Preparation method of protein-modified phytosterol liposome powder

A phytosterol lipid and protein modification technology, which is applied in the field of preparation of protein-modified phytosterol liposome powder, can solve the problems of low solubility and reduced embedding rate, achieve good solubility, improve stability, and improve redispersibility Effect

Active Publication Date: 2018-05-15
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this technical solution is to directly embed phytosterols with water-soluble protein hydrophobic groups. During the drying process, the structure of nanoparticles will be destroyed due to freeze-thaw

Method used

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  • Preparation method of protein-modified phytosterol liposome powder
  • Preparation method of protein-modified phytosterol liposome powder
  • Preparation method of protein-modified phytosterol liposome powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Lecithin and phytosterol are added to absolute ethanol at a ratio of 4:1 (w / w), so that the concentration of phytosterol is 1% (w / v), and stirred at room temperature to dissolve;

[0025] (2) Add soybean protein isolate to deionized water at a ratio of 1% (w / v), stir and dissolve at room temperature, adjust pH to 7.0, and hydrate overnight at 4°C;

[0026] (3) Slowly inject the phytosterol-lecithin ethanol solution obtained in step (1) into the protein solution obtained in step (2) according to the volume ratio of 1:4, and disperse at a high speed of 10000r / min for 5min; then under 80MPa High-pressure homogenization 8 times to obtain protein-modified phytosterol liposomes;

[0027] (4) The phytosterol liposomes obtained in the step (3) were subjected to rotary evaporation at 40°C under a vacuum of 0.11 MPa to remove ethanol, and freeze-dried to obtain a freeze-dried powder.

Embodiment 2

[0029] (1) Lecithin and phytosterol are added to absolute ethanol at a ratio of 3:1 (w / w), so that the concentration of phytosterol is 2% (w / v), and stirred at room temperature to dissolve;

[0030] (2) Add soybean protein isolate to deionized water at a ratio of 1% (w / v), stir and dissolve at room temperature, adjust pH to 7.0, and hydrate overnight at 4°C;

[0031] (3) Slowly inject the phytosterol-lecithin ethanol solution obtained in step (1) into the protein solution obtained in step (2) according to the volume ratio of 1:4, and disperse at a high speed of 10000r / min for 3min; then under 90MPa High-pressure homogenization 4 times to obtain protein-modified phytosterol liposomes;

[0032] (4) The phytosterol liposomes obtained in the step (3) were subjected to rotary evaporation at 40°C under a vacuum of 0.13 MPa to remove ethanol, and freeze-dried to obtain a freeze-dried powder.

Embodiment 3

[0034] (1) Lecithin and phytosterol are added to absolute ethanol at a ratio of 4:1 (w / w), so that the concentration of phytosterol is 4% (w / v), and stirred at room temperature to dissolve;

[0035] (2) Add soybean protein isolate to deionized water at a ratio of 0.5% (w / v), stir and dissolve at room temperature, adjust pH to 7.0, and hydrate overnight at 4°C;

[0036] (3) Slowly inject the phytosterol-lecithin ethanol solution obtained in step (1) into the protein solution obtained in step (2) according to the volume ratio of 1:4, and disperse at a high speed of 8000r / min for 5min; then under 80MPa High-pressure homogenization 4 times to obtain protein-modified phytosterol liposomes;

[0037] (4) The phytosterol liposomes obtained in the step (3) were subjected to rotary evaporation at 45° C. to remove ethanol under a vacuum of 0.11 MPa, and freeze-dried to obtain freeze-dried powder.

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Abstract

The invention discloses a preparation method of protein-modified phytosterol liposome powder and solves the problems that the water-solubility of phytosterol is poor and the blood fat reducing efficacy of the phytosterol is to be further improved. The method comprises the following steps: adding phytosterol and lecithin to absolute ethanol, performing stirring and dissolving at room temperature toobtain an organic phase; dissolving soybean protein isolate in water to obtain a water phase, slowly injecting the organic phase into the water phase under the condition of high-speed dispersion andperforming high-speed dispersion and uniform mixing, performing high-pressure homogenization and rotary evaporation to remove ethanol and then performing drying to obtain the protein-modified phytosterol liposome powder. The preparation method has simple process and less time consumption, the selected raw materials are safe and environmentally-friendly, the obtained liposome has good stability andhigh embedding rate and obviously improves the water-solubility of the phytosterol, and the liposome has good redissolution property and high solubility after being dried, can be stored for a long term and can be extensively applied to food and medicines for lowering the occurrence of hyperlipemia.

Description

technical field [0001] The invention relates to the field of food additives, in particular to a method for preparing protein-modified phytosterol liposome powder. Background technique [0002] Phytosterols are functional lipids with a cholesterol-like structure existing in plants, and are a general term for sterol compounds in plants. Phytosterols are white crystalline powder or granules at room temperature, odorless and tasteless, with a melting point of 130-170°C, insoluble in water, strong acids and strong alkalis, but soluble in various organic solvents, such as acetone, absolute ethanol, ether, Benzene, chloroform, petroleum ether, etc. [0003] In 1953, Pollak discovered that hypercholesterolemia could be treated by taking phytosterols, and since then started the research on the application of phytosterols in lowering cholesterol. Lees et al. found that a daily intake of 3 g of phytosterols can achieve the best cholesterol-lowering effect, which can reduce cholestero...

Claims

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Application Information

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IPC IPC(8): A23L33/105A23L33/115A23L33/185A23P10/30A23P10/35
CPCA23V2002/00A61K9/1271A61K31/575A61K31/685A61K45/06A61K47/42A23L33/105A23L33/115A23L33/185A23P10/30A23P10/35A61K2300/00A23V2200/224A23V2200/222A23V2200/3262A23V2200/216A23V2250/1842A23V2250/2136A23V2250/5488
Inventor 王春维祝爱侠程铭焦文佳夏廉臣王晓晖陈浩王铕
Owner WUHAN POLYTECHNIC UNIVERSITY
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