Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of cell penetrating peptide and cell penetrating peptide complex and the application of both

A technology for penetrating peptides and complexes. It is applied to non-active ingredients in medical preparations, peptides, depsipeptides, etc. to reduce complications, increase drug utilization efficiency, and achieve high safety.

Active Publication Date: 2019-11-19
HOSPITAL OF STOMATOLOGY SUN YAT SEN UNIV +1
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The study of Polybia-MPI anti-tumor cationic polypeptides shows that such a structure is conducive to the electrostatic combination of cationic CPPs and tumor cell membranes, which not only provides a molecular basis for the development of penetrating peptide drug carriers targeting tumor cells, but also provides a basis for the development of penetrating peptides that destroy tumor cell membranes. The penetrating peptide drug platform provides the possibility, but so far there are few reports of this promising compound drug that destroys tumor cell membranes

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of cell penetrating peptide and cell penetrating peptide complex and the application of both
  • A kind of cell penetrating peptide and cell penetrating peptide complex and the application of both
  • A kind of cell penetrating peptide and cell penetrating peptide complex and the application of both

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0108] Example 1. Screening, sequence design and synthesis of cell-penetrating peptides

[0109] 1. Screening of tumor cell-penetrating peptides by phage display technology

[0110] Using the tumor cell membrane protein as the target molecule, the "T7Select Human Lung Cancer cDNA Library (Novagen)" was used to enrich the phage bound to the cell membrane protein, and repeated cycles of phage binding, elution and enrichment until the recovery rate of the phage library no longer increased After randomly selecting 48 monoclonal phages and amplifying them, the binding efficiency of each monoclonal phage to the tumor cell membrane protein was detected by a spectrophotometer. Monoclonal phages with a high binding rate to tumor cell membrane molecules were selected. After PCR and sequence analysis, it was determined that the amino acid sequence 1 might be a cell-penetrating peptide, with a total of 54 amino acids, including 29 basic amino acids, accounting for 53.7%. Lysine Acid: arg...

Embodiment 2

[0111] Example 2. Design and preparation of penetrating peptide markers, in vivo and in vitro cell labeling and permeability studies

[0112] 1. Synthesis of penetrating peptide marker FITC-TCPP

[0113] FITC-TCPP was synthesized according to the above amino acid sequence of TCPPs by solid-phase synthesis method (synthesized by Shanghai Qiangyao Biotechnology Co., Ltd., a commercial synthesis company). The direction of synthesis is from C to N-terminus. First, the carboxyl group of the carboxyl-terminal amino acid of the peptide chain to be synthesized is connected to an insoluble polymer resin in a covalent bond structure, and then the amino acid bound to the solid-phase carrier is used as the amino group. The components are deprotected by amino groups and reacted with excess activated carboxyl components to lengthen the peptide chain. Such steps are repeated many times, that is, condensation-washing-deprotection-neutralization and washing-the next round of condensation, and...

Embodiment 3

[0119] Example 3. Design and preparation of penetrating peptide cytotoxic drugs and their anti-tumor effects

[0120] 1. Design and preparation of tumor cell penetrating peptide doxorubicin (TCPP-DOX) cytotoxic drug

[0121] In the preparation process of the penetrating peptides TCPP and FITC-TCPP in the above-mentioned Example 2, doxorubicin (purchased from Italian Pharmacia) was coupled respectively to prepare FITC-TCPP-Dox (TCPP-Dox) and FITC-Dox. The polypeptide was purified by HPLC, and the purified solution was lyophilized and protected from light to obtain a pure product. Before being sealed and packaged and stored at -20 degrees, a small amount of the finished peptide was taken for molecular weight identification by MS and purity identification by HPLC analysis. The identification results showed that FITC-TCPP-Dox drug and FITC-Dox drug were successfully synthesized.

[0122] 2. Tumor cell killing experiment of tumor cell penetrating peptide doxorubicin FITC-TCPP-Dox ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Provided are a cell penetrating peptide and a cell penetrating peptide complex, as well as an application thereof. The cell penetrating peptide provided by the present invention is selected from the following amino acid sequences: an amino acid sequence as represented by sequence 1, sequence 2, sequence 3, sequence 4, or sequence 5, or an amino acid sequence having at least 50% homology with any one from among sequences 1-5.

Description

technical field [0001] The present invention relates to a cell membrane penetrating peptide and a cell penetrating peptide complex, and also relates to the application of the cell penetrating peptide and the cell penetrating peptide complex. Background technique [0002] The cell membrane is a phospholipid bilayer, which is a semipermeable barrier between the inner and outer environments of the cell. It has selective permeability and ensures the relative stability of the inner environment of the cell. The cell membrane barrier can prevent certain substances, especially harmful substances, from entering the cells from the blood, and helps to maintain the normal functional activities of the cells. Penicillin acts on the cell wall of prokaryotic cells. The structure of penicillin is similar to that of D-alanyl-D-alanine in the mucinous peptide structure of the cell wall. It can compete with the latter for transpeptidase, hinder the formation of mucinous peptide, and cause cell ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/00A61K47/64A61K51/08A61K51/06A61K49/00
CPCA61K49/0056A61K51/065A61K51/08C07K14/00C07K19/00C07K2319/10
Inventor 汪华李小龙赵龙张雁
Owner HOSPITAL OF STOMATOLOGY SUN YAT SEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com