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Vibrio splendidus colloidal gold immunochromatograohic assay test strip and preparation method thereof

An immunochromatographic test paper, Vibrio brilliant technology, applied in the direction of analytical materials, biological material analysis, measuring devices, etc., can solve the problems of no application, etc., and achieve the effect of simple operation, strong specificity, and rapid detection

Active Publication Date: 2018-02-23
DALIAN OCEAN UNIV
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, since there is no monoclonal antibody against Vibrio splendidus that is suitable for colloidal gold immunochromatographic test strips so far, microbiological detection techniques, immunofluorescence techniques, enzyme-linked immunosorbent assays, bacterial colloidal gold immunochromatographic test strips have not been applied in the detection of Vibrio splendidus

Method used

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  • Vibrio splendidus colloidal gold immunochromatograohic assay test strip and preparation method thereof

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Embodiment Construction

[0024] The Vibrio brilliant colloidal gold immunochromatographic test strip of the present invention has the same structure as the prior art, and has a base plate 5 on which a sample pad 4, a gold standard pad 3, The nitrocellulose membrane 2 and the water-absorbing pad 1 overlap each other. The difference from the prior art is that the colloidal gold-labeled Vibrio splendidus flagellin FlgT monoclonal antibody is sprayed on the gold standard pad 3, and the detection line a coated with the Vibrio splendidus polyclonal antibody and the The quality control line b composed of goat anti-mouse IgG, said Vibrio splendidus flagellin FlgT monoclonal antibody is prepared by immunizing mice with hybridoma cell 4A5 with the deposit number of CGMCC No. 14311, and purifying ascites of mice.

[0025] Vibrio brilliant colloidal gold immunochromatographic test strip preparation method of the present invention, carry out according to the following steps:

[0026] 1. Prepare the monoclonal ant...

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Abstract

The invention discloses a vibrio splendidus colloidal gold immunochromatograohic assay test strip capable of rapidly detecting pathogenic bacteria of stichopus japonicus 'bark rot' and a preparation method thereof. The test strip comprises a bottom plate, wherein a sample pad, a gold-labeled pad, a nitrocellulose membrane and a water absorbent pad are sequentially adhered onto the bottom plate from one end from top to bottom in a stepped manner; a colloidal gold labeled vibrio splendidus flagellin FlgT monoclonal antibody is sprayed onto the gold-labeled pad; a detection line a coated with a vibrio splendidus polyclonal antibody and a quality control line b composed of goat anti-rat IgG are arranged on the nitrocellulose membrane; and the vibrio splendidus flagellin FlgT monoclonal antibody is prepared by immunizing mice by hybridoma cells 4A5 with a collection number of CGMCC No. 14311, and purifying mouse ascites obtained.

Description

technical field [0001] The invention belongs to the technical field of rapid immunological detection of pathogenic bacteria in aquatic animals, in particular to a colloidal gold immunochromatographic test strip and a preparation method for rapid detection of Vibrio splendidus, the pathogenic bacteria of sea cucumber "transfer skin disease". Background technique [0002] Vibrio splendidus belongs to the Vibrio family ( Vibrionaceae ), Vibrio ( Vibrio ), is sea cucumber ( Apostichopus japonicus ) The main pathogenic bacteria of "skin disease" (rot skin syndrome). The disease has the characteristics of strong infectivity and wide spread, and can cause symptoms such as large-scale body surface ulcers, mouth swelling and ulceration, body atrophy, and viscera discharge in sea cucumbers. In addition, Vibrio splendidus can also cause disease and even death of various fish, bivalve molluscs, crustaceans and echinoderms, causing bleeding spots on both sides of the infected fish, ...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/558G01N33/532
CPCG01N33/532G01N33/558G01N33/56911G01N2333/28
Inventor 李强王博雅黎睿君叶仕根
Owner DALIAN OCEAN UNIV
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