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Gold nanorod podophyllotoxin liposome, preparation method and use thereof

A technology of gold nanorods and podophyllotoxin, which is applied in liposome delivery, pharmaceutical formulations, and photodisintegration of drugs in the body, etc. It can solve problems such as poor photothermal stability, inability to achieve circulatory therapy, and strong cytotoxicity. Achieve good photothermal stability

Active Publication Date: 2018-02-09
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although gold nanorods have enhanced photothermal properties, their photothermal stability is poor, and cyclic therapy cannot be achieved, and cetyltrimethylammonium bromide (CTAB) is attached to their surface, which has strong cytotoxicity.

Method used

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  • Gold nanorod podophyllotoxin liposome, preparation method and use thereof
  • Gold nanorod podophyllotoxin liposome, preparation method and use thereof
  • Gold nanorod podophyllotoxin liposome, preparation method and use thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0038] The gold nanorod podophyllotoxin liposome in this embodiment is a nanocomposite obtained by binding the gold nanorod to the outer surface of the podophyllotoxin liposome containing a carboxyl group after being modified with a polyelectrolyte.

[0039] The preparation method of gold nanorod podophyllotoxin liposome in the present embodiment is as follows:

[0040] 1. Modification of gold nanorods

[0041] 20 mL of gold nanorod solution with a concentration of 200 μg / mL was centrifuged twice at 8000 rpm for 20 minutes each time, the supernatant was discarded, and 10 mL of deionized water was added to the precipitate to resuspend (solution concentration was 160 μg / mL), Under ultrasonic conditions, 10 mL of PSS solution with a concentration of 2 mg / mL (solvent is 6 mM NaCl solution) was added dropwise. After ultrasonic reaction for 10 min, it was allowed to stand still for 20 min. PSS was bound to the surface of gold nanorods by electrostatic adsorption, and the excess PSS...

Embodiment 2

[0047] Example 2: Detection of potential and particle size of gold nanorod podophyllotoxin liposomes

[0048] Take the gold nanorod podophyllotoxin liposome sample diluted 10 times to the original solution, and use a laser particle size analyzer (NanoZS-90, UK) to measure Zeta potential and particle size under a 633nm He-Ne laser. The sample was injected into a U-shaped tube with a gold-plated electrode, placed in a laser particle size analyzer, and its potential was measured in parallel three times at room temperature. Inject the sample into a disposable polystyrene tube, and measure the particle size of the sample in the same way. The Zeta potential of the gold nanorod podophyllotoxin liposomes measured in the experiment was +15.7mV, and the particle size was 129.8nm.

Embodiment 3

[0049] Example 3: Electron Microscopic Observation of Gold Nanorod Podophyllotoxin Liposomes

[0050] Take samples of GNRs@PSS@PLL and gold nanorod podophyllotoxin liposomes and drop them on the copper grid respectively, let it stand and dry, and use a transmission electron microscope (TEM ht7700, Hitachi, Japan) to observe and analyze the samples by TEM at an accelerating voltage of 100kV , see the test results figure 2 .

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Abstract

The invention discloses a gold nanometer podophyllotoxin liposome, a preparation method and use thereof. The gold nanometer podophyllotoxin liposome is a nano-composite obtained by causing a gold nanorod to be modified by a polyelectrolyte and then combined with the outer surface of the podophyllotoxin liposome containing carboxyl. The gold nanometer podophyllotoxin liposome is in a typical vesicle shape with a particle diameter of about 129.8 nm and a zeta potential of +15.7 mV, and has a high extinction coefficient (8.08Lg<-1>cm<-1>) and photothermal conversion efficiency (46.1%); and the gold nanometer podophyllotoxin liposome has no cytotoxicity and no hemolysis. The gold nanometer podophyllotoxin liposome has good photothermal stability under near-infrared irradiation and can achieverapid release; and the synergistic treatment of chemotherapy-photothermal therapy has a strong killing effect on tumor cells, and an effect thereof may not be limited to this.

Description

technical field [0001] The invention relates to a gold nanorod podophyllotoxin liposome and a preparation method and application thereof, belonging to the technical field of nano-biomedicine. Background technique [0002] Podophyllotoxin is a kind of arylnaphtholactone lignan extracted and isolated from Podophyllum genus of Berberaceae, which has strong biological activities, such as insecticidal, antifungal, antiviral, anti-inflammatory, and immunosuppressive , anti-rheumatism, anti-spasm, lowering blood fat, etc. In recent years, the anticancer activity of podophyllotoxin has also received more and more attention. The main mechanism of its anticancer effect is to bind to tubulin and then prevent the formation of mitotic spindle during the G2 / M phase of cell division. However, the practical application of podophyllotoxin for cancer therapy is unsatisfactory due to its poor water solubility, insufficient selectivity for cancer cells, and severe side effects. [0003] In th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/127A61K31/365A61K47/32A61K47/34A61K47/28A61K47/24A61K47/10A61P35/00
CPCA61K9/1273A61K31/365A61K41/0042A61K41/0052A61K47/10A61K47/24A61K47/28A61K47/32A61K47/34
Inventor 吴泽宇王婷婷宋永红陆杨陈天云苏杨李飞闫旭杨沂陈朋朋张文成
Owner HEFEI UNIV OF TECH
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