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Method for extracting purple sweet potato anthocyanin and application

A technology of anthocyanins and purple sweet potatoes, which is applied in chemical instruments and methods, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve the problems of difficult recovery, high cost, health hazards, etc., to improve extraction efficiency and increase yield efficiency and cost savings

Inactive Publication Date: 2018-01-19
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The traditional solvent extraction method uses ethanol as the extractant, which has high efficiency, but the relative cost is high, recovery is difficult, and it will bring a series of by-products, which are potential health hazards

Method used

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  • Method for extracting purple sweet potato anthocyanin and application
  • Method for extracting purple sweet potato anthocyanin and application
  • Method for extracting purple sweet potato anthocyanin and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A polyethylene glycol extraction method utilizing response surface methodology to optimize purple sweet potato anthocyanins comprises the following steps:

[0047] (1) Sample preparation:

[0048]Wash fresh purple sweet potatoes, cut them into thin slices with a thickness of about 0.3-0.6 cm on a chopping board, dry them at 60°C, crush them, and pass through a 100-mesh sieve. Accurately weigh 5g of purple sweet potato powder sample in a 500mL conical flask, add a polyethylene glycol solution with a volume concentration of 65-95% at a liquid-to-solid ratio of 25-55mL / g, and extract 65% by ultrasonic in a constant temperature water bath at 45-75°C. -95min, cooling. Centrifuge at 11,000 rpm for 10 min, collect the supernatant, and obtain extracts corresponding to different extraction conditions.

[0049] (2) Determination of total anthocyanin content in the extract by pH differential method

[0050] ① Preparation of buffer solution:

[0051] pH1.0 buffer solution: accu...

Embodiment 2

[0078] Wash fresh purple sweet potatoes, cut them into thin slices with a thickness of about 0.3-0.6 cm on a chopping board, dry them at 60°C, crush them, and pass through a 100-mesh sieve.

[0079] Accurately weigh 5g of purple sweet potato powder sample into a 500mL Erlenmeyer flask, add polyethylene glycol solution with a volume percentage concentration of 91.55% at a liquid-to-solid ratio of 44.05mL / g, place in a constant temperature water bath at 69.3°C, ultrasonically extract for 80min, and cool. Centrifuge at 11000 rpm for 10 min, collect the supernatant, and obtain purple sweet potato anthocyanin extract.

[0080] The total anthocyanin content in the extract was determined by the pH differential method, and the actual anthocyanin yield was 78.01mg / 100g, which was close to the predicted value, indicating that the optimized extraction conditions were reliable.

Embodiment 3

[0082] Wash fresh purple sweet potatoes, cut them into thin slices with a thickness of about 0.3-0.6 cm on a chopping board, dry them at 60°C, crush them, and pass through a 100-mesh sieve.

[0083] Accurately weigh 5g of purple sweet potato powder sample into a 500mL Erlenmeyer flask, add polyethylene glycol solution with a volume percentage concentration of 90% at a liquid-to-solid ratio of 40mL / g, place in a constant temperature water bath at 65°C, ultrasonically extract for 75min, and cool. Centrifuge at 11000 rpm for 10 min, collect the supernatant, and obtain purple sweet potato anthocyanin extract.

[0084] The total anthocyanin content in the extract was determined by pH differential method, and the actual yield of anthocyanin was 76.54mg / 100g.

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Abstract

The invention discloses a method for extracting a purple sweet potato anthocyanin and an application, and belongs to the technical field of plant anthocyanin extraction. The method comprises the following steps: using purple sweet potato powder as a raw material, adding a polyethylene glycol solution with a volume concentration of 90-95% according to a liquid-material ratio of 40-50 mL / g, performing ultrasonic extraction for 75-85 minutes in a water bath of 65-75 DEG C, performing cooling, and separating and collecting a supernatant to obtain a purple sweet potato anthocyanin extraction liquid. According to the invention, extraction of the purple sweet potato anthocyanin is performed by utilizing a combination of polyethylene glycol and an ultrasonic technology, reduction of by-products isfacilitated, costs are saved, potential safety hazards caused by using toxic reagents are avoided, and the polyethylene glycol has the effects of protecting the anthocyanin and improving extraction stability; and by utilizing the method provided by the invention, the extraction time is shortened, and meanwhile the yield of the anthocyanin is improved. The invention has simple operation, saves costs, improves production efficiency, promotes food safety, and has better economic and social benefits.

Description

technical field [0001] The invention relates to the technical field of extracting plant anthocyanins, in particular to a method and application for extracting purple potato anthocyanins. Background technique [0002] Anthocyanin is the main coloring substance widely present in the flowers, fruits, seeds and leaves of plants in nature. It is a water-soluble natural pigment and belongs to flavonoids. Plant anthocyanins have a variety of physiological activities and pharmacological effects, such as antioxidant activity, antitumor, antimutagenicity, liver protection, antihyperglycemia, etc., and have broad application prospects. [0003] Purple sweet potato has a wide range of planting in my country, low cost of raw materials, weak seasonality, and its pulp and peel contain a large amount of brightly colored natural anthocyanins. Purple potato anthocyanins are rich in acylated structures, which makes it more stable than other natural pigments. Studies have shown that purple sw...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/065C07H1/08A61K31/7048A61P39/06A61P35/00A61P1/16A61P3/10A23L33/105A23L5/43A61K8/60A61Q19/00
Inventor 罗自生黄皓李莉
Owner ZHEJIANG UNIV
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