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Low-temperature-resistance nitrobacteria and application thereof

A technology of nitrifying bacteria and low temperature resistance, applied in the direction of bacteria, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problem that denitrification bacteria are not suitable for low temperature water bodies

Active Publication Date: 2018-05-04
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that existing denitrification bacteria are not suitable for low-temperature water bodies, and provide a strain of low-temperature-resistant nitrifying bacteria and its application

Method used

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  • Low-temperature-resistance nitrobacteria and application thereof
  • Low-temperature-resistance nitrobacteria and application thereof
  • Low-temperature-resistance nitrobacteria and application thereof

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specific Embodiment approach 1

[0013] Embodiment 1: A low-temperature-resistant nitrifying bacterium in this embodiment is Pseudomonas mohnii.M-8, which is preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, and the preservation address is Beichen, Chaoyang District, Beijing No. 3, No. 1 Yard, West Road, the preservation date is November 20, 2017, and the preservation number is: CGMCCNo.14936.

[0014] In this embodiment, high-efficiency low-temperature-resistant nitrifying bacteria screened out from fillers in Chinese-style reactors that treat Songhua River water are identified as Gram-negative bacteria and facultative anaerobic bacteria with a growth temperature of 2-35°C. The optimum temperature is 25-30°C, the length of the bacteria is 1-1.5 μm, the width is 0.5-1 μm, it is short rod-shaped, without spores. The colony is milky white translucent regular circle with a radius of 1-2mm, moist and shiny.

[0015] The DNA of Pseudomonas mohnii.M-8 wa...

specific Embodiment approach 2

[0017] Embodiment 2: The application of a strain of low-temperature-resistant nitrifying bacteria in this embodiment refers to the application of low-temperature-resistant nitrifying bacteria in the treatment of low-temperature water bodies.

[0018] In this embodiment, Pseudomonas mohnii.M-8 can grow at 2-35° C., and realize nitrification reaction in an aerobic state. At 2°C, in an aerobic state, with an initial ammonia nitrogen concentration of 50 mg / L, the bacteria NH 4 + The removal rate of -N was 92%. During the reduction of ammonia nitrogen, there was no accumulation of nitrite nitrogen and nitrate nitrogen, indicating that the bacteria could directly convert ammonia nitrogen into nitrogen and other gases. Compared with other low-temperature resistant strains, this strain has the advantages of adapting to lower temperature and higher denitrification efficiency.

specific Embodiment approach 3

[0019] Embodiment 3: This embodiment differs from Embodiment 2 in that: the application of low-temperature-resistant nitrifying bacteria in denitrification of low-temperature water bodies. Others are the same as in the second embodiment.

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Abstract

The invention relates to low-temperature-resistance nitrobacteria and application thereof and aims at solving the problem that existing denitrifying bacteria are not suitable for low-temperature waterbodies. The low-temperature-resistance nitrobacteria disclosed by the invention is Pseudomonas mohnii.M-8 and is preserved in the China General Microbiological Culture Collection Center, the preservation address is No.3 No.1 courtyard Beichen west road Chaoyang District Beijing, the preservation data is 20th November 2017, and the preservation serial number is CGMCC No.14936. The application disclosed by the invention is application of the low-temperature-resistance nitrobacteria in low-temperature water body treatment. Under the situations of 2 DEG C, an aerobic state and an initial ammonianitrogen concentration as 50mg / L, NH4<+>-N removing rate of the low-temperature-resistance nitrobacteria disclosed by the invention is 92%. The low-temperature-resistance nitrobacteria disclosed by the invention can be applied to the technical field of environmental microorganisms.

Description

technical field [0001] The invention relates to a low-temperature-resistant nitrifying bacterium and its application. Background technique [0002] Nitrogen is a common pollutant in natural water bodies. Excessive accumulation of nitrogen elements will cause eutrophication of the water environment and accelerated growth of algae, causing red tides and deterioration of water quality, resulting in the death of a large number of aquatic organisms. The main sources of nitrogen pollution in water are domestic sewage, industrial sewage and agricultural wastewater. The traditional biological nitrogen removal process mainly uses autotrophic bacteria to remove ammonia nitrogen in sewage by autotrophic nitrification. However, traditional autotrophic bacteria have strict temperature requirements. When the temperature drops below 10°C, the growth and metabolism of autotrophic bacteria will be greatly inhibited. [0003] In recent years, heterotrophic nitrifying bacteria have attracted...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/02C12R1/38
CPCC02F3/02C02F3/34C12N1/205C12R2001/38Y02W10/10
Inventor 马军吕东伟杨墨秦必达刘慧玲
Owner HARBIN INST OF TECH
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