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Immunofluorescent quantitative test strip for detecting myohemoglobin

A technique of myoglobin and immunofluorescence, which is applied in the field of immunological detection, can solve the problems of long enzyme-linked immunoassay assay period, expensive instruments required for detection, and high cost of chemiluminescence, and achieve high sensitivity, good stability, and high-level Analyze the effect of recovery rate

Inactive Publication Date: 2018-02-16
ZHONGSHAN CHUANGYI BIOCHEM ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Radioimmunoassay has radioactive substances, which will cause radioactive pollution; enzyme-linked immunoassay has a long measurement cycle, which is not suitable for rapid detection in emergency departments, and has high professional requirements for operators; the cost of chemiluminescence is high, and the equipment required for detection is relatively expensive

Method used

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  • Immunofluorescent quantitative test strip for detecting myohemoglobin
  • Immunofluorescent quantitative test strip for detecting myohemoglobin
  • Immunofluorescent quantitative test strip for detecting myohemoglobin

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Embodiment 1 storage solution formula and preparation method thereof

[0025] A preferred formulation of the storage solution of the present invention: the mass concentration of PB is 20mM, the percentage concentration of BSA is 1.8%, the percentage concentration of Tween-80 is 0.5%, the percentage concentration of glucose is 0.5%, and the percentage concentration of glycine The percentage concentration of PEG4000 is 1%, the percentage concentration of PEG20000 is 1.5%, the percentage concentration of Proclin300 or sodium azide is 0.03%, and the pH is 7.5.

[0026] Preparation method of storage solution: Weigh 0.25g of glucose and dissolve it in 45mL of pure water. Add 1mL of 100mM PB stock solution prepared in advance, shake and mix well. Add 1g of glycine, 0.5g of PEG4000, 0.75g of PEG20000 and 0.9g of BSA successively, shake and mix well; add 0.25mL Tween-80 with a sample gun, mix well repeatedly; add 15μL of Proclin300, shake and mix well; adjust the pH to 7.5 with...

Embodiment 2

[0028] The preparation method of embodiment 2 myoglobin immunofluorescence quantitative test strip

[0029] The preparation process of the myoglobin immunofluorescence quantitative test strip is as follows:

[0030] (1) Preparation of storage solution: the mass concentration of PB is 20mM, the percentage concentration of BSA is 1.8%, the percentage concentration of Tween-80 is 0.5%, the percentage concentration of glucose is 0.5%, and the percentage concentration of glycine is 2%, the percentage concentration of PEG4000 is 1%, the percentage concentration of PEG20000 is 1.5%, the percentage concentration of Proclin300 is 0.03%, and the pH7.5 is prepared according to the above formula, mixed and then filtered and sterilized to obtain a storage solution;

[0031] (2) Preparation of the sample pad: soak the glass fiber membrane with the sample pad treatment solution for 10 minutes, place in a drying room at 37°C and 30% humidity, and dry for 3 hours to prepare the sample pad for ...

Embodiment 3

[0046] Prepare the control storage solution, the specific formula is: the mass concentration of PB is 50mM, the percentage concentration of BSA is 1%, the percentage concentration of Tween-80 is 1%, the percentage concentration of glucose is 1%, the percentage concentration of glycine 0.5%, the percentage concentration of PEG4000 is 2%, and the percentage concentration of Proclin300 is 0.3%.

[0047] The preferred storage solution prepared in Example 1 (hereinafter referred to as storage solution 2) is compared with the reference storage solution (hereinafter referred to as storage solution 1) for stabilizing performance and precision, and the implementation method and results are as follows:

[0048] Using storage solution 1 and storage solution 2, the fluorescent microspheres labeled with myoglobin (MYO) monoclonal antibody by the same process were sprayed and dried, assembled into 40 test strips, put in aluminum foil bags, and sealed with desiccant. One bag was stored in re...

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Abstract

The invention discloses an immunofluorescent quantitative test strip for detecting myohemoglobin. The prepared myohemoglobin immunofluorescent quantitative test strip has high standard curve correlation coefficient, high detection accuracy rate, good stability, high precision and high sensitivity. Storage liquid used by the immunofluorescent quantitative test strip has relatively high chromatography recovery rate, fluorescent microspheres hardly remain on a binding pad or run up to water absorption paper on the test strip, and almost all the fluorescent microspheres are captured. The immunofluorescent quantitative test strip disclosed by the invention is applicable to clinical detection and has the advantages of simple operation, rapid reaction, high sensitivity, strong specificity, applicability to field detection, economy and practicability.

Description

technical field [0001] The invention relates to the field of immunological detection, and more specifically relates to a quantitative test strip for detecting myoglobin immunofluorescence. Background technique [0002] Myoglobin (MYO) is a specific oxygen-binding protein of skeletal muscle and cardiac muscle, with a molecular weight of 17.8kD. Due to its small molecular weight, it is mainly distributed in cardiac muscle and skeletal muscle tissue, accounting for about 0.1% to 0.2% of the total muscle. When myocardial or skeletal muscle is injured, MB is released from the muscle tissue into the circulating blood, and can be filtered by the glomeruli to appear in the urine. Therefore, the determination of MB in blood and urine can be used for the diagnosis of some myopathy and heart disease, such as acute myocardial infarction (AMI), acute muscle injury, acute and chronic renal failure, severe congestive heart failure, long-term shock, muscular dystrophy, diseases such as mus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/72
CPCG01N33/72
Inventor 何平梁婷婷李冰陈润文周琼华
Owner ZHONGSHAN CHUANGYI BIOCHEM ENG