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Fibrous scaffold, and preparation method and application thereof

A fiber scaffold and fiber technology, applied in the field of bioengineering, can solve the problems of small gaps, acid, alkali and high temperature resistance, etc., and achieve the effect of simple steps, high mechanical strength and large gaps

Active Publication Date: 2018-06-05
GUANGDONG UNISUN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Based on this, it is necessary to provide a fiber scaffold and its preparation method and application for fiber scaffolds that have problems such as small voids, acid and alkali resistance, high temperature resistance, and hydrophobicity.

Method used

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  • Fibrous scaffold, and preparation method and application thereof
  • Fibrous scaffold, and preparation method and application thereof
  • Fibrous scaffold, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0047] Such as figure 1Shown, the preparation method of fiber scaffold comprises:

[0048] S100: A fiber sheet made of polymer fibers interwoven to form a sheet-like structure or a cloth-like structure

[0049] S200: Washing and drying the fiber sheet.

[0050] Preferably, double-distilled water or even ultrapure water is used for cleaning, and ultrasonic cleaning can be used for cleaning, and the cleaning time is 15 to 35 minutes. The drying method can be carried out in an oven at 60-80°C or a similar environment.

[0051] Preferably, step S100 further includes the step of soaking the fiber sheet with 20-30% hydrogen peroxide. The concentration of the hydrogen peroxide solution is preferably 20% to 30%, and the soaking time can be 3 to 6 hours when the temperature is 65 to 80°C.

[0052] S300: Perform pre-modification activation treatment on the dried fiber sheet to obtain an activated fiber sheet.

[0053] Specifically, the fiber sheet is added to an activator with a co...

Embodiment 1

[0067] Fibrous scaffolds and their preparation

[0068] The polymer fibers made of polyester carbonate are interwoven to form a sheet-like fiber sheet, which undergoes the following series of treatments:

[0069] Cleaning pretreatment: add the fiber sheets to ultrapure water, use ultrasonic cleaning for 20 minutes, then blow dry with nitrogen, soak in 25% hydrogen peroxide solution at 65°C for 4 hours, and then use ultrapure water to ultrasonically Wash for 30 minutes and dry in a drying oven at 75°C for use.

[0070] Fiber sheet activation catalysis: the fiber sheet that pretreatment obtains is added to the bis-epoxidized butadiene that concentration is 5mmol / g, n-butyl glycidyl ether mixture (the mol ratio of bis-epoxidized butadiene and n-butyl glycidyl ether is 1:1), the temperature for activating the catalyst was 50°C, after 18 hours, it was taken out and cleaned ultrasonically with ultrapure water for 30 minutes, and then dried with nitrogen to obtain an activated fiber...

Embodiment 2

[0075] Fibrous scaffolds and their preparation

[0076] The polymer fibers made of polypropylene are interwoven to form a sheet-like fiber sheet, which undergoes the following series of treatments:

[0077] Cleaning pretreatment: Add the fiber sheets to ultrapure water, use ultrasonic cleaning for 15 minutes, then blow dry with nitrogen, soak in 30% hydrogen peroxide solution at 70°C for 3 hours, and then use ultrapure water to ultrasonically Wash for 30 minutes and dry in a drying oven at 75°C for use.

[0078] Activation and catalysis of fiber sheets: add the pretreated fiber sheets to bis-epoxidized butadiene with a concentration of 2mmol / g, and activate the catalyst at a temperature of 45°C. After 24 hours, take them out and ultrasonically clean them with ultrapure water for 30 minutes, and then use Blow dry with nitrogen to obtain an activated fiber sheet.

[0079] Attachment modification: Dissolve lysine in 0.5mol / L KOH solution, prepare it into a lysine alkali solutio...

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Abstract

The invention provides a fibrous scaffold, a preparation method for the fibrous scaffold and application of the fibrous scaffold to animal cell culture. The fibrous scaffold comprises polymeric fibersand a positive-charge modification layer covering the surfaces of the polymeric fibers, wherein the diameters of the polymeric fibers are in a range of 10 to 60 [mu]m, and surface positive charge concentration is 0.2 to 0.3 mmol / g. The fibrous scaffold provided by the invention has greater gaps, higher mechanical strength, stable performance and complete hydrophilicity, can be cyclically utilized, and is beneficial for culture and growth of cells. The preparation method for the fibrous scaffold is simple in steps and suitable for production of various scales.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a fiber support and its preparation method and application. Background technique [0002] At present, microcarriers are widely used in large-scale cell culture technology, such as cell culture, production of biological products such as antibodies, cytokines, and viruses. The reason why it is widely used is that it is a kind of non-toxic substance with good binding force to cells, and it has a large specific surface area and specific volume, and the cell culture area is large, and it saves space. [0003] The current microcarriers include silk fibroin and chitosan macroporous microcarriers, chitosan and gelatin mixed microcarriers, etc. However, spherical microcarriers have the problem that cells can only attach to the surface and have a small area-to-volume ratio, while porous microcarriers have gaps that facilitate cell attachment, but the gaps are easily blocked and affect oxygen...

Claims

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Application Information

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IPC IPC(8): C12N5/07D06M13/342D06M101/32D06M101/20D06M101/34
CPCC12N5/0068C12N2533/30D06M13/342D06M2101/20D06M2101/32D06M2101/34
Inventor 高毅翁骏彭公泽张志
Owner GUANGDONG UNISUN BIOTECHNOLOGY CO LTD
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