Application of Total Volatile Oil of Ledum Branches and Leaves in Controlling Stored Grain Pests

A technology of total volatilization, branches and leaves, applied in the direction of application, pest control, pesticides, etc., can solve the problems of destroying the ecological balance of nature, endangering human health, affecting daily life, etc., achieving significant contact and fumigation activity, easy to control, The effect of the simple and easy preparation method

Active Publication Date: 2020-07-03
INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the weakness and long-term unreasonable abuse of chemical pesticides, pests have developed resistance to chemical pesticides, and in the process of extensive use of chemical pesticides, the environment has been polluted, the ecological balance of nature has been destroyed, and human health has been endangered. seriously affect our daily life

Method used

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  • Application of Total Volatile Oil of Ledum Branches and Leaves in Controlling Stored Grain Pests
  • Application of Total Volatile Oil of Ledum Branches and Leaves in Controlling Stored Grain Pests
  • Application of Total Volatile Oil of Ledum Branches and Leaves in Controlling Stored Grain Pests

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: the preparation method of the total volatile oil of Ledum branch and leaf

[0035] Among the present invention, a kind of extracting method of total volatile oil of Ledum branch and leaf is provided, but only for realizing the total volatile oil of Ledum branch and leaf of the present invention, not limited to the extraction of this method also can adopt other extraction methods, as supercritical CO 2 Extraction method (Lu Boqiong, Luo Shuqin, Zhang Zhangping. Supercritical CO 2 Extraction process optimization and component analysis of Ledum volatile oil. Modern Agricultural Science and Technology, 2014, 6: 173-178.).

[0036]Dry 500-1000g of Ledum branches and leaves in the shade, crush them to 40-120 mesh, weigh the crushed Ledum branches and leaves in a 3L round bottom flask, add 1-2L of water, soak for 3-4h, and distill 3- After 4 hours, the obtained oil-water mixture was extracted with n-hexane, and the extracted product was dehydrated with anhydrous...

Embodiment 2

[0037] Embodiment 2: GC-MS analysis and detection of the total volatile oil of Ledum branch and leaf

[0038] The GC-MS analysis of the total volatile oil of the branches and leaves of Ledum in Example 1 was detected by Agilent 6890N gas chromatograph and Agilent 5973N mass spectrometer.

[0039] HP-5MS capillary column (30m×0.25mm×0.25μm); FID detector; initial temperature 40°C (keep 2min), rise to 280°C at a heating rate of 10°C / min (keep 2min); inlet temperature 250 ℃; injection volume 1.0 μL; splitless, helium carrier gas, flow rate 1mL / min.

[0040] Mass spectrometry conditions: EI ionization source, interface temperature 280°C, ion source temperature 230°C, electron energy 70eV, scan range 30-500u.

[0041] Take 1 μL (100-fold ethanol dilution) of the total volatile oil of the leaves of Ledum lanceolata as a sample, analyze and identify it by gas chromatography-mass spectrometry, search the NIST08 standard mass spectrum library through the HP-MSD chemical workstation, a...

Embodiment 3

[0046] Example 3: Determination of fumigation activity

[0047] During the test, the total volatile oil of Ledum branch and leaf and acetone were mixed and diluted in a ratio of 1:15 (volume ratio) before use. Take 1mL of the diluted volatile oil and drop it on half a piece of filter paper (12.5cm), then roll the filter paper into a cylinder and fix it in a fumigation bottle (made by washing and drying a 500mL mineral water bottle, the inner wall Coated with polytetrafluoroethylene) on the bottle stopper, carefully stopper the bottle stopper so that the filter paper is suspended in the fumigation bottle, taking care that it does not come into contact with the bottle wall. Add 5g of wheat into the bottle, and insert 30 test insects. Add the same amount of acetone dropwise on the filter paper as a control. Place them in a constant temperature incubator at 28°C. Observe and record the death of test insects in each bottle every 24 hours, and calculate the death rate and correct...

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Abstract

The invention relates to application of total volatile oil of branches and leaves of Ledum palustre in controlling stored grain insects, and belongs to the technical field of biological pesticides. Amedicament for preventing and controlling the stored grain pests provided by the present invention belongs to a plant-derived insecticide, is easy to decompose, does not pollute the environment, and is safe to humans and animals; the active ingredient of the medicament is the total volatile oil from the branches and leaves of the Ledum palustre, and has a variety of action mechanisms against the insect pests. The resistance to the medicament of the insects is not easy to produce. The medicament can be used for a long period of time. The medicament loses activity after being used for deinsectization, may not accumulate in the insects, and protects the ecological environment. The preparation method of the medicament is simple and easy to implement, the range of raw materials is wide, cost islow, the medicament is suitable for large-scale production, and the medicament can be widely used for the prevention and control of the stored grain pests.

Description

technical field [0001] The invention relates to the application of total volatile oil of Ledum branch and leaf in preventing and controlling stored grain pests, and belongs to the technical field of biological pesticides. Background technique [0002] In the process of grain storage, the harm of stored grain pests is the most important factor affecting food security. In my country, the annual grain loss caused by stored grain pests accounts for about 4%-6% of the total stored grain, which is equivalent to about 30 billion yuan. In addition to causing serious economic losses, the damage of stored grain pests will increase the number of worm-eaten grains, reduce the germination rate, and can cause grain heating and mildew, thereby greatly reducing the commodity value and edible value of grain. [0003] At present, the methods of controlling stored grain pests mainly include physical and mechanical control methods and chemical control methods. Physical and mechanical control,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/16A01N25/18A01P17/00A01P7/04
Inventor 张亮亮汪咏梅徐曼胡新宇王宏晓
Owner INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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