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A kit for measuring activated partial thromboplastin time (aptt)

A technology of thromboplastin time and thromboplastin is applied in the field of kits for determining activated partial thromboplastin time (APTT) in blood, which can solve the problems of large differences in measurement results and unstable quality of kits, and avoid bottle Differences between, avoid large differences, and ensure the effect of stability

Active Publication Date: 2019-02-26
山东艾科达生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems of unstable quality of existing kits for detecting activated partial thromboplastin time (APTT) and large differences in measurement results, the present invention provides a reagent for measuring activated partial thromboplastin time (APTT) that is liquid and can exist stably Kits, different batches of kits have little difference in test results

Method used

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  • A kit for measuring activated partial thromboplastin time (aptt)
  • A kit for measuring activated partial thromboplastin time (aptt)
  • A kit for measuring activated partial thromboplastin time (aptt)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Preparation of kit for measuring activated partial thromboplastin time (APTT)

[0038] formula:

[0039] R1 reagent: 0.1mM ellagic acid, 0.25% rabbit cephalin, 3% dextran 20, 0.5% Tween20, 1% bovine serum albumin, 20mM pH 7.6 Tris-HCl buffer, 0.02% ProClin 300.

[0040] R2 reagent: 25mM calcium chloride, 0.01% thimerosal.

[0041] Preparation:

[0042] (1) Weigh rabbit cephalin, dextran 20, Tween20, bovine serum albumin, ProClin 300 and dissolve in 20mMpH 7.6 Tris-HCl buffer solution, stir well and add ellagic acid to the required concentration to prepare R1 reagent;

[0043] (2) Weigh calcium chloride and thimerosal respectively and add them into sterile water to prepare R2 reagent.

Embodiment 2

[0044] Example 2 Detection of activated partial thromboplastin time

[0045] Adopt the kit of embodiment 1 and commercially available reagent to measure the same plasma sample with full-automatic hemagglutination instrument, the main parameter settings of the instrument are shown in Table 1; record the activated partial thromboplastin time, the repeatability of reagent of the present invention and commercially available reagent The comparison results are shown in Table 2. As can be seen from the data in the table, compared with commercially available reagents, the reagent of the present invention has a smaller coefficient of variation and better repeatability.

[0046] Open the bottle of the reagent of the present invention and the commercially available reagent at the same time, the commercially available reagent is used after reconstitution of freeze-dried powder, the reagent of the present invention is used directly, and the quality control test is performed on the same coa...

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Abstract

A kit for measuring activated partial thromboplastin time (APTT) is provided. The kit includes R1 including an activator, partial thromboplastin, a stabilizer, an aseptic and a buffer liquid; and R2 that is a calcium chloride solution. The stabilizer includes dextran. In the R1, the concentration of the activator is 0.05-0.2 mM, the content of the partial thromboplastin is 0.1-0.5 wt%, the contentof the aseptic is 0.01-0.02 wt% and the content of the dextran is 2-4 wt%. All agents in the kit are liquid and stable so that reagent differences among bottles caused by freeze-drying and redissolving processes in preparation and application processes, thus avoiding large differences among measurement results. Stability of the reagents can be ensured without the need of freeze drying, and optimum validity of the reagents after bottle opening is at least a month, thus avoiding large differences among experiment results, reducing using amounts of the reagents, achieving instant use after bottle opening, and making operation rapider and simpler.

Description

technical field [0001] The invention belongs to the technical field of medical testing, in particular to a kit for measuring activated partial thromboplastin time (APTT) in blood. Background technique [0002] Determination of activated partial thromboplastin time (APTT) is an important indicator of coagulation function testing and an important indicator of preoperative examination. APTT determination is a sensitive and commonly used screening test for the endogenous coagulation system. It can also be used as a quantitative test for coagulation factors in the endogenous and common pathways. It can detect other plasma coagulation factors except factor VII, especially for factor VIII. , Ⅸ, Ⅺ, Ⅻ and the determination of prekallikrein. Meanwhile, APTT assay can be used for heparin therapy monitoring. Prolonged APTT: seen in decreased blood coagulation factors Ⅱ, Ⅴ, Ⅷ, Ⅸ, Ⅺ, Ⅻ, fibrinogen deficiency, enhanced fibrinolytic activity, presence of anticoagulant substances (such as ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/86G01N33/573
CPCG01N33/573G01N33/86G01N2333/974G01N2800/224
Inventor 谭柏清王醒赵志敏
Owner 山东艾科达生物科技有限公司
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