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Formula with function of simultaneously degrading aflatoxin and zearalenone

A technology of zearalenone and aflatoxin, which is applied in the field of microorganisms, can solve the problems of potential safety hazards and the joint toxicity of multiple mycotoxins.

Active Publication Date: 2018-05-15
河南德邻生物制品有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] However, at present, there are not many research reports on the combined toxic effects of multiple mycotoxins at home and abroad. Due to the lack of basic research in this area, scientists at home and abroad often only consider a single limit requirement for mycotoxins in the process of formulating feed hygiene standards. or a class of mycotoxins, but the joint toxicity caused by the mixed pollution of multiple mycotoxins cannot be considered, so there is a great potential safety hazard

Method used

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  • Formula with function of simultaneously degrading aflatoxin and zearalenone
  • Formula with function of simultaneously degrading aflatoxin and zearalenone
  • Formula with function of simultaneously degrading aflatoxin and zearalenone

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preparation example Construction

[0051] Further, the Lactobacillus acidophilus is inoculated and cultivated in the MRS liquid medium; the preparation method of the MRS liquid medium comprises:

[0052] (1) Dissolve tryptone, beef peptone, yeast extract, glucose, K 2 HPO 4 , sodium acetate, ammonium citrate, MgSO 4 , MnSO 4 ;

[0053] (2) Join Tween 80;

[0054] (3) Adjust pH, constant volume, sterilize, let stand for cultivation, and set aside.

[0055] Further, the Saccharomyces cerevisiae is inoculated and cultivated in YPD liquid medium; the preparation method of the YPD medium comprises:

[0056] (1) Dissolve yeast extract, tryptone, glucose with distilled water;

[0057] (2) Constant volume, sterilize, shake culture, set aside.

[0058] Further, the bacillus licheniformis is inoculated and cultivated in LB liquid medium; the preparation method of the LB medium comprises:

[0059] (1) Dissolve tryptone, yeast extract, and NaCl with distilled water;

[0060] (2) Adjust pH, constant volume, sterili...

Embodiment 1

[0123] Lactobacillus acidophilus was inoculated in MRS liquid medium;

[0124] The composition of MRS medium: tryptone 10g, beef peptone 10g, yeast extract 5g, glucose 20g, K 2 HPO 4 2g, sodium acetate 5g, ammonium citrate 2g, MgSO 4 0.2g, MnSO 4 0.05g, dissolved in 800mL distilled water, then added 1mL Tween 80, adjusted the pH to 6.2~6.6, then set the volume to 1000mL, at 121℃, 1.034×10 5 Sterilize under high-pressure steam for 20 minutes under Pa condition; after static cultivation at 37°C for 24 hours, it is ready for use.

[0125] Saccharomyces cerevisiae was inoculated in YPD liquid medium;

[0126] Composition of YPD medium: 10g of yeast extract, 20g of tryptone, 20g of glucose, then set the volume to 1000mL, at 121℃, 1.034×10 5 Sterilize under high-pressure steam for 20 minutes under Pa condition; shake culture under the condition of 30° C. and 180 rpm for 24 hours, and then set aside.

[0127] Bacillus licheniformis was inoculated in LB liquid medium;

[012...

Embodiment 2

[0134] Lactobacillus acidophilus was inoculated in MRS liquid medium;

[0135] The composition of MRS medium: tryptone 8g, beef peptone 5g, yeast extract 15g, glucose 23g, K 2 HPO 4 1g, sodium acetate 4g, ammonium citrate 1g, MgSO 4 0.1g, MnSO 4 0.03g, dissolved in 900mL distilled water, then added 2mL Tween80, adjusted the pH to 6.2-6.6, then set the volume to 1000mL, at 130℃, 1.034×10 5 Sterilize under high-pressure steam for 30 minutes under Pa condition; after static cultivation at 37°C for 18 hours, it is ready for use.

[0136] Saccharomyces cerevisiae was inoculated in YPD liquid medium;

[0137] Composition of YPD medium: 8g of yeast extract, 25g of tryptone, 15g of glucose, then set the volume to 1000mL, at 115℃, 1.034×10 5 Sterilize under high-pressure steam for 20 minutes under Pa condition; shake culture under the condition of 30° C. and 180 rpm for 24 hours, and then set aside.

[0138] Bacillus licheniformis was inoculated in LB liquid medium;

[0139] C...

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Abstract

The invention provides a formula with a function of simultaneously degrading aflatoxin and zearalenone. The formula is an effective and safe method for biodegrading mycotoxins. The formula has the advantages that composition conditions for bacillus licheniformis, saccharomyces cerevisiae and lactobacillus acidophilus are optimized by the aid of response surface regression designs, and the optimalAFB1 (aflatoxin 1) and ZEA (zearalenone) degradation rates can reach 45.49% and 44.90% when the volume ratio reaches 1:1:1; then the bacillus licheniformis, the saccharomyces cerevisiae and the lactobacillus acidophilus are compatible with mycotoxins degradation enzymes generated by aspergillus niger by means of fermentation, and the AFB1 and ZEA degradation rates can reach 63.95% and 73.51% whenthe volume ratio reaches 3:2 and are increased by 40.58% and 63.72% as compared with previous single composite probiotics degradation rates; as shown by test results, the formula is the effective method for simultaneously degrading the AFB1 and the ZEA which are two types of mycotoxins by the aid of the mycotoxins degradation enzymes generated by composite probiotics and the aspergillus niger by means of fermentation.

Description

technical field [0001] The invention relates to the technical field of microorganisms, and more specifically relates to a formula capable of simultaneously degrading aflatoxin and zearalenone. Background technique [0002] According to the estimates of the United Nations Food and Agriculture Organization (FAO), about 25% of the food and feed in the world are contaminated with mycotoxins every year, and an average of 2% of the food is inedible. About a third of feed and feed samples in the Asia-Pacific region tested positive for mycotoxins. [0003] These food and feed ingredients are mainly contaminated by aflatoxin B1 (AFB 1 ), zearalenone (ZEA), deoxynivalenol (DON) and fumonisin B1 (FB1) contamination. In 2003, the survey results of mycotoxins in feed materials and compound feeds in China showed that 88%, 84%, 77% and 60% of corn contained T-2 toxin, aflatoxin, fumonisin and ochratoxin a. And all corn contains vomitoxin and zearalenone. More than 90% of the compound ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18C12N9/00A23L5/20C12R1/10C12R1/865C12R1/23
CPCA23L5/25A23L5/28C12N1/18C12N1/20C12N9/00
Inventor 尹清强黄玮玮常娟王平李庆华党晓伟宋安东王国强刘超齐朱群高天增陈志杰卢富山
Owner 河南德邻生物制品有限公司
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