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Hydrolyzed sericin peptide preparation method

A sericin peptide and hydrolysis technology, which is applied in the field of protein separation and purification, can solve the problems of low degumming efficiency, difficult application and good results, and difficult separation of phosphate.

Active Publication Date: 2018-04-20
YIBIN PINGSHAN HUIRUI OIL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of this method are: using high-concentration phosphoric acid, the price is high; neutralizing phosphoric acid requires a large amount of calcium salt, etc., generating a large amount of low-value-added phosphate and difficult to separate completely, which seriously affects product quality; the prepared tussah silk peptide has not been processed Hydrolysis, large molecular weight, difficult to store stably
The defect of this invention is that the degumming efficiency is extremely low. In addition, without fractional separation, what is obtained is a mixture of sericins with different molecular weights. Due to the inhomogeneous molecular weight, it is difficult to apply in a specific environment and achieve good results.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Prepare and clean silkworm cocoon shells

[0035] First remove the sundries such as branches and leaves, silkworm chrysalis and stones on the silkworm cocoon shell manually, and then according to the volume ratio (kg / L) of the sodium carbonate aqueous solution of 0.2% of the mass percentage concentration of the silkworm cocoon shell is 1: 20. Ratio, the artificial impurity-removed silkworm cocoon shells were dispersed in an aqueous sodium carbonate solution with a concentration of 0.2% by mass, and stirred at 60° C. for 30 minutes. Filter and collect the filter residue and filtrate respectively. The collected filtrate is pumped into the waste water tank for biochemical treatment, and discharged after reaching the standard; the collected filter residue is prepared to clean silkworm cocoon shells, which are used in the next step to prepare a desericin solution.

[0036] (2) prepare sericin solution

[0037] After the (1) step is completed, first according to the rat...

Embodiment 2

[0055] (1) Prepare and clean silkworm cocoon shells

[0056] First remove the sundries such as branches and leaves, silkworm chrysalis and stones on the silkworm cocoon shell manually, and then according to the volume ratio (kg / L) of the sodium carbonate aqueous solution (kg / L) of 0.4% of the mass percentage concentration of the silkworm cocoon shell is 1: 30. Ratio, the artificial impurity-removed silkworm cocoon shells were dispersed in an aqueous sodium carbonate solution with a concentration of 0.4% by mass, and stirred at 70° C. for 35 minutes. Filter and collect the filter residue and filtrate respectively. The collected filtrate is pumped into the waste water tank for biochemical treatment, and discharged after reaching the standard; the collected filter residue is prepared to clean silkworm cocoon shells, which are used in the next step to prepare a desericin solution.

[0057] (2) prepare sericin solution

[0058] After the (1) step was completed, earlier according ...

Embodiment 3

[0076] (1) Prepare and clean silkworm cocoon shells

[0077] First remove the sundries such as branches and leaves, silkworm chrysalis and stones on the silkworm cocoon shell manually, and then according to the volume ratio (kg / L) of the sodium carbonate aqueous solution of 0.5% concentration of the silkworm cocoon shell and the mass percentage concentration is 1: 40 Ratio, the artificial impurity-removed silkworm cocoon shells were dispersed in an aqueous sodium carbonate solution with a concentration of 0.5% by mass, and stirred at 80° C. for 40 minutes. Filter and collect the filter residue and filtrate respectively. The collected filtrate is pumped into the waste water tank for biochemical treatment, and discharged after reaching the standard; the collected filter residue is prepared to clean silkworm cocoon shells, which are used in the next step to prepare a desericin solution.

[0078] (2) prepare sericin solution

[0079] After the (1) step was completed, earlier acc...

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Abstract

The invention relates to a hydrolyzed sericin peptide preparation method, and belongs to the technical field of protein separation and purification. According to the present invention, tasar silkwormcocoon shell or waste mulberry silk is used as a raw material, the tasar silkworm cocoon shell is washed, a sericin solution is prepared, a hydrolyzed sericin peptide solution is prepared, an activated macroporous resin decolorization system is assembled, a hydrolyzed sericin peptide decolorization solution is prepared, an activated MCI GEL resin system is assembled, a hydrolyzed sericin peptide grading separation solution is prepared, a hydrolyzed sericin peptide grading separation concentration solution is prepared, a hydrolyzed sericin peptide grading separation ethanol removing concentration solution is prepared, and hydrolyzed sericin peptide freeze-drying powder is prepared so as to obtain the product; by using multi-enzyme combined hydrolysis, ultra-filtration, nano-filtration separation, macroporous resin decolorization, MCI GEL resin grading separation, low-temperature concentration, freeze drying, and other modern chemical industry technologies and equipment, such that the preparation method has characteristics of convenient operation, mild condition, high efficiency, energy saving, low production cost and the like; and the product prepared by using the preparation methodcan be widely used in the fields of nutritious daily chemicals, fiber modification, and the like.

Description

1. Technical field [0001] The invention belongs to the technical field of protein separation and purification, and in particular relates to a preparation method of hydrolyzed sericin peptide. 2. Background technology [0002] Silk is mainly composed of 20-30% sericin and 70-80% silk fibroin, and is the raw material for making silk. In addition, its components, sericin and silk fibroin, are also used in industries such as medical care, health care, daily chemicals, textiles, food, building materials, etc., to play their roles in blood coagulation, bone setting, water retention, whitening, antibacterial, anti-ultraviolet, packaging and coatings. The field and consumption are expanding year by year. It is a natural resource with huge market potential and is worth developing and utilizing. [0003] At present, silk sericin, silk fibroin and their deep-processed products are all prepared from waste silk or cocoon shells. There are existing methods for preparing silk sericin pep...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34C07K1/20C07K1/16C07K1/14
CPCC12P21/06C07K1/14C07K1/16C07K1/20C07K1/34
Inventor 孙益华刘志军
Owner YIBIN PINGSHAN HUIRUI OIL CO LTD
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