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A microfluidic chip with three-dimensional graphene interface and its preparation method

A microfluidic chip, graphene technology, applied in biochemical equipment and methods, enzymology/microbiology devices, tissue cell/virus culture devices, etc. Small contact area, unfavorable cell biological characteristics, etc., to achieve good application prospects, good biocompatibility, and controllable process parameters.

Active Publication Date: 2020-07-14
TONGXIANG BEITE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the sensing electrodes of most microfluidic chips are the classic flat two-dimensional gold electrode structure. The contact area between the electrode and the cells is small, the cell signal obtained is weak, and it cannot respond to the micro-movement of the cells in the vertical direction. and migration, so it is not conducive to the study of cell biology, especially single cells

Method used

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  • A microfluidic chip with three-dimensional graphene interface and its preparation method
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  • A microfluidic chip with three-dimensional graphene interface and its preparation method

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Embodiment Construction

[0040] The object of the present invention is to prepare a microfluidic chip with a three-dimensional graphene interface, the microfluidic chip includes two liquid storage tanks and a test flow channel between the two liquid storage tanks to form an H-shaped PDMS flow channel, And the daughter chip arranged in the test channel 21, such as figure 1 As shown, can also include openable PDMS cover ( figure 1 shaded part); planar structure parts such as figure 2 with 3 As shown, the H-shaped PDMS flow channel can prevent the clogging of the microfluidic chip when the cells are injected. The chiplet includes a central reference electrode 20 and one or more mutually independent cell electrodes symmetrically arranged on both sides of the central reference electrode 20 .

[0041] The chiplet adopts as Figure 4 The cell electrode 10 shown includes an electrode base 11 and a capture groove 12 located on the electrode base 11. The capture groove 12 is composed of a plurality of micr...

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Abstract

The invention discloses a micro-fluidic chip having a three-dimensional graphene interface, and a production method thereof. The micro-fluidic chip realizes the capture of single cells and two cells by designing micro-column capture tanks having different sizes through using hydrodynamics, and an impedance electrical signal during the micro-motion (such as cell adsorption, cell migration and cellproliferation) of single or two cells is quantitatively acquired in real time through a three-dimensional graphene micro-column electrode, so a new idea and a new technique are provided for cancer pathology and single cell detection. The production method of the micro-fluidic chip includes the following steps: producing a patterned gold electrode array, producing a three-dimensional graphene micro-column electrode array, producing a PDMS channel, and producing an openable PDMS cover plate. The micro-fluidic chip processed by the method has good biocompatibility, the production method has the advantages of mild conditions, simplicity, easiness in implementation, and controllable process parameters, and the detected single cell electric signal intensity of the micro-fluidic chip in the invention is twice that of an ordinary micro-fluidic chip.

Description

technical field [0001] The invention belongs to the field of micro-nano processing (MEMS) and sensor preparation, and in particular relates to a microfluidic chip with a three-dimensional graphene interface and a preparation method thereof. Background technique [0002] A microfluidic chip refers to a chemical or biological laboratory built on a chip of a few square centimeters. It integrates the basic operation units involved in the fields of chemistry and biology such as sample preparation, reaction, separation, detection, cell culture, sorting, lysis, etc. into a small chip to realize various tasks in conventional chemical or biological laboratories function. [0003] The main feature of microfluidic chips is that their effective structures (channels, reaction chambers and other functional components) containing fluids are micron-scale in at least one latitude. Due to the micron-scale structure, the fluid exhibits and produces special properties different from those in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M1/34G01N27/02
Inventor 刘爱萍陈本永王夏华吴化平邢赟许为中周奇
Owner TONGXIANG BEITE TECH CO LTD
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