Preparation method of electrochemical immunosensor for detecting tumor marker-loaded molecular sieve
A tumor marker and immunosensor technology is applied in the field of preparation of a molecular sieve-loaded electrochemical immunosensor for detecting tumor markers, and achieves the effects of good electrical conductivity, large surface area, and high catalytic performance.
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Embodiment 1
[0050] A preparation method for detecting tumor marker-loaded molecular sieve electrochemical immunosensor, comprising the following steps:
[0051] Step (1), the glassy carbon electrode with a diameter of 4mm is polished with Al2O3 polishing powder, and cleaned with ultrapure water;
[0052] Take 8 μL, 1.5 mg / mL chitosan solution of piperazine-modified aminated graphene and add it dropwise to the electrode surface, dry it at room temperature, rinse the electrode surface with ultrapure water, and dry it; add 8 μL dropwise at a concentration of 0.8 mol / L 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide EDC and 0.3mol / L N-hydroxysuccinimide NHS solution to the electrode surface, incubate at 4°C for 40min , dry at room temperature;
[0053] Step (2), add 8 μL, 10 μg / mL primary tumor marker antibody dropwise on the surface of the working electrode modified in step (1), rinse the electrode surface with ultrapure water, and dry it in a refrigerator at 4°C;
[0054] Step (3), drop 8 ...
Embodiment 2
[0087] A preparation method for detecting tumor marker-loaded molecular sieve electrochemical immunosensor, comprising the following steps:
[0088] Step (1), the glassy carbon electrode with a diameter of 4mm is polished with Al2O3 polishing powder, and cleaned with ultrapure water;
[0089] Take 8 μL, 2.5 mg / mL chitosan solution of piperazine-modified aminated graphene and add it dropwise to the electrode surface, dry it at room temperature, rinse the electrode surface with ultrapure water, and dry it; add 8 μL dropwise at a concentration of 1.0 mol / L 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide EDC and 0.4mol / L N-hydroxysuccinimide NHS solution to the electrode surface, incubate at 4°C for 40min , dry at room temperature;
[0090] Step (2), add 8 μL, 12 μg / mL primary tumor marker antibody dropwise on the surface of the working electrode modified in step (1), rinse the electrode surface with ultrapure water, and dry it in a refrigerator at 4°C;
[0091] Step (3), drop 8 ...
Embodiment 3
[0124] A preparation method for detecting tumor marker-loaded molecular sieve electrochemical immunosensor, comprising the following steps:
[0125] Step (1), the glassy carbon electrode with a diameter of 4mm is polished with Al2O3 polishing powder, and cleaned with ultrapure water;
[0126] Take 8 μL, 2.0 mg / mL chitosan solution of piperazine-modified aminated graphene and add it dropwise to the electrode surface, dry it at room temperature, rinse the electrode surface with ultrapure water, and dry it; add 8 μL dropwise at a concentration of 0.9 mol / L 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide EDC and 0.35mol / L N-hydroxysuccinimide NHS solution to the electrode surface, incubate at 4°C for 40min , dry at room temperature;
[0127] Step (2), add 8 μL, 11 μg / mL primary tumor marker antibody dropwise on the surface of the working electrode modified in step (1), rinse the electrode surface with ultrapure water, and dry it in a refrigerator at 4°C;
[0128] Step (3), drop 8...
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Abstract
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