Activated partial thromboplastin time assay kit and preparation method thereof

A technology of thromboplastin time and reagent kit, which is applied in the biological field, can solve the problems of waste, reagent stability and measurement effect deterioration, and achieve the effects of saving dosage, avoiding differences between bottles, and avoiding large differences

Active Publication Date: 2017-08-29
武汉中太生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the deficiencies in the above-mentioned existing background technology, the present invention provides a kind of activated partial thromboplastin time assay kit and its preparation method, and this activated partial thromboplastin time assay kit eliminates the subpackaging and Freeze-drying and other steps avoid the problem of reagent stability and measurement effect deterioration caused by reconstitution during use, ensure the stability of the reagent, and avoid the waste of reagents, further improving accuracy, repeatability and stability Activated partial thromboplastin time assay reagent with better overall performance and easier operation

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  • Activated partial thromboplastin time assay kit and preparation method thereof

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Embodiment 1

[0025] Activated partial thromboplastin time determination kit, including phospholipid reagent and calcium chloride reagent, the two reagents are stored in different containers; the phospholipid reagent is composed of ellagic acid, phospholipid and buffer system, the content of ellagic acid is 2g / L , the content of phospholipids is 2.5wt%, and the content of each component in the buffer system is: Tris-Hcl of 1M, Kathon of 0.01wt%, polyethylene glycol of 1.0wt%, the rest are water, and the pH of the buffer system is 6.0; The working concentration of calcium chloride reagent is 15 mM. When using the kit of the present invention, the volume ratio of the plasma to be tested and the amount of phospholipid reagent is 1:0.85-1.1.

[0026] The method for preparing the above-mentioned activated partial thromboplastin time assay kit comprises the following steps: (1) preparing a buffer system, adjusting the pH to 6.0-8.0, adding a solid activator and mixing to obtain an initial mixed s...

Embodiment 2

[0029]Activated partial thromboplastin time assay kit, including phospholipid reagent and calcium chloride reagent, the two reagents are stored in different containers; the phospholipid reagent is composed of bentonite, rabbit cephalin and buffer system, and the content of bentonite is 0.05 g / L, the content of rabbit cephalin is 7wt%, and the content of each component in the buffer system is: the MPOS of 0.4M, the sodium azide of 0.1wt%, the polyethylene glycol of 0.8wt%, all the other are water; The pH of the system is 7.8, and the working concentration of calcium chloride reagent is 20mM.

[0030] The preparation method of the activated partial thromboplastin time assay kit is the same as in Example 1.

[0031] Apply the test kit obtained in this embodiment to measure the prothrombin time of the plasma to be tested on a semi-automatic hemagglutination instrument, the measurement method is the same as in Example 1, do ten parallel experiments, record the data, and analyze the...

Embodiment 3

[0033] Activated partial thromboplastin time determination kit, including phospholipid reagent and calcium chloride reagent, the two reagents are stored in different containers; phospholipid reagent is composed of solid activator, rabbit cephalin and buffer system, the content of solid activator is 1.0 g / L, the content of rabbit cephalin is 5wt%, and the content of each component in the buffer system is: the Hepes of 0.75M, the stabilizing agent of 0.05wt%, the polyethylene glycol of 0.3wt%, all the other are water; The pH of the solution is 6.0, and the working concentration of calcium chloride reagent is 35mM; wherein, the solid activator is a mixture of ellagic acid, kaolin and magnesium aluminum silicate with a quality of 1:0.5:2, and the stabilizer is Kathon, glycine, serum white 1:0.86:1.1 mixture of egg whites.

[0034] The preparation method of the activated partial thromboplastin time assay kit is the same as in Example 1.

[0035] Apply the test kit obtained in this...

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Abstract

The invention discloses an activated partial thromboplastin time measurement kit and a preparation method thereof. By the adoption of the activated partial thromboplastin time measurement kit, the steps of subpackaging, freeze-drying and the like in the preparation process are eliminated, and the condition that a greater difference between measurement results is caused due to between-bottle difference caused by freeze-drying and redissolving processes of reagents is avoided. The reagents used in the activated partial thromboplastin time measurement kit do not need to be freeze-dried, to guarantee the stability of the reagents and ensure that the optimal effectiveness of the decapped reagents lasts for at least one month, thus avoiding the greater difference between experimental results; meanwhile, the use amount of the reagents is reduced; the reagents can be used instantly after a bottle is opened, and the operation is quicker, simpler and more convenient.

Description

technical field [0001] The invention relates to an in vitro diagnostic kit and a preparation method thereof, in particular to an activated partial thromboplastin time measurement kit and a preparation method thereof, belonging to the field of biotechnology. Background technique [0002] The hemostatic function of the body is accomplished by the joint action of platelets, coagulation system, fibrinolytic system and vascular endothelial system. In the past, we used bleeding time as a screening test for hemostatic function defects, but because of the low standardization of operation, poor sensitivity, and inability to reflect the content and activity of coagulation factors, it has been replaced by coagulation function tests. Coagulation function tests mainly consist of four coagulation items - prothrombin time, activated partial thromboplastin time, thrombin time and fibrinogen content. The four blood coagulation items are important clinical inspection items in the laboratory ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/86
CPCG01N33/86
Inventor 马全新肖成陈莹
Owner 武汉中太生物技术有限公司
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