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EGF/vitamin E acetate-cyclodextrin liposome as well as preparation method, use method and application thereof

A kind of cyclodextrin and acetate technology, which is applied to the preparation of EGF/vitamin E acetate-cyclodextrin liposome, the use method and application of EGF/vitamin E acetate-cyclodextrin liposome It can solve the problems of low bioavailability, easy inactivation, restricted promotion and use, etc., and achieve the effects of convenient use, easy packaging, and improved stability and bioavailability

Inactive Publication Date: 2018-03-13
YANTAI KANGDAER PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, EGF has poor stability in vitro, short half-life in vivo, easy inactivation, and low bioavailability; vitamin E acetate is a fat-soluble active substance with extremely poor water solubility and is easily oxidized when exposed to light. Therefore, both are difficult to be directly applied It is used in cosmetics to exert anti-aging ability, which seriously restricts the large-scale promotion and use in various fields

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Weigh 4.00g of β-cyclodextrin, add 100ml of distilled water to fully dissolve it; take another 0.50g of vitamin E acetate, stir at 35°C for 6h, and centrifuge the dispersion of inclusion compound at 70000g for 60min , the supernatant is an aqueous solution of vitamin E acetate-cyclodextrin inclusion compound, the supernatant is concentrated in vacuum, and the concentrate is dried in a vacuum oven at 40°C and 0.08MPa for 6 hours to obtain powdered vitamin E acetate -Cyclodextrin inclusion compound, the inclusion rate reaches 86.57% through measurement.

[0036](2) 10.25g of soybean lecithin and 2.50g of cholesterol were mixed and dissolved in 50ml of anhydrous ether; 1.50g of EGF and the above vitamin E acetate-cyclodextrin inclusion compound 3.00g were dissolved in 50ml of 0.02M pH7. 4 in phosphate buffered saline. After the solution was fully mixed, it was subjected to ultrasonic treatment in an ice-water bath (ultrasonic power 80%, ultrasonic time 5 min), and the...

Embodiment 2

[0039] (1) Weigh 3.80g of hydroxypropyl-β-cyclodextrin, add 100ml of distilled water to fully dissolve it, and take another 0.70g of vitamin E acetate, stir at 30°C for 4h, and dissolve the dispersion of the clathrate in Centrifuge at 70,000g for 60min, the supernatant is an aqueous solution of vitamin E acetate-cyclodextrin inclusion compound, concentrate the supernatant in vacuum, and dry the concentrate in a vacuum drying oven at 40°C and 0.08MPa for 6h to obtain powdered vitamin E acetate-cyclodextrin inclusion compound, the inclusion rate reaches 82.53% through measurement.

[0040] (2) Mix 10.25g egg yolk lecithin and 2.50g cholesterol and dissolve in 50ml of anhydrous ether; dissolve 1.50g of EGF and the above vitamin E acetate-cyclodextrin inclusion complex 3.00g in 50ml of 0.02M pH7.4 in phosphate buffered saline solution. After the solution was fully mixed, it was subjected to ultrasonic treatment in an ice-water bath (ultrasonic power 80%, ultrasonic time 5 min), a...

Embodiment 3

[0043] (1) Weigh 4.50g of β-cyclodextrin, add 100ml of distilled water to fully dissolve it, take another 0.90g of vitamin E acetate, stir at 50°C for 5h, and centrifuge the dispersion of inclusion compound at 70000g for 60min , the supernatant is an aqueous solution of vitamin E acetate-cyclodextrin inclusion compound, the supernatant is concentrated in vacuum, and the concentrate is dried in a vacuum oven at 40°C and 0.08MPa for 6 hours to obtain powdered vitamin E acetate -Cyclodextrin inclusion compound, the inclusion rate reaches 86.23% through measurement.

[0044] (2) 10.25g of hydrogenated soybean lecithin and 2.50g of cholesterol were mixed and dissolved in 50ml of anhydrous ether; 1.50g of EGF and the above vitamin E acetate-cyclodextrin inclusion compound 3.00g were dissolved in 50ml of 0.02M pH7.4 in phosphate buffered saline solution. After the solution was fully mixed, it was subjected to ultrasonic treatment in an ice-water bath (ultrasonic power 80%, ultrasoni...

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Abstract

The invention relates to an EGF / vitamin E acetate-cyclodextrin liposome. The EGF / vitamin E acetate-cyclodextrin liposome comprises the following raw materials in parts by weight: 2-50 parts of an EGFstock solution, 2-50 parts of vitamin E acetate, 30-100 parts of cyclodextrin or a cyclodextrin derivative, 100-220 parts of a phospholipid material, 20-70 parts of cholesterol and 40-155 parts of a freeze-drying protective agent. The invention also provides a preparation method of the EGF / vitamin E acetate-cyclodextrin liposome. The invention also provides a use method of the EGF / vitamin E acetate-cyclodextrin liposome. The invention also provides application of the EGF / vitamin E acetate-cyclodextrin liposome in cosmetics. The EGF / vitamin E acetate-cyclodextrin liposome provided by the invention has the following beneficial effects: EGF and vitamin E acetate are encapsulated into a liposome, so that the stability and bioavailability of the EGF and vitamin E acetate are improved; and meanwhile, the EGF and vitamin E acetate can act synergistically to achieve anti-aging and repairing effects on skin together, so as to achieve better skin care results.

Description

technical field [0001] The present invention relates to a kind of EGF / vitamin E acetate-cyclodextrin liposome, also relate to a kind of preparation method of EGF / vitamin E acetate-cyclodextrin liposome, also relate to a kind of EGF / vitamin E The use method and application of acetate-cyclodextrin liposome. Background technique [0002] The Chinese name of EGF (Epidermal Growth Factoy) is epidermal growth factor, which consists of 53 amino acids (and includes 3 disulfide bonded polypeptides). Professors Montalcini and Cohen were awarded the Nobel Prize in Physiology and Medicine in 1986 for their discovery of epidermal growth factor and their analysis of its structure and mechanism of action. EGF can stimulate the tyrosine phosphorylation of epidermal growth factor receptor to achieve the effect of repair and hyperplasia, so as to replace aging and dead cells with new cells, and fundamentally achieve rapid repair of skin damage, repair of wrinkles and wrinkle removal , Sensi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/14A61K8/73A61K8/67A61K8/64A61K8/55A61Q19/08A61Q19/00
CPCA61K8/14A61K8/553A61K8/64A61K8/678A61K8/738A61K2800/10A61K2800/592A61K2800/84A61Q19/00A61Q19/08
Inventor 柴营营黄赫
Owner YANTAI KANGDAER PHARMA CO LTD
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