Method for screening autotrophic nitrobacteria resisting low temperature and high salt and application of autotrophic nitrobacteria
A low-temperature resistant, nitrifying bacteria technology, applied in chemical instruments and methods, bacteria, water pollutants, etc., can solve problems such as inability to handle, attenuation, and activity attenuation of nitrifying bacteria, so as to improve adaptability, difficulty in preservation, and fermentation process Optimized effect
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Embodiment 1
[0036] 1) the acquisition of cultures,
[0037] Put 10mL of activated sludge from the aerobic pool of a sewage treatment system in a certain aquatic product processing plant in Dalian into 100mL of distilled water, add some glass beads 120R to break and wash for 30 minutes, and centrifuge to recover the sludge after cleaning, and wash it 3 times;
[0038] Put all the cleaned activated sludge into 100mL autotrophic nitrifying bacteria enrichment medium for enrichment culture, insert 1.6g / L sodium chloride into the medium, culture at 150R, 20°C for 7 days; The initial concentration was 122mg / L, and the concentration of ammonia nitrogen in the culture medium was 65mg / L at the end of the culture. After the cultivation, take 10mL of the culture solution and insert it into 100mL of new enrichment medium, insert 3.2g / L sodium chloride into the medium, culture at 150R, 20℃ for 14 days, the initial concentration of ammonia nitrogen in the medium is 126mg / L, and culture At the end of t...
Embodiment 2
[0049] Utilize the bacteria system described in above-mentioned embodiment 1 to the small test evaluation of ammonia nitrogen wastewater treatment:
[0050] A small-scale evaluation test was carried out with a certain aquatic product processing wastewater. The influent ammonia nitrogen of the aerobic biochemical system of the aquatic product processing plant was about 120mg / L, the effluent ammonia nitrogen was about 110mg / L, the system temperature was about 20°C, and the concentration of chloride ions in the water body was 2000mg About / L, the mud-water mixture 50L of this aerobic pond is packed in the 100L open container and adds respectively above-mentioned embodiment to obtain bacterial agent simultaneously, and with nitrifying bacteria NB1 and nitrifying bacteria NB2 as contrast, and utilize air pump to carry out aeration to will Dissolved oxygen control In the test, intermittent water changes were used, changing once every 24 hours. The test temperature was 20-25°C, and th...
Embodiment 3
[0055] Evaluation of Continuous Dosing Effect of Low Temperature and High Salt Nitrifying Bacteria
[0056]The sewage treatment system of a pesticide enterprise, the effective pool volume of the aerobic pool is 3000 liters, the sludge concentration of the aerobic pool is about 3000mg / L, the water temperature is about 18-20℃ in winter, the residence time is about 24h, and the chloride ion concentration of the influent is 2700mg / L. The concentration of ammonia nitrogen in the influent is 15-20mg / L, the concentration of ammonia nitrogen in the effluent is 15-20mg / L, and the nitrification capacity of the system is basically zero. Set up the nitrifying bacteria continuous culture device of 2 liters of scales in this enterprise sewage treatment station, obtain low temperature and high salt resistance nitrifying bacterium agent continuous culture and add by above-mentioned embodiment, add the nitrifying bacteria fermentation of 0.75 liters of every 24h to the biochemical pond. The fe...
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