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Technology for producing lycopene by biological fermentation

A technology of lycopene and biological fermentation, applied in the direction of microorganism-based methods, fermentation, microorganisms, etc., can solve the problems of high production cost, inability to accumulate lycopene, low fermentation yield, etc., and achieve the effect of facilitating absorption

Inactive Publication Date: 2018-01-19
宋宏婷
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of using microbial fermentation to produce lycopene is that microorganisms cannot accumulate lycopene at a high level, resulting in low fermentation yield and high production costs.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] This embodiment provides a process for producing lycopene by biological fermentation, which includes the following steps:

[0038] Step 1: Take 200g of peeled potatoes, add 1000mL of deionized water to boil and keep for 30min; after cooling, filter through two layers of gauze, take the filtrate, add deionized water to 1000mL, add 20g of glucose and 10g of agar; sterilize at 115°C After 30 minutes, potato culture medium (PDA) was prepared. Take ATCC 14271 (+) (hereinafter referred to as positive bacteria) and ATCC 14272 (-) (hereinafter referred to as negative bacteria) and spread them on PDA plates respectively, culture them in a constant temperature incubator at 26°C for 4 days, then lower the temperature to 16°C, Stimulate sporulation, and feed oxygen into the incubator at a rate of 2 L / min, and at the same time give light with an intensity of 4WLx to cultivate spores for 3 days.

[0039] Step 2: Connect positive bacteria spores and negative bacteria spores respectiv...

Embodiment 2

[0044] This embodiment provides a process for producing lycopene by biological fermentation, which includes the following steps:

[0045] Step 1: Take 200g of peeled potatoes, add 1000mL of deionized water to boil and keep for 30min; after cooling, filter through two layers of gauze, take the filtrate, add deionized water to 1000mL, add 20g of glucose and 10g of agar; sterilize at 115°C After 30 minutes, potato medium (PDA) was prepared. Take ATCC 14271 (+) (hereinafter referred to as positive bacteria) and ATCC 14272 (-) (hereinafter referred to as negative bacteria) and spread them on PDA plates respectively, culture them in a constant temperature incubator at 28°C for 3 days, then lower the temperature to 18°C, Stimulate spore formation, and feed oxygen into the incubator at a rate of 2 L / min, and at the same time give light with an intensity of 4WLx to cultivate spores for 2 days.

[0046] Step 2: Connect positive bacteria spores and negative bacteria spores respectively ...

Embodiment 3

[0051] This embodiment provides a process for producing lycopene by biological fermentation, which includes the following steps:

[0052] Step 1: Take 200g of peeled potatoes, add 1000mL of deionized water to boil and keep for 30min; after cooling, filter through two layers of gauze, take the filtrate, add deionized water to 1000mL, add 20g of glucose and 10g of agar; sterilize at 115°C After 30 minutes, potato medium (PDA) was prepared. Take ATCC 14271 (+) (hereinafter referred to as positive bacteria) and ATCC 14272 (-) (hereinafter referred to as negative bacteria) and spread them on PDA plates, culture them in a constant temperature incubator at 30°C for 2 days, then lower the temperature to 20°C, Stimulate spore formation, and feed oxygen into the incubator at a rate of 2L / min, and at the same time give light with an intensity of 4WLx to cultivate spores for 1d.

[0053] Step 2: Connect positive bacteria spores and negative bacteria spores respectively into a triangular ...

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PUM

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Abstract

The invention discloses a technology for producing lycopene by biological fermentation and relates to the field of biological pharmacy. The technology for producing the lycopene by the biological fermentation, disclosed by the invention, comprises the following steps: inoculating positive fungus spores and negative fungus spores into saline solutions respectively to obtain a positive fungus sporesuspension solution and a negative fungus spore suspension solution; inoculating the positive fungus spore suspension solution and the negative fungus spore suspension solution into a seed culture medium according to the concentration ratio of 2 to (3 to 5) to obtain a seed solution; inoculating the seed solution into a fermentation culture medium and carrying out fermentation culture; after carrying out the fermentation culture for 12h, supplementing a fermentation culture medium which is equivalent to an original fermentation culture medium into a fermented solution. By adopting the technology disclosed by the invention, a form of the seed solution is improved; the positive spore suspension solution and the negative spore suspension solution are directly inoculated into the seed culturemedium to carry out conjugation; produced thalli are extremely small fungus pellets or thalli and pelletized thalli are not generated, so that the absorption of oxygen and nutrient substances in a liquid culture medium is facilitated; fermentation conditions are improved so that the metabolism of the thalli and the expression activity of a product are enhanced; the accumulation of the lycopene isfacilitated and the yield of the lycopene is improved.

Description

technical field [0001] The invention relates to the field of biopharmaceuticals, in particular to a process for producing lycopene by biological fermentation. Background technique [0002] Lycopene is a carotenoid that has the functions of improving human immunity, anti-cancer, anti-oxidation, lowering blood fat, and protecting the skin. It is widely used in the fields of food, medicine, and cosmetics. Natural lycopene mainly exists in the ripe fruit of tomato, a plant in the family Solanaceae. As people pay more and more attention to green and health, lycopene has been widely concerned by people. Humans cannot synthesize lycopene by themselves, so they need to be supplemented through diet and other means. According to the forecast of CMR (Customer Management Relationship) International Company in the United States, the sales of lycopene products will increase by 35% annually, and the sales volume is expected to reach 20 billion US dollars in two or three years. The world'...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P5/02C12R1/645
Inventor 宋宏婷
Owner 宋宏婷
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