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Crystal-violet salmonella culture medium and preparation process thereof

A Salmonella culture medium and production process technology, which can be used in the determination/inspection of microorganisms, biochemical equipment and methods, resistance to vector-borne diseases, etc. The judgment of the results of scholars is quite difficult, and the detection time of Salmonella is long.

Inactive Publication Date: 2018-04-17
宁波市纯韩花漫生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the selectivity and specificity of the traditional culture medium for detecting Salmonella at present cannot reach satisfactory results, (the formula of the traditional culture medium has the following several: 1. buffered peptone water enrichment, formula: 10 grams of peptone, sodium chloride 5 grams, 9 grams of disodium hydrogen phosphate, 1.5 grams of potassium dihydrogen phosphate; 1g of bile salt, 7g of calcium carbonate, 0.01g of brilliant green, 20ml of 0.1mol / L iodine solution; and GN enrichment solution, formula: 20g of tryptone, 1g of glucose, 2g of mannitol, citric acid 5 grams of sodium, 0.5 grams of sodium deoxycholate, 4 grams of dipotassium hydrogen phosphate, 1.5 grams of potassium dihydrogen phosphate, and 5 grams of sodium chloride; 3, transfer to HE agar plate for separation, formula: 12 grams of peptone, 3 grams of beef extract gram, lactose 12 grams, sucrose 12 grams, salicin 2 grams, bile salt 20 grams, sodium chloride 5 grams, agar 15 grams, bromothymol blue 0.064 grams, acid fuchsin 0.1 grams, sodium thiosulfate 6.8 grams, iron citrate press 0.8 grams, sodium deoxycholate 2 grams
) The detection time of Salmonella is relatively long and the resolution is low, which can only be judged by experience, and it is quite difficult for beginners to judge the results

Method used

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  • Crystal-violet salmonella culture medium and preparation process thereof
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  • Crystal-violet salmonella culture medium and preparation process thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0014] A kind of Salmonella crystalline purple culture medium of the present embodiment, the weight portion of described raw material is:

[0015] Peptone 10g; lactose 10g; L-cysteine ​​0.5g; beef powder 5g; sodium chloride 5g; sodium citrate 5g; bile salt 10g; sodium thiosulfate 5g; crystal violet 0.005g; ferric ammonium citrate 1g; Bromothymol blue 0.064g; agar 12g. The production process of the culture medium in this example includes the following steps: First, weigh all the raw materials except agar according to the above ratio, mix them evenly, put them in distilled water, heat them with an electric furnace until they dissolve, and keep stirring; after they are completely dissolved, cool them down naturally To 25-30°C, use sodium carbonate to adjust the pH value to 7.2-7.4, then add the agar of the above ratio for three times of boiling sterilization, after sterilization, the pH value reaches 7.2-7.4; when it is cooled to about 55°C, pour 90mm of clean Petri dish, 15-20m...

Embodiment 2

[0018] A kind of Salmonella crystalline purple culture medium of the present embodiment, the weight portion of described raw material is:

[0019] Peptone 5g; lactose 5g; L-cysteine ​​0.1g; beef powder 1g; sodium chloride 1g; sodium citrate 1g; bile salt 5g; sodium thiosulfate 1g; crystal violet 0.001g; ferric ammonium citrate 0.5g ; Bromothymol blue 0.050g; Agar 11g. The production process of the culture medium in this example includes the following steps: First, weigh all the raw materials except agar according to the above ratio, mix them evenly, put them in distilled water, heat them with an electric furnace until they dissolve, and keep stirring; after they are completely dissolved, cool them down naturally To 25-30°C, use sodium carbonate to adjust the pH value to 7.2-7.4, then add the agar of the above ratio for three times of boiling sterilization, after sterilization, the pH value reaches 7.2-7.4; when it is cooled to about 55°C, pour 90mm of clean Petri dish, 15-20m...

Embodiment 3

[0022] A kind of Salmonella crystalline purple culture medium of the present embodiment, the weight portion of described raw material is:

[0023] Peptone 15g; lactose 15g; L-cysteine ​​1g; beef powder 9g; sodium chloride 9g; sodium citrate 9g; bile salt 15g; sodium thiosulfate 9g; crystal violet 0.01g; ferric ammonium citrate 1.5g; Bromothymol blue 0.070g; agar 18g. The production process of the culture medium in this example includes the following steps: First, weigh all the raw materials except agar according to the above ratio, mix them evenly, put them in distilled water, heat them with an electric furnace until they dissolve, and keep stirring; after they are completely dissolved, cool them down naturally To 25-30°C, use sodium carbonate to adjust the pH value to 7.2-7.4, then add the agar of the above ratio for three times of boiling sterilization, after sterilization, the pH value reaches 7.2-7.4; when it is cooled to about 55°C, pour 90mm of clean Petri dish, 15-20ml...

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Abstract

The invention discloses a crystal-violet salmonella culture medium and a preparation process thereof. The culture medium is prepared from raw materials in parts by weight as follows: 5-15 parts of peptone, 5-15 parts of lactose, 0.1-1 part of L-cysteine, 1-9 parts of beef powder, 1-9 parts of sodium chloride, 1-9 parts of sodium citrate, 5-15 parts of cholate, 1-9 parts of sodium thiosulfate, 0.001-0.01 parts of crystal violet, 0.5-1.5 parts of ammonium ferric citrate, 0.050-0.070 parts of bromothymol blue and 11-18 parts of agar. The operation is simple and convenient, medium types required in detection can be reduced, detection time is shortened to 2 d from 4-7 d in the conventional method, and the detection sensitivity is high.

Description

technical field [0001] The invention relates to a culture medium for Salmonella crystalline purple and a production process thereof. Background technique [0002] Culture medium refers to the artificially prepared nutrient matrix suitable for the growth and reproduction of bacteria, which can be used for the cultivation, separation, research and preservation of microorganisms. , Agricultural microbial tissue culture, industrial microbial inspection, such as food inspection, pharmaceutical biological inspection, cosmetic inspection, drinking water inspection, disposable hygiene product inspection and many other industries to test bacteria, have a wide range of applications and progress, and are closely related to human life. [0003] Salmonellosis is one of the zoonotic diseases of great significance in public health, including those bacteria that cause food poisoning, gastroenteritis, typhoid fever and paratyphoid fever. The traditional culture and detection time takes 4-7 d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/10
CPCC12Q1/10Y02A50/30
Inventor 张永超
Owner 宁波市纯韩花漫生物科技有限公司
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