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Preparation method of electrochemical immunosensor for detecting tumor marker-loaded molecular sieve

A tumor marker and immunosensor technology is applied in the field of preparation of a molecular sieve-loaded electrochemical immunosensor for detecting tumor markers, and achieves the effects of good electrical conductivity, large surface area, and high catalytic performance.

Inactive Publication Date: 2018-03-27
NINGBO COLLEGE OF HEALTH SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no published reports at home and abroad about sandwich-type electrochemical luminescence immunosensors for detecting tumor markers based on graphene oxide and carbon nitride nanocomposites.

Method used

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Embodiment 1

[0050] A preparation method for detecting tumor marker-loaded molecular sieve electrochemical immunosensor, comprising the following steps:

[0051] Step (1), the glassy carbon electrode with a diameter of 4mm is polished with Al2O3 polishing powder, and cleaned with ultrapure water;

[0052] Take 8 μL, 1.5 mg / mL chitosan solution of piperazine-modified aminated graphene and add it dropwise to the electrode surface, dry it at room temperature, rinse the electrode surface with ultrapure water, and dry it; add 8 μL dropwise at a concentration of 0.8 mol / L 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide EDC and 0.3mol / L N-hydroxysuccinimide NHS solution to the electrode surface, incubate at 4°C for 40min , dry at room temperature;

[0053] Step (2), add 8 μL, 10 μg / mL primary tumor marker antibody dropwise on the surface of the working electrode modified in step (1), rinse the electrode surface with ultrapure water, and dry it in a refrigerator at 4°C;

[0054] Step (3), drop 8 ...

Embodiment 2

[0087] A preparation method for detecting tumor marker-loaded molecular sieve electrochemical immunosensor, comprising the following steps:

[0088] Step (1), the glassy carbon electrode with a diameter of 4mm is polished with Al2O3 polishing powder, and cleaned with ultrapure water;

[0089] Take 8 μL, 2.5 mg / mL chitosan solution of piperazine-modified aminated graphene and add it dropwise to the electrode surface, dry it at room temperature, rinse the electrode surface with ultrapure water, and dry it; add 8 μL dropwise at a concentration of 1.0 mol / L 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide EDC and 0.4mol / L N-hydroxysuccinimide NHS solution to the electrode surface, incubate at 4°C for 40min , dry at room temperature;

[0090] Step (2), add 8 μL, 12 μg / mL primary tumor marker antibody dropwise on the surface of the working electrode modified in step (1), rinse the electrode surface with ultrapure water, and dry it in a refrigerator at 4°C;

[0091] Step (3), drop 8 ...

Embodiment 3

[0124] A preparation method for detecting tumor marker-loaded molecular sieve electrochemical immunosensor, comprising the following steps:

[0125] Step (1), the glassy carbon electrode with a diameter of 4mm is polished with Al2O3 polishing powder, and cleaned with ultrapure water;

[0126] Take 8 μL, 2.0 mg / mL chitosan solution of piperazine-modified aminated graphene and add it dropwise to the electrode surface, dry it at room temperature, rinse the electrode surface with ultrapure water, and dry it; add 8 μL dropwise at a concentration of 0.9 mol / L 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide EDC and 0.35mol / L N-hydroxysuccinimide NHS solution to the electrode surface, incubate at 4°C for 40min , dry at room temperature;

[0127] Step (2), add 8 μL, 11 μg / mL primary tumor marker antibody dropwise on the surface of the working electrode modified in step (1), rinse the electrode surface with ultrapure water, and dry it in a refrigerator at 4°C;

[0128] Step (3), drop 8...

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Abstract

The invention provides a preparation method of an electrochemical immunosensor for detecting a tumor marker-loaded molecular sieve. The preparation method comprises the following steps: polishing a glassy carbon electrode with Al2O3 polishing powder, and cleaning with ultra-pure water; dropwise adding a chitosan solution of piperazine-modified aminated graphene onto the surface of the electrode, rinsing and airing; dropwise adding 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N-hydroxysuccinimide (NHS) solutions onto the surface of the electrode; coating the surface of a working electrode with a silver nanoparticle p-thiophenol functional sulfonated carbon nanotube in a dropping manner to detect an antibody Ab2 incubation solution; dropwise adding a SAPO-34-Pd / Co-Ab2 second antibody incubation solution to prepare the electrochemical immunosensor for detecting the tumor marker-loaded molecular sieve. By adopting the piperazine-modified aminated graphene, a relatively large surface area, higher conductivity, high biocompatibility and high stability of the graphene are kept.

Description

technical field [0001] The invention belongs to the technical fields of novel functional nanomaterials, immune analysis and biosensing, and provides a preparation method of an electrochemical immunosensor loaded with molecular sieves for detecting tumor markers. Background technique [0002] Tumor is the killer of human health. Malignant tumors, also known as cancer, are a type of disease that seriously threatens human health and life. Under the action of various internal or external carcinogenic factors, cells in local tissues lose their ability to grow at the genetic level. Normal regulation, resulting in the formation of new organisms due to abnormal proliferation. According to WHO research, in 2012, the number of cancer patients and deaths in the world increased disturbingly. Nearly half of the new cancer cases occurred in Asia, most of which were in China. The number of new cancer cases in China ranked first in the world. WHO also pointed out that early diagnosis and ...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/3275
Inventor 凌庆枝马少华秦志伟卢金
Owner NINGBO COLLEGE OF HEALTH SCI
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