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Molting hormone extracting method

A technology of ecdysone and extract, which is applied in the direction of steroids and organic chemistry, can solve the problems of affecting the biological activity of ecdysone, increasing the processing cost of enterprises, and the long production period of column chromatography, so as to achieve short cycle and short extraction route , the effect of fast time

Inactive Publication Date: 2018-05-29
YUNNAN HANDE BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] (1) At present, due to the strong water solubility of ecdysteroids, most extractions use water as the extraction solvent, and after extraction, they are concentrated, column chromatography, and recrystallized; during the process of concentrating the water extract, due to the boiling point of water, The concentration temperature is too high and the concentration time is long, which affects the biological activity of ecdysone;
[0011] (2) In most of the extraction methods reported in literature and patents, the obtained water extract must be separated and purified by macroporous resin. In industrial production, it must be concentrated again with alcohol water or acetone water. The time is long and the temperature is high, which once again affects the biological activity of ecdysone; due to the column chromatography process, the production cycle is lengthened and the cost is increased;
[0012] (3) Due to the use of a large amount of methanol or ethanol water or extraction methods in the traditional process, due to the water-soluble characteristics of ecdysone, the extraction yield is greatly reduced during production; and a large amount of waste water is generated during the production process, which is directly discharged Affect the environment and water bodies, and increase the production cost after wastewater treatment; the investment in the environment during industrial production increases the processing cost of the enterprise from another angle;
[0013] (4) The traditional extraction process needs to go through water extraction, concentration, and column chromatography production. Column chromatography has a long production period and a large cost investment

Method used

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  • Molting hormone extracting method

Examples

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Effect test

Embodiment 1

[0028] Embodiment 1: this extracts the method for ecdysone from dew grass, concrete operations are as follows:

[0029] (1) Add 100kg of dew grass powder with ecdysterone content of 3.1% (water content 8%), add 300L methanol to the crushed product and soak for 4 hours, filter, filter residue soaked in 300L methanol for 4 hours, filter, and combine the two filtrates , concentrated under reduced pressure at 65°C to 1 / 5 of the original volume to obtain a yellow-green ecdysone extract;

[0030] (2) Put the ecdysone extract in a 100L container, add activated carbon and diatomaceous earth at a ratio of 100 g of decolorizing flocculant per L of ecdysone extract, the mass ratio of activated carbon to diatomite is 2:1, Stir and decolorize at 40°C for 4 hours, filter under reduced pressure, remove activated carbon and diatomaceous earth, and obtain a light yellow transparent methanol liquid; evaporate the methanol liquid on a rotary evaporator at 65°C under reduced pressure to obtain ye...

Embodiment 2

[0033] Embodiment 2: this extracts the method for ecdysone from dew grass, concrete operation is as follows:

[0034](1) Take 100kg of dew grass powder with ecdysterone content of 3.1% (water content 5%), add 400L methanol to the crushed product and soak for 3 hours, filter, soak the filter residue in 300L methanol for 3 hours, filter, and filter the filter residue with methanol again Soak, combine the filtrates for 3 times, and concentrate under reduced pressure at 60°C to 1 / 5 of the original volume to obtain a yellow-green ecdysone extract;

[0035] (2) Put the ecdysone extract in a container, add activated carbon and diatomite at a ratio of 80 g of decolorizing flocculant per L of ecdysone extract, the mass ratio of activated carbon to diatomite is 3:1, at 45 Stir and decolorize at ℃ for 3 hours, filter under reduced pressure, filter out activated carbon and diatomaceous earth, and obtain a light yellow transparent methanol liquid; evaporate the methanol liquid on a rotary ...

Embodiment 3

[0038] Embodiment 3: this extracts the method for ecdysone from dew grass, concrete operations are as follows:

[0039] (1) Put 100kg of dew grass powder with 3.1% ecdysterone content (water content 7%), add 350L methanol-acetone mixture (methanol and acetone by volume ratio 1:0.5) to the crushed product, soak for 3 hours, filter, The filter residue was once again soaked in 300L methanol-acetone mixture for 4 hours, filtered, and the two filtrates were combined, concentrated under reduced pressure at 65°C to 1 / 5 of the original volume to obtain a yellow-green ecdysone extract;

[0040] (2) Put the ecdysone extract in a 100L container, add activated carbon and diatomite at the ratio of 120 g of decolorizing flocculant per L of ecdysone extract, the mass ratio of activated carbon to diatomite is 5:1, Stir and decolorize at 50°C for 4 hours, filter under reduced pressure, remove activated carbon and diatomaceous earth, and obtain a light yellow transparent methanol liquid; evapor...

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Abstract

The invention discloses a molting hormone extracting method. According to the method, methyl alcohol or a methyl alcohol-acetone mixed solution is used to soak cyanotis arachnoidea to extract moltinghormone, and then the molting hormone is obtained through decoloring, crystalizing and recrystallizing. The method is simple and easy to operate, does not need column chromatography, is low in cost and short in period, and is suitable for industrial application and market promotion.

Description

technical field [0001] The invention relates to an industrial production method for extracting ecdysone from dried dew grass. Background technique [0002] "Ecdysone", also known as "peeling hormone", is an active substance extracted from the root of the Commelinaceae plant Pearl Dew. According to different purity, it can be divided into white, off-white, light yellow or light brown crystalline powder; molecular formula C 27 h 44 o 7 ; [0003] [0004] Ecdysone acts on the human body to promote collagen synthesis, anti-arrhythmia, anti-fatigue; promote cell growth, stimulate dermal cell division, and eliminate cholesterol in the body; lower blood lipids, inhibit blood sugar rise; clear the meridians, activate collaterals, dehumidify and relieve pain and other physiological functions Active; it is a natural anti-cancer preparation; since the dew grass ecdysone came out in 1976, irregular tablets, injections, and ointments have been developed. It is used in the folk f...

Claims

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Application Information

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IPC IPC(8): C07J9/00
CPCC07J9/00
Inventor 马回民杨青春陈梦达李彦兰
Owner YUNNAN HANDE BIO TECH
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