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Extraction and purification method for high-purity purple sweet potato anthocyanin

A purple potato anthocyanin and purification method technology, applied in the field of high-purity purple potato anthocyanin extraction and purification, can solve the problem of low upgrading, and achieve the effects of reducing solvent consumption, shortening extraction time, and improving product purity

Inactive Publication Date: 2018-02-23
安徽泰德康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention patent uses ultrafiltration membrane to remove impurities from the purple sweet potato concentrate after the initial purification of the resin. Ultrafiltration can only remove soluble sugar and protein with a large molecular weight in the concentrate, and most of the molecular weight is close to that of anthocyanins. The small molecule sugar and protein cannot be removed, and although the purity of the final product is improved, the improvement is not high

Method used

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  • Extraction and purification method for high-purity purple sweet potato anthocyanin
  • Extraction and purification method for high-purity purple sweet potato anthocyanin

Examples

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Effect test

Embodiment 1

[0053] Such as figure 1Shown, present embodiment adopts 500kg purple potato to extract anthocyanin example, adopts the following steps:

[0054] (1) Cleaning and peeling

[0055] Take 500kg of fresh purple sweet potatoes, wash and remove stones with a paddle cleaner, and then transfer them to a brush cleaning and peeling machine for deep cleaning and peeling.

[0056] (2) Filing and grinding

[0057] After the peeled purple sweet potato is preliminarily crushed by a crusher, it is then crushed by a rasping machine, so that the cells of the purple sweet potato are fully broken.

[0058] (3) Extraction Add acid aqueous solution to the crushed purple sweet potato cells for extraction, adjust the pH of the extraction solution to 2.0, the ratio of solid to liquid is 1:3, and extract for 1 hour to obtain the extraction solution.

[0059] (4) centrifugal

[0060] The extract is centrifuged with a horizontal spiral centrifuge, and solid-liquid separation is performed to obtain an ...

Embodiment 2

[0080] Such as figure 2 As shown, the present embodiment adopts the commercially available 100g purple sweet potato anthocyanin crude product deep purification example, comprising the following steps:

[0081] (1) Preparation of anthocyanin solution

[0082] Dissolve 100 g of commercially available purple potato anthocyanins in pure water to prepare an anthocyanin solution with a solid content concentration of 20%.

[0083] (2) Yeast activation

[0084] Dried Saccharomyces cerevisiae was added to 5% sugar water at a dosage of 5%, rehydrated at 38°C for 15min, and then rapidly cooled to 30°C for 1h activation.

[0085] (3) fermentation

[0086] Adjust the pH value of the anthocyanin solution to 4.5, control the temperature at 60°C, add glucoamylase for enzymolysis, the amount of glucoamylase added is 15u / ml solution, after 2 hours of enzymolysis, adjust the temperature of the anthocyanin solution to 30°C, add The activated Saccharomyces cerevisiae was subjected to aerobic ...

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Abstract

The invention aims to provide an extraction and purification method for high-purity purple sweet potato anthocyanin, in order to solve the problems of low extraction efficiency, easy polluted film filtration, low product purity, and the like, in the prior art. The method comprises the following steps: cleaning and peeling the raw materials; milling and crushing; extracting; centrifuging; performing first-level enzymolysis; absorbing resins; eluting; concentrating; performing second-level enzymolysis; performing yeast fermentation; filtering; performing spray drying, thus obtaining a high-concentration anthocyanin product. The purple sweet potato is milled and crushed after the cleaning and peeling process, so that the technology is capable of increasing the extraction rate of anthocyanin,reducing extraction time and reducing solvent consumption, namely, reducing the final handling capacity of the wastewater; the extracting solution is subjected to enzymolysis with pectinase, so that the flux of the membrane filtration is promoted and the membrane flux in the running process of the membrane is basically kept stable; yeast fermentation is adopted for removing the sugar from the anthocyanin resin purified solution, so that the product purity is greatly promoted.

Description

technical field [0001] The invention belongs to a method for extracting and purifying anthocyanins, in particular to a method for extracting and purifying high-purity purple potato anthocyanins. Background technique [0002] With people's in-depth understanding of the hazards of synthetic pigments and the demand for health, natural pigments are becoming more and more popular because they are natural, safe and non-toxic, and also take into account health care functions. They are widely used in the food and health care products industry, and the market The outlook is bright. [0003] Purple potato anthocyanin is a pure natural water-soluble pigment, the main component is anthocyanin compounds, in addition to being a good colorant, it also has the functions of scavenging free radicals, anti-oxidation, anti-mutation, etc., and is widely used in food and health products industries . [0004] Chinese Invention Patent Publication No. CN100535058C discloses a production process fo...

Claims

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Application Information

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IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 胡东余伟伟刘伦发黄宁
Owner 安徽泰德康科技有限公司
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