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Self-cleaning electrodes for electrochemical biosensing

A sensing, electrochemical technology, applied in the field of electrochemical biosensing, can solve the problems of reduced detection efficiency, biological damage, poor ultraviolet light penetration effect, etc., and achieves great application prospects.

Active Publication Date: 2019-06-28
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing self-cleaning electrodes still have some deficiencies when they are applied in electrochemical biosensing: (1) Ultraviolet light is required to be irradiated during the cleaning process, which will cause irreparable damage to the irradiated organisms; (2) The penetration effect of ultraviolet light is poor. When cleaning the electrode, it is necessary to interrupt the detection and remove the electrode. Repeated disassembly and assembly of the electrode will not only delay the detection time, reduce the detection efficiency, but also greatly shorten the service life of the electrode

Method used

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  • Self-cleaning electrodes for electrochemical biosensing

Examples

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preparation example Construction

[0041] As a further preference, in the preparation method of the photosensitizer-upconversion nanoparticle composite particles, the specific steps of the step 1 are: weighing the YCl 3 ·6H 2 O, YbCl 3 ·6H 2 O and ErCl 3 ·6H 2 O, under an argon atmosphere, heat to 160°C to remove water in the system, after cooling to room temperature, add oleic acid and oleylamine as solvents, heat the system to 150-200°C to obtain a transparent and uniform solution 1; weigh the NaOH and NH 4 F, dissolved in methanol to obtain solution 2; quickly add the solution 2 to the solution 1, stir evenly, heat the system to 100°C to remove the methanol in the system, then quickly heat the system to 300°C, and react for 60 -90 min, cooling to room temperature; adding acetone to the reacted solution to precipitate product 1, washing the product 1 with a mixture of ethanol and methanol to obtain the up-converting nanoparticles. It should be noted that the up-conversion nanoparticles prepared by adopt...

Embodiment 1

[0047] With the ITO electrode as the conductive substrate, the ITO electrode was placed in a 0.1mg / mL reduced graphene oxide solution, a voltage of -1.0V was applied to the ITO electrode, and the deposition was continued for 1000s, so that a layer of reduced graphene oxide was deposited on the surface of the ITO electrode, and then Place the deposited ITO electrode in an aqueous solution of 8mg / mL photosensitizer-upconversion nanoparticle composite particles, apply a voltage of -1.0V, and continue to deposit for 1000s, so that a layer of photosensitizer-upconversion nanoparticle composite is deposited on the surface. particles to obtain self-cleaning electrodes for electrochemical biosensing.

[0048] Wherein, the preparation method of the photosensitizer-up-conversion nanoparticle composite particle is:

[0049] Step 1: Prepare NaYF 4 upconverting nanoparticles

[0050] (1) Weigh 0.5mmol YCl 3 ·6H 2 O, 0.1mmol YbCl 3 ·6H 2 O and 0.01 mmol ErCl 3 ·6H 2 O, under an argo...

Embodiment 2

[0062] With the ITO electrode as the conductive substrate, the ITO electrode was placed in a 0.55 mg / mL reduced graphene oxide solution, a voltage of -1.0V was applied to the ITO electrode, and the deposition was continued for 1500 s, so that a layer of reduced graphene oxide was deposited on the surface of the ITO electrode, and then Place the deposited ITO electrode in an aqueous solution of 8mg / mL photosensitizer-upconversion nanoparticle composite particles, apply a voltage of -1.0V, and continue to deposit for 1500s, so that a layer of photosensitizer-upconversion nanoparticle composite is deposited on the surface. particles to obtain self-cleaning electrodes for electrochemical biosensing.

[0063] Wherein, the preparation method of the photosensitizer-up-conversion nanoparticle composite particle is:

[0064] Step 1: Prepare NaYF 4 upconverting nanoparticles

[0065] (1) Weigh 0.75mmol YCl 3 ·6H 2 O, 1.05mmol YbCl 3 ·6H 2 O and 0.03 mmol ErCl 3 ·6H 2 O, under an...

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Abstract

The invention discloses a self-cleaning electrode for electrochemical biosensing. The electrode comprises a conductive substrate, wherein reduced graphite oxide and photosensitizer-upconversion nanoparticle composite particles are sequentially deposited on the surface of the conductive substrate; the photosensitizer-upconversion nanoparticle composite particles comprise upconversion nanoparticles,the outer surfaces of the upconversion nanoparticles are sequentially coated with dense silicon dioxide layers and mesoporous silicon dioxide layers, and photosensitizer molecules are adsorbed in mesopores of the mesoporous silicon dioxide layers. When the self-cleaning electrode for electrochemical biosensing is irradiated by near infrared rays with high transmittance, visible light can be emitted by the upconversion nanoparticles in the photosensitizer-upconversion nanoparticle composite particles deposited on the surface to activate the photosensitizer molecules to generate oxygen free radicals with high reactivity to implement in-situ self-cleaning of the electrode. Moreover, during cleaning, the electrode is irradiated by virtue of the near infrared rays with high transmittance, so that the electrode is not required to be repeatedly disassembled and assembled, and damage to an organism is prevented.

Description

technical field [0001] The invention belongs to the technical field of electrochemical biosensing, in particular to a self-cleaning electrode for electrochemical biosensing. Background technique [0002] Electrochemical sensors are widely used in biosensing due to their fast response, high sensitivity, and high temporal and spatial resolution. However, when electrochemical sensors are used for real-time detection in living organisms, their electrodes are easily contaminated and passivated by intermediates or polymerization products generated by electrochemical reactions during detection. Especially, for the detection electrodes near the cultured cells, various secretions from the cells can seriously contaminate and passivate the electrodes, attenuate the signals of the electrodes, cause the electrodes to be unreliable, and lead to a significant drop in the utilization of the electrodes. [0003] At present, in order to avoid the pollution and passivation of electrodes, some...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/38
Inventor 姜磊张浩薛齐刘涵云燕照霞马洪超
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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