydrolysis, the added amount is 1.5% of the culture solution mass, after uniform mixing, stir 44min in a 31℃ thermostat to prepare enzymatic hydrolysis culture liquid;
[0025] (3) Fermentation of culture broth: Add mixed strains to the enzymolysis culture broth according to 3.5% of the mass of the enzymatic culture broth, mix them evenly, and place them in a fermentor for fermentation process;
[0026] The parameters ...
Embodiment 2
[0036] Comparing this embodiment 2 with embodiment 1, the steps change in the following aspects:
[0037] Step (1) Acid-base hydrolysis, in which the amount of acetic acid added is 4% of the mass of the culture solution, the acidolysis temperature is 30°C, and the time is 3h; the amount of ammonia added is 5%, the alkaline hydrolysis temperature is 31°C, and the time is 2h.
[0038] Step (2) Enzymatic hydrolysis reaction, wherein the added amount of mixed enzyme is 2% of the mass of the culture solution, enzymatic hydrolysis is performed at 33° C., and stirring is performed for 47 minutes.
[0039] Step (3) Fermentation of culture broth, the fermentation parameters are:
[0040] Carry out aerobic fermentation first, the temperature rise of the fermenter is 5℃ / 30min, the temperature reaches 37℃, cultivate for 3h, the dissolved oxygen in the culture solution is 6L / min; then carry out anaerobic fermentation, stop the dissolved oxygen, and ferment The tank was evacuated to a vacuum, the f...
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