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Production technology of green tea extract with high EGCG content

A technology of green tea extract and production process, which is applied in the field of production process of green tea extract with high EGCG content, can solve the problems of unsuitability for large-scale industrial production, complicated process, and large consumption of reagents, so as to save production time and simplify production process. The effect of extracting operation steps and easy operation

Inactive Publication Date: 2018-02-23
浙江天草生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the chemical structure and properties of each component in catechin are very similar, it is difficult to separate them by general methods.
At the same time, although column chromatography and semi-preparative high-performance liquid chromatography can effectively separate different components in catechins, these processes are complicated, the consumption of reagents is large, the price is expensive, the preparation amount is small, and the cycle is long, so they are not suitable for use. large-scale industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] (1) Extraction: Put 100g of green tea leaves, tea powder, and pruned branches into an extractor, add 10 times the volume of purified water, add 2‰ of citric acid, heat to 80°C, and extract for 45 minutes. The green tea extract in the filter is filtered, and filter residue and filtrate are collected respectively;

[0069] The filter residue was leached twice according to the above conditions, then the filtrate was collected by filtration, and the two filtrates were combined to obtain 1.8 L of green tea extract.

[0070] (2) Adsorption: wash the green tea extract with a macroporous resin AB-8 chromatography column with a diameter-to-height ratio of 1:10, and then wash it with 10% ethanol of 2 times the column volume to remove non-adsorbed substances, and then wash it with 3 times the column volume Wash with 70% ethanol by volume, desorb the active ingredient, and collect 300 mL of the eluent.

[0071] (3) Concentration: Concentrate the collected eluent by rotary evaporat...

Embodiment 2

[0079] (1) Extraction: Put 250kg of green tea leaves, tea powder, and pruned branches into an extraction tank, add 10 times the volume of purified water, add 2‰ of citric acid, heat to 80°C, extract for 45 minutes, and extract The green tea extract is filtered to collect filter residue and filtrate respectively;

[0080] The filter residue was leached twice under the same conditions, filtered through plate and frame, and the two filtrates were combined to obtain 5000L green tea extract.

[0081] (2) Adsorption: Put the green tea extract on a macroporous resin LX-8 chromatography column with a diameter-to-height ratio of 1:10. After loading, wash with 10% ethanol for 2 times the column volume to remove non-adsorbed substances. Rinse with 3 times column volume of 70% ethanol again, desorb the active ingredient, and collect 7500 L of eluate.

[0082] (3) Concentration: Add the collected eluate to the concentrator, concentrate at 65°C, and concentrate to a concentration of 1.08kg...

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Abstract

The invention provides a production technology of a green tea extract with a high EGCG content. In the production technology of the invention, the green tea extract is prepared by methods of digestion, adsorption, concentration, extraction, concentration drying, crystallization, drying and pulverization. The production technology of the green tea extract with the high EGCG content is simple and convenient, has high separation efficiency and short production cycle, and is low in cost and is suitable for large-scale and industrial production of the green tea extract with the high EGCG content.

Description

technical field [0001] The invention relates to the field of natural product extraction, in particular to a production process of green tea extract with high EGCG content. Background technique [0002] Green tea is made without fermentation, so it retains more natural substances of fresh leaves, and contains tea polyphenols, caffeine, amino acids, vitamins and other nutrients. Among them, tea polyphenol is a kind of natural antioxidant, its main component is catechin, and catechin is composed of more than six kinds of monomers, mainly including catechin (C), epicatechin ( EC), epigallocatechin (EGC), gallocatechin (GC), epicatechin gallate (ECG), epigallocatechin gallate (EGCG) and the like. Studies have shown that EGCG is the main highly active ingredient in green tea, which has various health care and pharmacological effects such as scavenging free radicals, anti-oxidation, anti-aging, lowering blood fat and cholesterol, anti-atherosclerosis and antibacterial. Therefore,...

Claims

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Application Information

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IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 管成林黄利李鹏飞程勇
Owner 浙江天草生物科技股份有限公司
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