Biological dressing as well as preparation method and application thereof

A biological dressing and aqueous solution technology, applied in medical science, absorbent pads, bandages, etc., can solve the problems of no research on the biocompatibility of the composite film, easy dissolution and fragility of the silk film, and no practicality, etc., and achieve success Transplantation, achieving cell suspension retention, and beneficial cleaning effects

Inactive Publication Date: 2018-05-18
罗卫
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Silk fibroin is a fibrous protein obtained by degumming silkworm cocoons. It has good tissue compatibility and has an excellent promotion effect on cell growth. It is an ideal raw material for the manufacture of biomedical materials. Soluble and fragile, it is not practical. Therefore, the current research on silk fibroin film is only to cooperate with other components to improve its breaking strength, elongation, water permeability and other aspects. For the biophase of the obtained composite film Compatibility is not studied, especially for the compatibility of composite membranes and melanocytes

Method used

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  • Biological dressing as well as preparation method and application thereof
  • Biological dressing as well as preparation method and application thereof
  • Biological dressing as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0024] The preparation method of the biological dressing provided in this embodiment comprises the following steps:

[0025] (1) The silk is placed in a Na mass concentration of 0.2% 2 CO 3 Remove sericin in aqueous solution, wash and dry to obtain silk fibroin powder;

[0026] (2) adding CaCl to the silk fibroin powder obtained in step (1) 2 : CH 2 CH 2 OH: H 2 Dialysis was carried out in a ternary solvent with a molar ratio of O of 1:2:8 for 3 days, followed by concentration and centrifugation to obtain a silk fibroin aqueous solution with a concentration of 2wt%;

[0027] (3) Take 100 mL of the silk fibroin aqueous solution prepared in step (2), add 30 mL of a 2% carboxymethyl cellulose sodium aqueous solution and 10 mL of a 10% 10% gelatin aqueous solution, and perform mechanical stirring for 2 h , and then added to a foamer for foaming for 20 minutes, poured into a flat-bottomed mold to be flattened, and finally freeze-dried in a freeze dryer to form a three-dimensi...

Embodiment 2

[0030] The preparation method of the biological dressing provided in this embodiment comprises the following steps:

[0031] (1) The silk is placed in a Na mass concentration of 0.1% 2 CO 3 Remove sericin in aqueous solution, wash and dry to obtain silk fibroin powder;

[0032] (2) adding CaCl to the silk fibroin powder obtained in step (1) 2 : CH 2 CH 2 OH: H 2 Dialysis was performed in a ternary solvent with a molar ratio of O of 1:2:8 for 2.5 days, followed by concentration and centrifugation to obtain a silk fibroin aqueous solution with a concentration of 2.2wt%;

[0033] (3) Take 100 mL of the silk fibroin aqueous solution prepared in step (2), add 30 mL of a 1.8% sodium carboxymethyl cellulose aqueous solution and 10 mL of a 11% 11% aqueous gelatin solution, and perform mechanical stirring for 1.5 h, and then added to a foamer for foaming for 25 minutes, poured into a flat-bottomed mold to be flattened, and finally freeze-dried in a freeze dryer to form a three-di...

Embodiment 3

[0036] The preparation method of the biological dressing provided in this embodiment comprises the following steps:

[0037] (1) The silk is placed in a Na mass concentration of 0.3% 2 CO 3 Remove sericin in aqueous solution, wash and dry to obtain silk fibroin powder;

[0038] (2) adding CaCl to the silk fibroin powder obtained in step (1) 2 : CH 2 CH 2OH: H 2 The molar ratio of O was 1:2:8 ternary solvent for dialysis for 4 days, and then concentrated and centrifuged to obtain a silk fibroin aqueous solution with a concentration of 1.8wt%;

[0039] (3) Take 100 mL of the silk fibroin aqueous solution prepared in step (2), add 30 mL of a 2.2% mass concentration of sodium carboxymethyl cellulose aqueous solution and 10 mL of a 9% mass concentration of gelatin aqueous solution, and perform mechanical stirring for 2.5 h, and then added to a foamer for foaming for 15 minutes, poured into a flat-bottomed mold and flattened, and finally freeze-dried in a freeze dryer to form ...

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Abstract

The invention provides a biological dressing as well as a preparation method and an application thereof. The biological dressing is a blend membrane formed by silk fibroin, sodium carboxymethyl cellulose and gelatin, the biological dressing has biodegradability and can promote tissue repair. Besides, the biological dressing is combined with melanocytes and keratinocytes in an autologous epidermiccell suspension, is endowed with good bioactivity and has better biocompatibility with leucoderma skin damage wounds in transplantation therapy of leucoderma, melanocytes and keratinocytes in the autologous epidermic cell suspension have higher cell survival rates on the biological dressing, thus, the autologous epidermic cell suspension can be transplanted onto the biological dressing and cover the leucoderma skin damage wounds, the cell suspension can be retained instead of flowing, and finally, successful transplantation of autologous epidermic cells of the leucoderma skin damage wounds isrealized.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a biological dressing and a preparation method and application thereof. Background technique [0002] Vitiligo is a kind of skin depigmentation disease with an incidence rate of 1% to 2%. It occurs mostly in young people, and it is more common in exposed parts such as face, neck and limbs, which often causes huge mental stress and physical and mental trauma to patients. With the development of the economy, people's requirements for beauty are getting higher and higher, and skin color as the "facade" of beauty has attracted more and more attention. [0003] The basic pathological changes of vitiligo are the reduction or disappearance of the number of melanocytes in the skin lesions. At present, the traditional methods of treating vitiligo are mainly autologous epidermal transplantation, autologous melanocyte transplantation and autologous epidermal cell suspension t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/32A61L15/28A61L15/40A61L15/64A61L15/44A61L15/42
CPCA61L15/28A61L15/32A61L15/325A61L15/40A61L15/425A61L15/44A61L15/64A61L2300/412C08L89/00C08L1/286
Inventor 罗卫陈杰
Owner 罗卫
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