CYP2D6 gene detection kit and CYP2D6 gene detection method
A gene detection and kit technology, applied in the fields of genetic engineering and molecular biology, can solve the problems of low sensitivity, high false positive rate and small throughput of CYP2D6 gene detection, achieve short detection cycle, reduce false positive rate, pass through high volume effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
no. 1 example
[0025] The present invention proposes a first embodiment, a CYP2D6 gene detection kit, comprising a primer set for specific amplification of a nucleic acid fragment containing the rs1801253 site, said primer set including upstream primers SEQ ID NO: 1, SEQ ID NO: 1, SEQ ID NO: ID NO:2, SEQ ID NO:3 or SEQ ID NO:4, and downstream primers SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7 or SEQ ID NO:8.
[0026] The CYP2D6 gene detection kit provided by this program includes a specific amplification primer set, which can specifically amplify nucleic acid fragments containing the rs1801253 site, reducing the possibility of amplifying non-target fragments, thereby The detection sensitivity of the rs1801253 site is high, and the false positive rate is low.
[0027] Preferably, the kit further includes a linker, the linker includes at least one of linker A, linker B, linker C, and linker D; the linker A consists of SEQ ID NO: 9 and SEQ ID NO: 10; The joint B consists of SEQ ID NO:11 and SEQ ID ...
no. 2 example
[0032] The present invention proposes a second embodiment, a gene detection method, comprising the following steps:
[0033] A. Utilize the amplification primer set to amplify the sample containing the rs1801253 site to obtain an amplified product; it is characterized in that the primer set includes upstream primers SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO : 3 or SEQ ID NO: 4, and downstream primers SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7 or SEQ ID NO: 8;
[0034] B. Connecting adapters to the amplification products to obtain library molecules;
[0035] C. Sequencing the library molecules by using the second-generation high-throughput gene sequencing technology to determine the genotype of the rs1801253 site.
[0036]The gene detection method provided by the invention uses a specific amplification primer set to amplify the nucleic acid fragment containing the rs1801253 site, thereby improving the detection sensitivity of the rs1801253 site and reducing the false positive rate of ...
no. 3 example
[0050] The present invention provides a third embodiment, a CYP2D6 gene detection method, using human whole blood genome sample 1 and human whole blood genome sample 2 as templates, respectively establishing a reaction system according to the following steps:
[0051] A1. For the above two samples, prepare a reaction system for amplifying the nucleic acid fragment of the No. 10 allele region of the CYP2D6 gene; prepare each reaction system according to the following ratio: 1.0 μL of human whole blood DNA molecules at 50 ng / μL; 2 ×long TaqMix (manufactured by Shenzhen Huayinkang Gene Technology Co., Ltd.) 10 μL; 10 μM first upstream primer 0.4 μL; 10 μM first downstream primer 0.4 μL; 8.2 μL deionized water; mix well and centrifuge. Then place the centrifuge tube in the PCR machine and set the reaction program: 5 minutes at 94°C; 20 seconds at 94°C, 20 seconds at 57°C, 25 seconds at 72°C, a total of 30 cycles ; Continue at 72° C. for 5 minutes; after the PCR reaction is complet...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More