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Composite bacterium preparation, preparation method thereof, and drilling well wastewater processing method

A technology of drilling wastewater and complex bacteria, which is applied in the field of microorganisms, can solve the problems of difficult removal of organic matter, etc., and achieve the effects of long validity period, good application prospects and good degradation effect

Inactive Publication Date: 2018-03-13
SINOPEC SSC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of this, the present invention aims at the technical defects of difficult removal of organic matter in drilling wastewater and the need for secondary treatment in the prior art, and provides a compound bacterial preparation with fast degradation speed, good degradation effect and strong adaptability and its preparation method and application

Method used

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  • Composite bacterium preparation, preparation method thereof, and drilling well wastewater processing method
  • Composite bacterium preparation, preparation method thereof, and drilling well wastewater processing method
  • Composite bacterium preparation, preparation method thereof, and drilling well wastewater processing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The separation and purification of embodiment 1 Micrococcus klebsi ZL-01

[0041] 1. Separation and purification

[0042] Taking the petroleum-contaminated soil of the oilfield well site as the sampling source, the bacteria in the soil were enriched and cultivated, and then spread on the solid medium containing 3% high molecular weight organic compound guar gum, and cultured at 40°C for 7 days, and then in the A single colony was picked from the plate, separated and purified by streaking on the plate, and a strain with excellent traits was obtained, which was coded as YL-01.

[0043] 2. Identification of strains

[0044] (1) Culture characteristics

[0045] Micrococcus klebsi YL-01 was inoculated on the guar agar medium, and orange colonies grew after 2 days of culture. The cells were nearly spherical and appeared in pairs. Oxygen bacteria, the growth temperature is 33 ~ 37 ℃.

[0046] (2) Using 16S rRNA to identify the strain

[0047] According to the method descr...

Embodiment 2

[0058] Embodiment 2: the preparation of a kind of composite bacteria preparation

[0059] 1. Experimental materials

[0060] Seed medium: Each 1L contains 10g peptone, 5g yeast extract, 1g glucose, 1g sodium chloride, 1g potassium dihydrogen phosphate, 0.5g dipotassium hydrogen phosphate, supplemented with water, and sodium hydroxide to adjust the pH of the medium to 6.

[0061] Fermentation medium: every 1L contains 1g of yeast extract, 0.5g of ammonium nitrate, 0.01g of sodium chloride, 0.05g of dipotassium hydrogen phosphate, 0.05g of potassium dihydrogen phosphate, 0.1g of magnesium sulfate, 0.1g of Calcium chloride and water were supplemented, and sodium hydroxide was used to adjust the pH of the medium to 6.

[0062] 2. Preparation

[0063] (1) Micrococcus klebsi YL-01, Bacillus subtilis ZJ-02 and Candida lipolytica ZJ-03 were respectively inoculated in the seed medium with an inoculum size of 5% by volume, on a shaker at 25°C Cultivate at 100 rpm for 24 hours to obta...

Embodiment 3

[0067] Embodiment 3: a kind of preparation method of composite bacteria preparation

[0068] 1. Experimental materials

[0069] Each 1L of the seed medium contains 15g peptone, 10g yeast extract, 2g glucose, 5g sodium chloride, 3g potassium dihydrogen phosphate, 1.5g dipotassium hydrogen phosphate, supplemented with water, and sodium hydroxide to adjust the pH of the medium to 8.

[0070] Each 1L of fermentation medium contains 2g of yeast extract, 2g of ammonium nitrate, 0.2g of sodium chloride, 2g of dipotassium hydrogen phosphate, 1g of potassium dihydrogen phosphate, 0.2g of magnesium sulfate, 0.5g of calcium chloride, Water was supplemented, and sodium hydroxide was used to adjust the pH of the medium to 8.

[0071] 2. Preparation

[0072] (1) Micrococcus klebsi YL-01, Bacillus subtilis ZJ-02 and Candida lipolytica ZJ-03 were respectively inoculated in the seed medium with an inoculum size of 10% by volume, and placed on a shaker at 37°C Cultivate at 200 rpm for 48 hou...

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Abstract

The invention belongs to the field of microbial preparation, and specifically relates to a composite bacterium preparation, a preparation method thereof, and a drilling well wastewater processing method. The composite bacterium preparation comprises following components in percentage by dry weight: 25 to 50 % of micrococcus kristinae YL-01, 15 to 35% of bacillus subtilis ZJ-02, and 20 to 40% of candida lipolytica ZJ-03; and the number of live bacteria are all 1010 to 1012 for each gram. The invention also discloses a preparation method of the composite bacterium preparation, and a method of utilizing the composite bacterium preparation to process drilling well wastewater. The experiment results show that the composite bacterium preparation has a high degradation rate on COD in drilling well wastewater, the degradation effect is good, no secondary pollution is generated during the processing process, the sludge does not need processed for another time, and the composite bacterium preparation has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a compound bacteria preparation, a preparation method thereof and a treatment method for drilling wastewater. Background technique [0002] In the process of oil extraction, a large amount of drilling wastewater will be produced. The wastewater mainly comes from discarded and scattered mud, power water for flushing equipment, wastewater generated during testing and operations during drilling, and natural rainfall. It contains a large amount of sediment, oily substances, heavy metal pollutants and various chemical treatment agents. If the drilling wastewater is directly discharged without treatment, it will have a great impact on the surrounding ecological environment. Therefore, it is of great significance to study the treatment methods of drilling wastewater and reduce the harm of drilling wastewater. [0003] The composition of oilfield drilling wastewater...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C02F3/34C12R1/125C12R1/73C12R1/265C02F103/10
CPCC02F3/347C02F2103/10C12N1/16C12N1/20
Inventor 单海霞何焕杰马雅雅徐勤詹适新贾深
Owner SINOPEC SSC
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