Application of total volatile oil of branches and leaves of Ledum palustre in controlling stored grain insects

A technology of total volatilization, branches and leaves, applied in application, pest control, insecticide, etc., can solve the problems of destroying the ecological balance of nature, affecting daily life, endangering human health, etc., achieves significant contact and fumigation activity, and the preparation method is simple Easy to implement and easy to control

Active Publication Date: 2018-03-06
INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the weakness and long-term unreasonable abuse of chemical pesticides, pests have developed resistance to chemical pesticides, and in the process of extensive use of

Method used

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  • Application of total volatile oil of branches and leaves of Ledum palustre in controlling stored grain insects
  • Application of total volatile oil of branches and leaves of Ledum palustre in controlling stored grain insects
  • Application of total volatile oil of branches and leaves of Ledum palustre in controlling stored grain insects

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Preparation method of total volatile oil from branches and leaves of Duxiang

[0035] In the present invention, there is provided a method for extracting the total volatile oil from the branches and leaves of Duxiang, but it is only used to achieve the purpose of the present invention. The extraction method is not limited to other extraction methods, such as supercritical CO 2 Extraction method (Lu Boqiong, Luo Shuqin, Zhang Liping. Supercritical CO 2 Extraction process optimization and component analysis of volatile oil from Duxiang. Modern Agricultural Science and Technology, 2014, 6: 173-178.).

[0036] 500-1000g duxiang branches and leaves are dried in the shade and crushed to 40-120 meshes. Weigh the crushed duxiang branches and leaves into a 3L round-bottom flask, add 1-2L of water, soak for 3-4h, and distill with steam distillation 3- After 4h, the obtained oil-water mixture was extracted with n-hexane. After the extract was dehydrated with anhydrous sodium ...

Embodiment 2

[0037] Example 2: GC-MS analysis and detection of the total volatile oil of the branches and leaves of Duxiang

[0038] The GC-MS analysis of the total volatile oil of the branches and leaves of Duxiang in Example 1 was detected by Agilent 6890N gas chromatograph and Agilent 5973N mass spectrometer.

[0039] HP-5MS capillary column (30m×0.25mm×0.25μm); FID detector; initial temperature 40°C (maintained for 2min), increasing to 280°C (maintained for 2min) at a heating rate of 10°C / min; inlet temperature 250 ℃; injection volume 1.0μL; splitless, helium carrier gas, flow rate 1mL / min.

[0040] Mass spectrometry analysis conditions: EI ionization source, interface temperature 280℃, ion source temperature 230℃, electron energy 70eV, scanning range is 30~500u.

[0041] Take 1μL of total volatile oil of Duxiang branch and leaf (100 times ethanol dilution) into the sample, analyze and identify with gas chromatography-mass spectrometry, search the NIST08 standard mass spectrum library through ...

Embodiment 3

[0046] Example 3: Determination of fumigation activity

[0047] During the test, the total volatile oil of the branches and leaves of Duxiang and acetone were mixed and diluted in a ratio of 1:15 (volume ratio). Take 1mL of diluted volatile oil and drop it on half a piece of filter paper (12.5cm), then roll the filter paper into a tube shape and fix it to the fumigation bottle with double-sided tape (made from a 500mL mineral water bottle after washing and drying, the inner side wall Coated with PTFE) bottle stopper, carefully plug the bottle stopper tightly so that the filter paper is hung in the fumigation bottle, taking care not to contact the bottle wall. Add 5g of wheat into the bottle and insert 30 test insects. Take the same amount of acetone dropwise on the filter paper strip as a control. Put it into a 28°C constant temperature incubator for cultivation. Observe and record the death of the test insects in each bottle every 24h, calculate the mortality and correct the ...

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Abstract

The invention relates to application of total volatile oil of branches and leaves of Ledum palustre in controlling stored grain insects, and belongs to the technical field of biological pesticides. Amedicament for preventing and controlling the stored grain pests provided by the present invention belongs to a plant-derived insecticide, is easy to decompose, does not pollute the environment, and is safe to humans and animals; the active ingredient of the medicament is the total volatile oil from the branches and leaves of the Ledum palustre, and has a variety of action mechanisms against the insect pests. The resistance to the medicament of the insects is not easy to produce. The medicament can be used for a long period of time. The medicament loses activity after being used for deinsectization, may not accumulate in the insects, and protects the ecological environment. The preparation method of the medicament is simple and easy to implement, the range of raw materials is wide, cost islow, the medicament is suitable for large-scale production, and the medicament can be widely used for the prevention and control of the stored grain pests.

Description

Technical field [0001] The invention relates to the application of the total volatile oil of the branches and leaves of Duxiang in the prevention and control of stored grain pests, and belongs to the technical field of biological pesticides. Background technique [0002] In the process of grain storage, the damage of stored grain pests is the most important factor affecting food security. my country's annual food loss caused by stored-grain pests accounts for about 4%-6% of the total stored grain, which is equivalent to about 30 billion yuan. In addition to causing serious economic losses, pests in stored grain will increase insect-eaten grains, reduce germination, and can cause heat and mildew of grain, thereby greatly reducing the commodity value and edible value of grain. [0003] At present, the methods to prevent and control stored grain pests mainly include physical and mechanical control methods and chemical control methods. Physical and mechanical control, that is, the ha...

Claims

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Application Information

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IPC IPC(8): A01N65/16A01N25/18A01P17/00A01P7/04
Inventor 张亮亮汪咏梅徐曼胡新宇王宏晓
Owner INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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