Method for detecting fluoroquinolones medicine based on magnetic nano material purification-carbon quantum dot fluorescence sensibilization

A technology of fluoroquinolones and carbon quantum dots, which is applied in the field of chemical analysis and detection, can solve the problems of serious interference and inability to complete the detection of fluoroquinolones, and achieve the effects of eliminating milk matrix interference, good purification effect, and low cost

Active Publication Date: 2018-02-13
KUNMING UNIV OF SCI & TECH
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Problems solved by technology

The use of carbon quantum dots for fluorescence sensitization of fluoroquinolones has been reported by our research group before, but due to the use of molecular fluorescence detection, the detection of various fluoroquinolones has serious interference and cannot be completed
At the same time, there is no report on the combination of carbon quantum dot fluorescence sensitization combined with high performance liquid chromatography detection

Method used

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  • Method for detecting fluoroquinolones medicine based on magnetic nano material purification-carbon quantum dot fluorescence sensibilization
  • Method for detecting fluoroquinolones medicine based on magnetic nano material purification-carbon quantum dot fluorescence sensibilization
  • Method for detecting fluoroquinolones medicine based on magnetic nano material purification-carbon quantum dot fluorescence sensibilization

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Experimental program
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Embodiment 1

[0025] Embodiment 1: Determination of the residual content of 3 kinds of fluoroquinolones of levofloxacin, lomefloxacin and gatifloxacin in milk, the specific operation steps are as follows:

[0026] (1) Preparation method of water-soluble sulfur-doped fluorescent carbon quantum dots (S-CQDs): Weigh 3.0 g poly(4-styrenesulfonic acid-co-maleic acid) sodium salt, add it to 100 mL ultrapure water , sonicated for 5 min to make it fully mixed to form a colorless transparent liquid, and transferred to a polytetrafluoroethylene reactor, heated at 220 °C for 5 h, cooled to room temperature naturally, and filtered with a filter membrane with a pore size of 0.22 μm. Dialysis was performed for 24 h with a dialysis bag with a molecular weight cut-off of 3500 Da to obtain water-soluble sulfur-doped fluorescent carbon quantum dots, which were stored in a refrigerator at 4 °C in the dark for future use;

[0027] (2) Preparation method of magnetic nanomaterials: Weigh 2.05 g of ferrous ammoni...

Embodiment 2

[0035] Embodiment 2: Determination of the residual content of three kinds of fluoroquinolones in milk, levofloxacin, lomefloxacin and gatifloxacin, the specific operation steps are as follows:

[0036] (1) Preparation method of water-soluble sulfur-doped fluorescent carbon quantum dots (S-CQDs): Weigh 4.0 g poly(4-styrenesulfonic acid-co-maleic acid) sodium salt, add it to 100 mL ultrapure water , ultrasonically for 8 minutes to make it fully mixed to form a colorless transparent liquid, and transferred to a polytetrafluoroethylene reaction kettle, heated at 250 ℃ for 6 hours, cooled to room temperature naturally, filtered with a filter membrane with a pore size of 0.22 μm, and then filtered with a cut-off A dialysis bag with a molecular weight of 3200Da was dialyzed for 24 hours to obtain water-soluble sulfur-doped fluorescent carbon quantum dots, which were stored in a refrigerator at 4 °C in the dark for future use;

[0037] (2) Preparation method of magnetic nanomaterials:...

Embodiment 3

[0041] Embodiment 3: Determination of the residual content of 3 kinds of quinolones in milk, levofloxacin, lomefloxacin and gatifloxacin, the specific operation steps are as follows:

[0042] (1) Preparation method of water-soluble sulfur-doped fluorescent carbon quantum dots (S-CQDs): Weigh 5.0 g poly(4-styrenesulfonic acid-co-maleic acid) sodium salt, add it to 100 mL ultrapure water , ultrasonically for 10 min to make it fully mixed to form a colorless transparent liquid, and transferred to a polytetrafluoroethylene reaction kettle, heated at 230 °C for 7 h, cooled to room temperature naturally, filtered with a filter membrane with a pore size of 0.22 μm, and then filtered with a cut-off A dialysis bag with a molecular weight of 3000Da was dialyzed for 24 hours to obtain water-soluble sulfur-doped fluorescent carbon quantum dots, which were stored in a refrigerator at 4 °C in the dark for future use;

[0043] (2) Preparation method of magnetic nanomaterials: Weigh 2.45 g of...

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Abstract

The invention discloses a method for detecting fluoroquinolones medicine based on magnetic nano material purification-carbon quantum dot fluorescence sensibilization. According to the method, a magnetic nano material is utilized as a purifying material, and a milk matrix is purified by magnetic solid phase microextraction; poly(4-styrene sulfonic acid-co-maleic acid)sodium salt is utilized as a carbon source and a sulfur source, a sulfur-doped fluorescent carbon quantum dot having a fluorescence sensibilization effect on the fluoroquinolones medicine is synthesized by a hydrothermal method, and the strength of a fluorescent signal enhanced by the sulfur-doped fluorescent carbon quantum dot has a certain linear relation with a concentration of the fluoroquinolones medicine; thus, a high-efficiency liquid chromatography for detecting the fluoroquinolones medicine is established. The prepared magnetic nano material has excellent dispersity and a better purification effect and eliminates interference of complex matrixes; furthermore, the sulfur-doped fluorescent carbon quantum dot has different degrees of fluorescence sensibilization effects on the fluoroquinolones medicine, and the high-efficiency liquid chromatography can be utilized to measure varieties of fluoroquinolones medicine at the same time. The method disclosed by the invention has the remarkable characteristics of highsensitivity, good repeatability and the like.

Description

technical field [0001] The invention relates to the technical field of chemical analysis and detection, in particular to a method for detecting fluoroquinolones in milk based on magnetic nanomaterial purification-carbon quantum dot fluorescence sensitization combined with high-performance liquid chromatography. Background technique [0002] Fluoroquinolones (QNS) are a class of artificially synthesized broad-spectrum bactericidal antibacterial drugs, because of their broad antibacterial spectrum, strong antibacterial effect, high bioavailability, low toxicity and side effects, and no cross-resistance with other antibacterial drugs, etc. characteristics and are widely used clinically. Due to the slow elimination of these drugs in animals, excessive use or improper use will cause drug residues in animal-derived products; human ingestion may easily lead to bacterial drug resistance and cause adverse reactions to the central nervous system and joints. Therefore, the residual pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N30/02
CPCG01N21/643G01N30/02
Inventor 杨亚玲王蒙华建豪
Owner KUNMING UNIV OF SCI & TECH
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