Preparation method of phellinus igniarius nutritive meal replacement powder
A technology of meal replacement powder and Phellinus, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, and the functions of food ingredients, can solve the problems of difficulty in meeting market demand, difficult fruiting bodies of Phellinus, and high production costs, and achieve The effect of enhancing human immunity, shortening the cultivation time, and accelerating the germination speed
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Embodiment 1
[0019] ① Put Phellinus japonica stored in a refrigerator at 1°C for activation at room temperature 24 hours in advance, then transfer to a new Phellinus japonica culture medium, and cultivate at 20°C until the tube is full of Phellinus mycelium.
[0020] ②Make shake flask liquid medium, the medium formula is: bran 5%, potato 20%, magnesium sulfate 0.1%, potassium dihydrogen phosphate 0.2%, peptone 0.2%, glucose 1%, brown sugar 1.5%, VB1 10mg / L , defoaming agent 1 drop / bottle, the activated Phellinus japonica seed was transferred to the shake flask liquid medium, placed on a shaker for shaking culture, the shaker speed was 200rpm, and cultivated for 140h at a temperature of 23°C.
[0021] ③To make the fermentation medium, select fresh and mildew-free corn, wolfberry, licorice, cistanche, Cynomorium, soak in water for 12 hours, then heat and cook the corn until there is no white heart, remove, drain the surface water, licorice, Cistanche and Cynomorium cut into 5mm slices, and f...
Embodiment 2
[0026] ① Put Phellinus japonica stored in a refrigerator at 3°C for activation at room temperature 24 hours in advance, then transfer to a new Phellinus japonica medium, and culture at 23°C until the tube is full of Phellinus mycelium.
[0027] ②Make shake flask liquid medium, the medium formula is: bran 7%, potato 21%, magnesium sulfate 0.2%, potassium dihydrogen phosphate 0.3%, peptone 0.3%, glucose 2%, brown sugar 2.5%, VB1 10mg / L , antifoaming agent 1 drop / bottle, the activated Phellinus japonica seed was transferred to the shake flask liquid medium, placed on a shaker for shaking culture, the shaker speed was 210rpm, and cultivated for 150h at a temperature of 24°C.
[0028] ③To make fermentation medium, select fresh and mildew-free corn, wolfberry, licorice, cistanche, Cynomorium, soak in water for 14 hours, then heat and cook the corn until there is no white heart, remove, drain the surface water, licorice, Cistanche and Cynomorium cut into 5mm slices, and finally mix...
specific Embodiment 3
[0032]①Activation of the Phellinus mother species, the Phellinus mother species stored in the refrigerator at 5°C was activated at room temperature 24 hours in advance, and then transferred to the new Phellinus mother seed medium, and cultivated at 25°C until the Phellinus fungus Full tube of silk hair for spare;
[0033] ② Make shake flask liquid culture medium, wherein the medium formula is: bran 8%, potato 22%, magnesium sulfate 0.3%, potassium dihydrogen phosphate 0.4%, peptone 0.4%, glucose 3%, brown sugar 3.5%, VB 110mg / L, 1 drop / bottle of antifoaming agent, transfer the activated Phellinus japonica parent species into the shake flask liquid culture medium, place it on a shaker for shaking culture, the shaker speed is 220rpm, and cultivate at 25°C for 160h;
[0034] ③To make the fermentation medium, select fresh and mildew-free corn, wolfberry, licorice, cistanche, and Cynomorium, soak them in water for 16 hours, then heat and cook the corn until there is no white heart...
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