Novel Lepidium meyenii Walp. alkaloid, and preparation method and application thereof

Abstract

The invention discloses a novel Lepidium meyenii Walp. alkaloid, and a preparation method and application thereof. The structural formula of the alkaloid is shown in the description, and the alkaloidis a novel hexahydroimidazo[1,5-c]thiazole alkaloid with a formula of C14H14N2O3S2, and is named as (+)-meyeniin A. The preparation method takes rhizome of a cruciferae lepidium plant Lepidium meyeniiWalp. as a raw material, and comprises the steps of organic solvent extraction, liquid-liquid extraction, MCI impurity removal, silica gel column chromatography, high-pressure liquid chromatography and other extraction separation steps for obtaining the alkaloid. The application means application of the alkaloid to anticancer drugs, functional food and health care products. The (+)-meyeniin A shows a certain inhibitory effect on three human tumor cell strains. The alkaloid in the present invention comes from a natural medicine and food dual-purpose plant, has a novel structure, has a potential anti-tumor activity, can be used as an anti-tumor lead compound, and has good application prospects.

Description

Technical field [0001] The invention belongs to the technical field of extraction and separation, structure identification and biological activity research of active ingredients of medicinal plants, and specifically relates to a novel maca alkaloid and its preparation method and application. Background technique [0002] Maca ( Lepidiummeyenii Walp) (Brassicaceae), known as "Peruvian ginseng" and "South American ginseng", is a 1 to 2 year-old herbaceous plant belonging to the cruciferous family Lepidium. It is native to the Andes of South America at an altitude of 3500~4500 m. It is a commonly used medicine locally The use of plants and edible materials has a long history and reputation; because maca is rich in nutrients, rich in protein, carbohydrates, fatty acids, fiber, vitamins and minerals, it is recommended as an edible and safe food by the UN World Food and Agriculture Organization. Maca was also included in the new food resource catalog in May 2011; modern pharmacological...

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Problems solved by technology

[0002] Maca ( Lepidium meyenii Walp) (Brassicaceae), known as "Peruvian ginseng" and "South American ginseng", is a 1-2-year-old herb of the genus Brassicaceae. It is native to the South American Andes at an altitude of 3500-4500 m. It is a commonly used local medicine. Using plants and edible materials has a long history and reputation; because Maca is rich in nutrients, rich in protein, carbohydrates, fatty acids, fibers, vitamins and minerals, it is recommended by the United Nations World Food and Agriculture Organization as an edible safe food. In May 2011, maca was also included in the new food resource catalog; modern pharmacological activity studies have shown that maca has a variety of activities beneficial to human health, including improving fertility, anti-oxidation, improving immunity, and improving memory. , memory repair, regulation of hormone release, anti-tumor, blood lipid-lowering, etc. Further studies have shown that some of these biological activities are directly related to their fat-soluble chemical components, such as macaene and macamide, imidazole alkaloids, glucosinolates , sterols and steroids; introduced in China in early 1990, of which the Lijiang Shangri-La region in Yunnan is the main planting area, and the planting scale continues to expand. However, the chemical composition of the introduced Lijiang Maca lacks detailed chemical composition and biological activity research. This patent is Related research on it

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