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Preparation method and application of soluble dietary fiber

A dietary fiber and soluble technology, applied in the fields of application, food science, tea, etc., can solve environmental pollution and other problems, achieve high-efficiency extraction, sweet and sour taste, and lower cholesterol

Inactive Publication Date: 2018-02-23
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Traditional tea dietary fiber extraction technology mostly uses chemical methods, but this method will cause pollution to the environment, and at the same time, chemical substances will be introduced into the extract

Method used

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  • Preparation method and application of soluble dietary fiber
  • Preparation method and application of soluble dietary fiber
  • Preparation method and application of soluble dietary fiber

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: 10 g of pulverized tea leaves were weighed and added to 200 mL of citric acid buffer solution (pH 6.4) containing 0.8 g of α-amylase, and enzymatically hydrolyzed in a water bath at 45° C. for 4 hours. Adjust the pH of the enzymolysis solution to 4.8, add 1.0 g of cellulase, and perform enzymolysis at 50° C. for 3 hours. Adjust the pH of the enzymolysis solution to 6.8, add 0.6 g of papain and perform enzymolysis at 60°C for 3 hours. After enzymolysis, the enzymolysis solution was boiled for 10 minutes, filtered, and the supernatant was concentrated, precipitated with 3 times the volume of 95% ethanol for 9 hours, and centrifuged at 4000 r / min for 10 minutes. Evaporate the precipitated part after centrifugation to near dryness, and then dry to obtain soluble dietary fiber solid. Take 0.4g compound stabilizer (sodium carboxymethylcellulose and xanthan gum mass ratio 3:1) and mix evenly with 12g white granulated sugar, add 6g of soluble dietary fiber solid pre...

Embodiment 2

[0039] Example 2: 100 g of pulverized tea leaves and 7 g of α-amylase were dissolved in 2000 mL of citric acid buffer (pH 6.4), and hydrolyzed in a water bath at 48° C. for 5 hours. Adjust the pH of the enzymolysis solution to 4.8, add 10 g of cellulase, and perform enzymolysis at 50° C. for 3 hours. Adjust the pH of the enzymolysis solution to 7.0, add 7 g of papain and perform enzymolysis at 56° C. for 2.5 hours. After enzymolysis, the enzymolysis solution was boiled for 10 minutes, filtered, the supernatant was concentrated, ethanol precipitation with 2 times the volume of 95% ethanol for 8 hours, and centrifugation at 4000r / min for 10 minutes. Evaporate the precipitated part after centrifugation to near dryness, and then dry to obtain soluble dietary fiber solid. Take 5g of compound stabilizer (mass ratio of sodium carboxymethylcellulose to xanthan gum: 2:1) and mix evenly with 100g of white granulated sugar, add 64g of soluble dietary fiber solids, add 3g of citric acid,...

Embodiment 3

[0040] Example 3: 100 g of pulverized tea leaves and 5 g of α-amylase were dissolved in 2000 mL of citric acid buffer (pH 6.6), and hydrolyzed in a water bath at 48° C. for 5 hours. Adjust the pH of the enzymolysis solution to 4.4, add 6 g of cellulase, and perform enzymolysis at 50° C. for 3 hours. Adjust the pH of the enzymolysis solution to 7.0, add 9 g of papain and perform enzymolysis at 56°C for 2.5 hours. After enzymolysis, the enzymolysis solution was boiled for 10 minutes, filtered, and the supernatant was concentrated, precipitated with 4 times the volume of 95% ethanol for 8 hours, and centrifuged at 4000 r / min for 10 minutes. Evaporate the precipitated part after centrifugation to near dryness, and then dry to obtain soluble dietary fiber solid. Take 2g of compound stabilizer (mass ratio of sodium carboxymethylcellulose to xanthan gum: 2:1) and mix evenly with 50g of white granulated sugar, add 30g of soluble dietary fiber solids, add 1g of citric acid, 0.4g of vi...

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Abstract

The invention discloses a preparation method for soluble dietary fiber. The preparation method comprises the following steps: (1) subjecting tea leaf leftovers to drying, grinding and sieving; (2) adding alpha-amylase into the sieved tea leftovers for enzymolysis, adjusting the pH value of a solution to 6.0 to 6.8, and then carrying out enzymolysis at 40 to 50 DEG C for 4 to 6 h; (3) adjusting thepH value of the solution to 4.2 to 5.0, adding cellulase, and then carrying out enzymolysis at 45 to 55 DEG C for 1 to 3 h; (4) adjusting the pH value of the solution obtained in the step (3) to 6.6to 7.0, adding papain, and then carrying out enzymolysis at 55 to 60 DEG C for 1 to 3 h; and (5) boiling the solution for 5 to 10 min, then carrying out filtering, subjecting a clear solution to concentration, then carrying out alcohol precipitation with 95% ethanol accounting for 2 to 4 times the volume of the concentrate for 8 to 10 h, carrying out centrifugation at a speed of 4000 r / min for 10minutes, subjecting a precipitated part obtained after centrifugation to evaporation until the precipitated part is nearly dry, and then drying the precipitated part so as to obtain solid soluble dietary fiber. A beverage prepared from the prepared soluble dietary fiber retains functional ingredients in tea leaves, has high stability and is suitable for large-scale production.

Description

technical field [0001] The invention relates to a food auxiliary material and its preparation method and application, in particular to a soluble dietary fiber and its application. Background technique [0002] Dietary fiber refers to the sum of edible plant cells, lignin, polysaccharides and related substances that cannot be digested and absorbed by the human digestive system. From the perspective of specific chemical components, dietary fiber includes cellulose, hemicellulose, pectin, glucan sugar, lignin, etc. Dietary fiber is essential to a healthy diet. It maintains a healthy digestive system. Adequate intake of dietary fiber can also prevent diabetes, cardiovascular disease, cancer and other diseases. Dietary fiber can be divided into two categories, soluble dietary fiber (SDF) and insoluble dietary fiber (IDF). SDF is soluble in water, derived from pectin, alginate, konjac, etc., and has the effect of lowering blood fat and blood sugar. [0003] Tea is a low-calorie,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23F3/22A23F3/16
CPCA23F3/16A23F3/166A23F3/225
Inventor 李树炎唐迪洪文龙
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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