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Preparation method of nano-microcapsule adopting chitosan-TPP-KGM as compound wall material

A technology of -TPP-KGM and nano-microcapsules, which is applied in the field of preparation of nano-microcapsules, can solve the problems of no high-strength spherical shape, complicated preparation process, loss of core material, etc., to achieve large-scale application and process The effect of mild and simple conditions and high load rate

Inactive Publication Date: 2018-05-01
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most studies use chitosan and TPP as wall materials to prepare microcapsules, but they often do not have high strength and good spherical shape, and are easy to form wrinkles and cracks, resulting in the loss of core materials.
In addition, biodegradable poly(lactic-co-glycolic acid) (PLGA) nanocapsules are also a commonly used carrier for bioactive substances. [1] , but its preparation process is complicated and the embedding rate is low, and organic solvents are needed in the use process, which is highly toxic and brings many problems
However, there is no research report on adding KGM to composite wall materials.

Method used

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  • Preparation method of nano-microcapsule adopting chitosan-TPP-KGM as compound wall material
  • Preparation method of nano-microcapsule adopting chitosan-TPP-KGM as compound wall material
  • Preparation method of nano-microcapsule adopting chitosan-TPP-KGM as compound wall material

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preparation example Construction

[0030] Preparation of Chitosan-TPP-KGM Nanocapsules

[0031] 1) Prepare a chitosan solution with a mass fraction of 0.2-0.5% (%, w / v), mix the TPP solution with the KGM solution, and add the mixture of the two substances into the chitosan solution dropwise.

[0032] 2) Add dropwise with magnetic stirring, until the dropwise addition of the mixture is complete, continue to stir at 10-50°C for 15-60min, collect the nanocapsules by centrifugation, and rinse with double distilled water three times to obtain chitosan-TPP-KGM composite nanocapsules.

[0033] Wherein, the mass ratio of chitosan / TPP / KGM is 6 / 1 / 1.2-6 / 1 / 3 respectively.

[0034] Chitosan-TPP-KGM nanocapsules coated casein phosphopeptides (CPPs)-Ca 2+ Application of Composite Core Material

[0035] CPP-Ca 2+ The complex was dissolved in Tris-HCl buffer (pH 7.4, 20 mM) to obtain a core material solution. Prepare a chitosan solution with a mass fraction of 0.2-0.5% (%, w / v), mix the TPP solution with the KGM solution, ...

Embodiment 1

[0039] Take chitosan in weighing paper and dissolve it in 1% acetic acid solution at room temperature, and obtain a chitosan solution with a mass fraction of 0.2% under magnetic stirring; weigh TPP powder and dissolve it in water to obtain a concentration of 3.3mg / ml of TPP solution; weigh KGM in a beaker, slowly add double distilled water with vigorous stirring to obtain a KGM solution with a concentration of 1 mg / ml. Weigh 0.5g CPP-Ca 2+ The complex is dissolved in a Tris-HCl buffer solution with a pH of 7.4 and 20 mmol / L to obtain a core material solution with a concentration of 50 mg / ml. Mix 0.6ml of TPP solution with 2.4ml of KGM solution, then add 1ml of core material solution, add the mixture of these three substances into 6ml of chitosan solution drop by drop at room temperature 20°C, stir while adding, drop all Stir and cross-link for 30 minutes after the addition is complete, at this time the mass ratio of chitosan / TPP / KGM is 6 / 1 / 1.2. After cross-linking, centrifu...

Embodiment 2

[0041] Take chitosan in weighing paper and dissolve it in 1% acetic acid solution at room temperature, and obtain a chitosan solution with a mass fraction of 0.3% under magnetic stirring; weigh TPP powder and dissolve it in water to obtain a concentration of 3.3mg / ml of TPP solution; weigh KGM in a beaker, slowly add double distilled water with vigorous stirring to obtain a KGM solution with a concentration of 1 mg / ml. Weigh 0.5g CPP-Ca 2+ The complex is dissolved in a Tris-HCl buffer solution with a pH of 7.4 and 20 mmol / L to obtain a core material solution with a concentration of 50 mg / ml. Mix 0.6ml of TPP solution with 2.4ml of KGM solution, then add 1ml of core material solution, add the mixture of these three substances into 6ml of chitosan solution drop by drop at room temperature 20°C, stir while adding, drop all Stir and cross-link for 30 minutes after the addition is complete, at this time the mass ratio of chitosan / TPP / KGM is 6 / 1 / 1.2. After cross-linking, centrifu...

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Abstract

The invention relates to a preparation method of a nano-microcapsule adopting chitosan-TPP-KGM as a compound wall material. The preparation method comprises the following steps: preparing chitosan solution with the mass percentage being 0.2-0.5 % (%, w / v), mixing TPP solution with KGM solution, and dropwise adding the mixed liquid of the two substances into the chitosan solution; carrying out magnetic stirring while dropwise adding is carried out till the mixed liquid is completely dropwise added, carrying out further stirring for 15-60 min at 10-50 DEG C, carrying out centrifugalizing, collecting nano-capsules, and carrying out flushing by adopting double distilled water, thus obtaining the chitosan-TPP-KGM compound nano-microcapsule, wherein the CPP-Ca<2+> compound is adopted as a modelmedicine for embedding. The loading rate of the microcapsule for the core material is high, the targeting and slow release effects are very good, and the microcapsule can be widely applied in the aspects including medicine slow release, food industry, biological functional materials, and the like.

Description

technical field [0001] The invention relates to a preparation method of nano-microcapsules using chitosan-TPP-KGM as a composite wall material, belonging to the field of biological materials. Background technique [0002] Nano-microcapsule technology refers to the use of nano-composite, nano-emulsification and nano-structure technologies to coat the materials in the capsule to form microcapsules in the nanoscale range (1-1000nm). In recent years, nano-microcapsule technology has become a hot spot in the field of food research and application. In the food industry, the substances used as core materials are mainly functional factors with biological activity. Nano-microcapsules can not only protect these active ingredients from volatilization, loss or oxidation, but also promote the slow release and absorption of active ingredients to Improve and enhance the flavor and nutrition of food. Wall materials mainly choose natural polymer materials with good solubility, hygroscopici...

Claims

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Application Information

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IPC IPC(8): A61K9/52A61K9/51A61K38/01A61K47/02A61K47/36A23L29/30A23P10/30
Inventor 齐崴黄仁亮张茜青屈彦宁苏荣欣
Owner TIANJIN UNIV
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