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Method for detecting nitrofuran drug in eel

A technology of nitrofuran and detection method, which can be applied to measurement devices, instruments, scientific instruments and other directions, can solve problems such as mutagenesis and carcinogenesis, and achieve the effects of high precision and accuracy, high extraction efficiency, and simple pretreatment method.

Inactive Publication Date: 2018-01-19
CHANGLE JUQUAN FOOD
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Despite their great therapeutic value, nitrofurans and their metabolites are mutagenic and carcinogenic

Method used

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  • Method for detecting nitrofuran drug in eel
  • Method for detecting nitrofuran drug in eel
  • Method for detecting nitrofuran drug in eel

Examples

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Embodiment Construction

[0025] 1. Solution preparation:

[0026] Standard stock solution: Weigh 20 mg each of AOZ, AMOZ, AHD, and SEM, and dilute to a 100 mL brown volumetric flask with methanol to make a 200 mg / L standard stock solution, and store in a -18°C freezer away from light;

[0027] Mixed standard stock solution: Dilute the standard stock solution with methanol to prepare a mixed standard solution of 1.0 mg / L for AOZ, AMOZ, AHD, and SEM, and store it in a -18°C freezer away from light;

[0028] Mixed standard working solution: dilute the mixed standard solution with methanol, prepare a mixed standard solution of 40 μg / L for AOZ, AMOZ, AHD, and SEM, and store it in a 5°C refrigerator away from light;

[0029] Preparation of derivatizer: weigh 0.2g of o-nitrobenzaldehyde and dissolve it in 20mL of methanol, add 5mL of glacial acetic acid, shake well, and set aside;

[0030] The distilled water required for other solutions in the experiment was ultrapure water purified by the Milli-Q ultrapur...

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Abstract

The invention relates to a method for detecting a nitrofuran drug in eel. The method comprises the following steps: weighing 4 g of an eel sample, adding 20 ml of a hydrochloric acid solution with concentration being 0.2 mol / L, adding 1 ml of a derivating agent, uniformly shaking with hands, performing a water-bath reaction at the temperature of 60 DEG C for 1 h, taking the sample out and coolingthe sample to room temperature, using a NaOH solution for adjusting the pH value to 7.0-7.5, performing centrifugation for 10 min, taking a half of a supernatant, using 5 ml of methanol and 5 ml of distilled water, activating a solid-phase extraction column; separating the supernatant by a column, using 5 ml of distilled water for leaching, removing a leacheate, using 2 ml of methanol for elutinga solid-phase extraction column, collecting an eluate, drying the material with nitrogen at the temperature of 40 DEG C, using 1 ml of a furan solution for dissolving, filtering the material by a microfiltration membrane, and performing HPLC-MS-MS detection. The method has the advantages of high extraction efficiency, short derivating time, and high sensitivity, precision and accuracy.

Description

technical field [0001] The invention specifically relates to a method for detecting nitrofuran drugs in eels. Background technique [0002] Nitrofuran drugs are broad-spectrum antibiotics, which can effectively treat human and animal diseases infected by bacteria and protozoa. Because of their low price and good curative effect, they are widely used in livestock, poultry and aquaculture. In aquaculture, it is used as a growth promoter and for the treatment and prevention of gastroenteritis caused by bacterial and protozoan infections. In poultry farming, nitrofuran drugs are mainly used to treat typhoid fever, paratyphoid fever, pullorum, mastitis, and urinary tract infections in poultry. The chemical structural formulas of nitrofuran drugs and their metabolites are as follows: [0003] [0004] [0005] Despite their great therapeutic value, nitrofurans and their metabolites are mutagenic and carcinogenic. The most common nitrofurans are furazolidone, furaltadone, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/08G01N30/14
Inventor 王家恩翁齐彪邱宗清魏心星周晓峰
Owner CHANGLE JUQUAN FOOD
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