Method for detecting pulmonary embolism

A technology for pulmonary embolism and sample detection, applied in the field of biomedicine, can solve the problems of elevated D-dimer and low specificity, and achieve the effect of simple operation, mature technology and wide industrial value

Inactive Publication Date: 2018-03-30
傅应云
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Problems solved by technology

Although D-dimer has high sensitivity, many diseases in the body (such as cardiovascular disease, surgery, tumor, infection and tissue necrosis, etc.) will lead to increased D-dimer, so its specificity is not high. If the result is positive, further CTPA examination is required

Method used

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  • Method for detecting pulmonary embolism
  • Method for detecting pulmonary embolism
  • Method for detecting pulmonary embolism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0050] The test materials are as follows:

[0051] Standard microspheres (Megamix plus SSC beads): French BioCytex company.

[0052] Flow antibody: American BD company.

[0053] Absolute counting tube: American BD company.

[0054] Flow cytometer: BD Company, USA.

[0055] Elisa kit: American R&D Company.

[0056] Dichotomous stepwise logistic regression analysis: MedCalc software, Maria Kerke, Belgium, version 11.4.2.0.

[0057] 1. PMPs flow cytometry antibody labeling

[0058] The method of PMPs flow cytometry antibody labeling is as follows: Standard flow method is used for labeling.

[0059] The specific steps are: collect 2 mL of peripheral venous blood from the research subjects, centrifuge the blood for 15 minutes under a centrifugal force of 2500 g, separate the plasma, and centrifuge the separated plasma again for 15 minutes to obtain platelet-poor plasma (PFP) samples.

[0060] Dilute 10X annexin V binding buffer to 1X working solution, such as: 1mL Buffer+9mL ...

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Abstract

The present invention provides methods for detecting pulmonary embolism. The method is based on the absolute count of platelet microparticles in plasma measured by standard flow cytometry as an adjunct to the diagnosis of pulmonary embolism. Through ROC curve analysis, the critical value of platelet microparticles for diagnosing pulmonary embolism was 716.90/μl, and the sensitivity and specificity at this critical value were 58.82% and 95.92%, respectively. The positive likelihood ratio was 14.41, and the negative likelihood ratio was 0.43. The positive predictive value was 93.75%, the negative predictive value was 69.12%, and the Youden's index was 54.74%. This method is a non-invasive operation, low cost, easy to carry out, and high specificity. It can further screen patients with positive D-dimer results in clinical practice, improve diagnostic specificity, and can be used as CTPA for patients with pulmonary embolism. The pre-examination evaluation method can reduce the adverse reactions caused by CTPA examination and reduce the burden on patients.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for detecting pulmonary embolism. Background technique [0002] The incidence of pulmonary embolism (PE) occupies the third place among cardiovascular diseases. Due to the lack of specificity in the clinical manifestations of PE, about 80% of PTE are missed and misdiagnosed, resulting in PE becoming a human with high morbidity and mortality. major health problems. [0003] Studies have shown that clear and early diagnosis and appropriate treatment can reduce the mortality rate of PE to 2% to 8%. At present, the diagnosis of PTE mainly relies on CT pulmonary angiography to find the thrombus filling defect in the pulmonary artery. However, only 5% to 10% of suspected patients who underwent CTPA examination were true PE patients. In addition, CTPA examination is expensive and cannot be detected at any time, which delays the diagnosis of acute pulmonary embolism, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14G01N15/10G01N15/00
CPCG01N15/14G01N15/00G01N15/10G01N15/1429G01N2015/0084G01N2015/1006G01N2015/1087G01N2015/1402G01N2015/1486G01N2015/1493
Inventor 傅应云王敏连许兰肖露乐雍建刘盛国黄启俊李树林
Owner 傅应云
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