Unlock instant, AI-driven research and patent intelligence for your innovation.

The upstream fragment of pappa2 gene as a molecular marker for pig immune traits and its application

A molecular marker and trait technology, used in DNA/RNA fragments, recombinant DNA technology, and the determination/inspection of microorganisms. It can solve problems such as influence and achieve the effect of good specificity and high sensitivity.

Active Publication Date: 2020-12-29
广西扬翔股份有限公司 +1
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above research results all indicate that the PAPPA2 gene plays an important role in the body's immunity and growth process, and the mutation of the upstream sequence site of the gene may affect the maintenance of the animal's body's immunity and growth performance by regulating the activity of the gene's own expression

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • The upstream fragment of pappa2 gene as a molecular marker for pig immune traits and its application
  • The upstream fragment of pappa2 gene as a molecular marker for pig immune traits and its application
  • The upstream fragment of pappa2 gene as a molecular marker for pig immune traits and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Genotyping Detection

[0037] (1) Using phenol extraction method to extract ear tissue DNA of Duroc pig × Erhualian pig F2 population

[0038] 1) Grind the ear tissue of Duroc × Erhualian F2 population (provided by Guangdong Huanong Wens Animal Husbandry Co., Ltd.) in liquid nitrogen, add an equal volume of 1 × SET (1mL), proteinase K (10ng / mL) To a final concentration of 200ug / mL, sodium dodecyl sulfate (ie SDS, 10%) to a final concentration of 0.5%, shake well. Incubate overnight in a 55°C water bath for digestion.

[0039] 2) Add an equal volume of Tris-saturated phenol to the digested tissue sample, slowly invert the centrifuge tube for 15 minutes, centrifuge in a low-temperature refrigerated centrifuge at 4°C and 11,000 rpm for 10 minutes, carefully transfer the supernatant to another centrifuge tube, and label with corresponding mark.

[0040] 3) Add an equal volume of phenol / chloroform / isoamyl alcohol (volume ratio 25:24:1), slowly invert the centri...

Embodiment 2

[0074] Example 2: Application of the PAPPA2 molecular marker typing method in the correlation analysis of pig immune traits

[0075] (1) Correlation analysis between PAPPA2 molecular marker typing results and immune traits

[0076] The experimental pigs used for the detection and analysis of the association between genotype and immune traits come from the F2 generation population (conventional breed) of Duroc×Erhualian crossbred by Guangdong Huanong Wen's Animal Husbandry Co., Ltd. The DNA used for genotyping was extracted from the ear sample of the F2 generation of Duroc×Erhualian hybrid ("Duroc×Erhualian hybrid F2 generation" referred to as "pig" in the following text and table) was used for blood routine testing and The blood for flow cytometric analysis was collected from pigs aged 20, 33, 35 and 80 days. Pigs aged 20 and 33 were not inoculated with polymyocytes (PolyI:C), and piglets at the age of 35 were inoculated with polymyocytes (PolyI:C). :C) Blood samples were col...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of pig molecular marker screening and particularly relates to a PAPPA2 gene upstream segment serving as a pig immune trait related detection molecular marker and application. The molecular marker is obtained by cloning in an upstream sequence of a pig PAPPA2 gene. A nucleotide sequence of the pig immune trait related molecular marker obtained by screening is shown as SEQ ID NO:1, and an A / G allele mutation at a 52th basic group of the sequence results in SequenomMassARRAY SNP genotyping polymorphism. The invention discloses a molecular marker screening method and correlation analysis method application. A novel SNP molecular marker resource is provided for pig immune trait marker assisted selection.

Description

technical field [0001] The invention belongs to the technical field of molecular marker screening of pigs, and specifically relates to the upstream fragment of PAPPA2 gene as a molecular marker of pig immune traits and its application. Background technique [0002] Pig disease has become one of the key factors restricting the healthy development of the pig industry, especially the popularization of large-scale pig farms, and the prevention and treatment of epidemic diseases is the top priority in the process of pig breeding. However, often due to the weak ability to control certain diseases, the measures such as disease detection, diagnosis, prevention and extermination are not handled in time, resulting in frequent occurrence of pig diseases. The outbreak of the disease can directly lead to the death of live pigs, reduce the slaughter rate, and then affect the economic benefits of pig breeding. At the same time, the large-scale use of drugs for the prevention and treatment...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172
Inventor 李新云赵书红赵志超栾宇刘华珍刘向东朱猛进刘小磊马云龙余梅刘望宏施亮李家连钱平
Owner 广西扬翔股份有限公司