High-throughput liquid chromatography tandem mass spectrometry detection method with high-throughput liquid chromatography tandem mass spectrometry

Abstract

The invention discloses a high-throughput liquid chromatography tandem mass spectrometry detection method with high-throughput liquid chromatography tandem mass spectrometry, wherein the method includes the steps of S1 sample preparation, S2 sample pretreatment, S3 liquid chromatography separation, S4 tandem mass spectrometry detection and the like. In addition, the invention also discloses the method for detecting 15 kinds of bile acids by the high-throughput liquid chromatography tandem mass spectrometry method. The method provides the high-throughput liquid chromatography tandem mass spectrometry detection method, wherein the detection method is suitable for detection of the bile acids in human serum, has simple sample pretreatment, can simultaneously detect 15 kinds of bile acids, andhas the advantages of good detection specificity, short whole detection process time and high throughput.

Description

technical field [0001] The invention relates to the technical field of bile acid detection, in particular to a high-throughput liquid chromatography tandem mass spectrometry detection method. Background technique [0002] Bile acid, the main organic component of bile, is a general term for a class of 24-carbon cholic acid hydroxyl derivatives. Bile acids can be divided into two types according to their structure: free bile acid and conjugated bile acid. According to their production sites and structures, they are divided into primary and secondary bile acids. Primary bile acids are converted from intrahepatic cholesterol through a series of reactions to finally generate cholic acid (CA), chenodeoxycholic aic (CDCA), and conjugated bile acids formed with glycine and taurine , including glycocholic acid (GCA), glycochenodeoxycholic acid (GCDCA), taurocholic acid (TCA) and taurochenodeoxycholic acid (TCDCA). After the primary bile acid is synthesized in the liver cells, a se...

AI Technical Summary

Problems solved by technology

At present, the conventional high-throughput liquid chromatography tandem mass spectrometry detection method is the cyclic enzymatic method to measure bile acids in physiological solutions. Although the operation is relatively simple, this method can only detect the total bile acid content, and cannot measure the difference of different bile acids. ; Gas chromatography and gas chromatography-mass chromatography require complex sample preparation and derivatization when detecting bile acids, and it is necessary to hydrolyze the bound bile acids into unbound forms for analysis; HPLC series UV detectors are used in the detection of complex biological matrices bile acids can lead to insufficient sensitivity and specificity; fluorescence detection method requires complex derivatization techniques and cannot be generalized

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