A kind of spider silk hydrolyzate and preparation method thereof

A technology for spider silk and hydrolyzate, which is applied in the field of spider silk hydrolyzate and its preparation, can solve the problems of harsh preparation conditions and low amino acid yield, and achieves the effects of simple operation, easy absorption and low energy consumption.

Active Publication Date: 2021-02-02
海南蛛王生物开发有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation conditions of this hydrolysis method are relatively harsh, and the amino acid yield is low.

Method used

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  • A kind of spider silk hydrolyzate and preparation method thereof
  • A kind of spider silk hydrolyzate and preparation method thereof
  • A kind of spider silk hydrolyzate and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] The preparation of embodiment 1 spider silk hydrolyzate

[0050] The hydrolysis process of the present embodiment spider silk hydrolyzate is:

[0051] (1) Pretreatment: Take 2g of spider silk, rinse it with purified water to remove impurities, and put it in an agate ball mill for crushing until the spider silk has a flocculent or powdery growth length of <1 mm.

[0052] (2) Hydrolysis: Put the pulverized spider silk into a 1L glass container, add 200mL of 20g / L sodium hydroxide solution, and hydrolyze for 5h at a temperature of 80°C.

[0053] (3) Centrifugation: Centrifuge the hydrolyzate at a rotational speed of 15000r / min for 20min.

[0054] (4) Collection: Collect the centrifuged hydrolyzate into a glass container.

[0055] (5) Hydrolysis: hydrolyze the unhydrolyzed spider silk again, the method is the same as (2).

[0056] (6) Neutralization: neutralize with 1mol / L hydrochloric acid solution to pH 4.5.

[0057] (7) Filtration: filter the neutralized hydrolyzate ...

Embodiment 2

[0059] The preparation of embodiment 2 spider silk hydrolyzate

[0060] The hydrolysis process of the present embodiment spider silk hydrolyzate is:

[0061] (1) Pretreatment: Take 2g of spider silk, rinse it with purified water to remove impurities, and put it in an agate ball mill for crushing until the spider silk has a flocculent or powdery growth length of <1 mm.

[0062] (2) Hydrolysis: Put the pulverized spider silk into a 1L glass container, add 200mL of 15g / L sodium hydroxide solution, and hydrolyze for 4.5h at a temperature of 85°C.

[0063] (3) Centrifugation: Centrifuge the hydrolyzate at a rotational speed of 15000r / min for 20min.

[0064] (4) Collection: Collect the centrifuged hydrolyzate into a glass container.

[0065] (5) Hydrolysis: hydrolyze the unhydrolyzed spider silk again, the method is the same as (2).

[0066] (6) Neutralization: neutralize with 1mol / L hydrochloric acid solution to pH 5.0.

[0067] (7) Filtration: filter the neutralized hydrolyzate ...

Embodiment 3

[0069] Example 3 Preparation of spider silk hydrolyzate

[0070] The hydrolysis process of the present embodiment spider silk hydrolyzate is:

[0071] (1) Pretreatment: Take 2g of spider silk, rinse it with purified water to remove impurities, and put it in an agate ball mill for crushing until the spider silk has a flocculent or powdery growth length of <1 mm.

[0072] (2) Hydrolysis: Put the pulverized spider silk into a 1L glass container, add 200mL of 25g / L sodium hydroxide solution, and hydrolyze for 4 hours at a temperature of 90°C.

[0073] (3) Centrifugation: Centrifuge the hydrolyzate at a rotational speed of 15000r / min for 20min.

[0074] (4) Collection: Collect the centrifuged hydrolyzate into a glass container.

[0075] (5) Hydrolysis: hydrolyze the unhydrolyzed spider silk again, the method is the same as (2).

[0076] (6) Neutralization: neutralize with 1mol / L hydrochloric acid solution to pH 5.5.

[0077] (7) Filtration: filter the neutralized hydrolyzate th...

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Abstract

The invention relates to the technical field of amino acid preparation, in particular to a spider silk hydrolyzate and a preparation method thereof. The spider silk hydrolyzate is prepared by the following steps: crush the spider silk, mix the crushed spider silk with sodium hydroxide solution, hydrolyze at 80-90°C for 4-5 hours, and centrifuge to obtain a hydrolyzate; adjust the hydrolyzate The pH value is 4.0-5.5, and the 200nm membrane is filtered to obtain the spider silk hydrolyzate. The amino acid hydrolysis yield of the preparation process of the present invention is 74.15%-80.89%, and the yield is >70%; in the hydrolyzate, methionine is not detected, except for phenylalanine and tyrosine, the yields of other amino acids are all >60% . That is, the distribution of amino acids in the hydrolyzate is basically consistent with that in spider silk. In the hydrolyzate, small molecule peptides with a molecular weight of 1000‑5000D accounted for 72.18% to 76.69%, indicating that the spider silk hydrolyzate not only maintains the original beauty and medical effects of moisturizing, repairing, firming and anti-wrinkle, but also is easily destroyed Human body absorbs.

Description

technical field [0001] The invention relates to the technical field of amino acid preparation, in particular to a spider silk hydrolyzate and a preparation method thereof. Background technique [0002] Dry skin refers to the phenomenon that the skin lacks moisture and makes people feel uncomfortable. The symptoms are mainly tight skin, dry and peeling parts of the skin, and itching all over the body after bathing. Age growth, climate change, lack of sleep, excessive fatigue, overheated bath water, and strong alkaline detergents are all important causes of dry skin. The main methods to keep the skin moisturized are: the surface of the skin is covered with oil to reduce the evaporation of skin water; to provide sufficient moisture to the skin; to improve the nutritional status of the skin and restore the skin's water retention function. The hydrating and moisturizing mask can provide sufficient moisture to the skin. When using it, apply it directly to the face to continuously...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K2/00C07K1/02
CPCC07K2/00
Inventor 朱一强李平曾青钦
Owner 海南蛛王生物开发有限公司
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