Method for preparing pickled papaya brewed wine from brown sugar and pickled papaya
A technology of sour papaya and fermented wine, which is applied in microorganism-based methods, biochemical equipment and methods, and preparation of alcoholic beverages, etc., to achieve the effects of stable strain characteristics, convenient large-scale industrial production, and abundant raw material sources.
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Embodiment 1
[0029] (1) The Pichia kurdori YNCA 9066 strain (CCTCC M 2017377) stored on a solid slant was transferred to the liquid seed medium and cultivated on a shaker at 32°C for 24 h at a speed of 180r / min.
[0030] Slope solid medium: glucose 20 g / L, yeast extract 10 g / L, peptone 20 g / L, agar 20 g / L, pH 6.5, sterilized at 121°C for 25 minutes, cooled to 60°C and poured onto a plate for later use.
[0031] Liquid seed medium: glucose 20 g / L, yeast extract 10 g / L, peptone 20 g / L, add distilled water to 1000 mL, pH 6.5, put into 250 mL Erlenmeyer flask, 100 mL / bottle, 121 ° C, 25 min Bacteria spare;
[0032] (2) Select 8-9 fresh sour papayas that are ripe, free from diseases and insect pests, full of fruit, and free of rot, and beat them into a homogenate with a blender after cleaning. Brown sugar is ground into powder with a mixer.
[0033] (3) Put 10 bottles of Pichia kurdori YNCA 9066 seed solution grown in step (1) into a 30L fermenter at 5% (v / v), add 1kg of sour papaya juice pre...
Embodiment 2
[0038] Repeat Example 1, with the following differences:
[0039] (3) Add 2kg sour papaya juice and 4kg brown sugar powder.
[0040] (5) The fermented sour papaya wine is slightly sour than the product of Example 1.
Embodiment 3
[0042] Repeat Example 1, with the following differences:
[0043] (3) Add 1kg sour papaya juice and 2kg brown sugar powder.
[0044](4) Culture control conditions: fermentation time 84 hours, alcohol content reaches 12 degrees
[0045] (5) The fermented sour papaya wine is slightly sour than the product of Example 1, and the fragrance is lighter.
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