Biomimetic cartilage material having biological activity and preparation method thereof

A technology of biological activity and biomaterials, applied in medical science, prostheses, etc., can solve problems such as insufficient mechanical strength, difficulty in control, cluster phenomenon, etc., achieve high tissue-inducing activity, good biological crystal form, and simple and efficient preparation process Effect

Inactive Publication Date: 2018-01-26
BEIJING DATSING BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mechanical strength of PVA hydrogel is insufficient, the surface is smooth, and there is no biological activity. The bonding performance between the cartilage prosthesis and the bone base is very poor, which directly affects the fixation and repair function of the cartilage prosthesis. Generally, biomaterials with osteoinductive activity such as Hydroxyapatite, bioactive glass, etc. are compounded with polyvinyl alcohol hydrogel, so the compounding of polyvinyl alcohol and inorganic materials has become the focus and hotspot of research
[0007] Most of the composite technologies reported in the literature or disclosed in the patents use simple mechanical stirring or in-situ precipitation composite technology. The main disadvantages of this process are: 1) the stirring is not u

Method used

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  • Biomimetic cartilage material having biological activity and preparation method thereof
  • Biomimetic cartilage material having biological activity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Add 20.0g of polyvinyl alcohol particles with a degree of polymerization n=1750±50 into 100mL of deionized water, stir and heat at 95°C for 6h to prepare a 20wt% polyvinyl alcohol aqueous solution, lower the temperature to 60°C, heat the solution to 98°C and let it stand After 2 hours of defoaming, pour the solution into a mold, put it into a refrigerator to freeze and shape it at -20°C for 16 hours, then take out the sample and place it at room temperature for 6 hours to thaw. Repeat the freezing and thawing process 5 times to obtain a physically cross-linked PVA hydrogel with 5 freezing and thawing times.

Embodiment 2

[0038] Add 20.0g of polyvinyl alcohol particles with a degree of polymerization n=1750±50 into 100mL of deionized water, stir and heat at 95°C for 6h to prepare a 20wt% polyvinyl alcohol aqueous solution, lower the temperature to 60°C, and add 2wt% of nano-hydroxyapatite Stone (purchased from Sigma-Aldrich Sigma-Aldrich (Shanghai) Trading Co., Ltd.), stirred overnight to make the reaction fully, and finally obtain the n-HA / PVA solution with a PVA matrix concentration of 20wt% and containing hydroxyapatite. After standing still for 2-8 hours for degassing, pour the solution into the mold, put it into the refrigerator to freeze and shape, the freezing temperature is -20°C, and the time is 16 hours, then take out the sample, and let it stand at room temperature for 6 hours to thaw. The above freezing and thawing process was repeated 5 times to prepare n-HA / PVA hydrogels with different concentrations of hydroxyapatite with 5 freezing and thawing times.

Embodiment 3

[0040] Add 20.0g of polyvinyl alcohol particles with polyvinyl alcohol n=1750±50 into 100mL of deionized water, stir and heat at 95°C for 6h to prepare a 20wt% polyvinyl alcohol aqueous solution, lower the temperature to 60°C, and add an appropriate amount of Ca (OH) 2 The powder was continuously stirred for 2 hours to prepare a uniform dispersion, and 2wt% of hydroxyapatite was added, and then the corresponding amount of H 3 PO 4 The ethanol solution (0.5mol / L) was stirred overnight to make the reaction fully, and finally the n-HA / PVA solution containing hydroxyapatite with a PVA matrix concentration of 20wt% was obtained. After the solution was left to stand for 2h to 8 hours to defoam, pour the solution into a mold, put it into the refrigerator to freeze and shape, the freezing temperature was -20°C, and the time was 16h. Then the sample was taken out and left at room temperature for 6h to thaw. The above freeze-thaw process was repeated 5 times to obtain a physically cro...

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PUM

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Abstract

The invention provides a novel biomimetic cartilage material having biological activity and a preparation method thereof. The biomimetic cartilage material is a composite material which is prepared with a biomaterial with bone induced activity and polyvinyl alcohol as raw materials and through combination of an ultrasound method and a sol-gel method. From the perspectives of material biomimetics and functional biomimetics, the improved sol-gel method (namely ultrasonic-sol-gel method) is adopted for compositing of polyvinyl alcohol and an inorganic material, a biomimetic artificial cartilage having biological induced activity is prepared, mainly manifests in relatively good integration of the material and bones and has no obvious rejection, the inorganic material has good dispersion, no agglomeration phenomenon exists, the particle size distribution is controllable, and a crystal form structure is relatively ideal.

Description

technical field [0001] The invention relates to the fields of medical equipment and biomaterials, in particular to a bionic cartilage material with bioactivity and a preparation method thereof. Background technique [0002] Joints are functional connections between bones in the skeletal system. In the synovial joint, the bone end of the joint is covered with a layer of 1-7mm thick white transparent connective tissue—articular cartilage, which is the load-bearing tissue that makes up the articular surface of the synovial joint, which can reduce the friction of the articular surface during repeated joint movements. It has the characteristics of lubrication and wear resistance, and also has the function of reducing impact and absorbing shock. [0003] Degeneration or damage of articular cartilage caused by trauma or disease is relatively common in clinical practice. However, due to the special articular cartilage tissue, there are no blood vessels, lymphatic vessels and nerves...

Claims

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Application Information

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IPC IPC(8): A61L27/46A61L27/44A61L27/50A61L27/54
Inventor 孙乐青翟晖张玉兰李湘杰李次会
Owner BEIJING DATSING BIO TECH
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