Hydrogel containing protease-inorganic hybrid nanoflower, and preparation method and applications thereof

Abstract

The invention discloses a hydrogel type enzyme catalytic material which contains protease-inorganic hybrid nanoflower and is prepared through secondary immobilization synthesis, and a preparation method and applications thereof. According to the preparation method, protease is taken as an organic component, and is subjected to self-assembling with inorganic metal ions so as to obtain the protease-inorganic hybrid nanoflower; the protease-inorganic hybrid nanoflower is embedded with a gel carrier system which is excellent in biocompatibility and contains at least two hydrophilic polymers, repeat freezing-unfreezing method is adopted to obtain the hydrogel containing protease-inorganic hybrid nanoflower. The unique tridimensional network of the hydrogel containing protease-inorganic hybrid nanoflower is capable of realizing stable existing of enzymes in a catalytic system, and secondary immobilization is achieved. According to the preparation method, secondary immobilization is adopted,so that protease is protected from external environment influences, enzyme stability is improved, enzyme reusability is improved. Separation of the catalytic material with reactants is not necessary,so that a step of separation of enzymes with substrates is avoided, and the application prospect in the field of enzyme immobilization is promising.

Description

technical field [0001] The invention belongs to the field of enzyme immobilization, and relates to a hydrogel-type enzyme catalytic material containing protease-inorganic hybrid nanoflower synthesized by secondary immobilization, a preparation method and application thereof. Background technique [0002] As an enzyme that catalyzes protein hydrolysis, protease has been widely used in biochemistry, biomedicine, food, textile, chemical industry and other fields. However, since most proteases are easily destroyed under the environmental conditions of strong acid, strong alkali, high temperature and heavy metal ions, the production and application of enzymes are greatly restricted. Therefore, how to improve the stability of enzymes and expand the utilization range of enzymes has become a research hotspot. [0003] In recent years, scholars at home and abroad have made breakthroughs in the synthesis of organic-inorganic hybrid nanoflowers with three-dimensional flower-shaped str...

AI Technical Summary

Problems solved by technology

However, the mechanical strength of this nanoflower-shaped catalytic material is very low, and it is easily affected by the external environment or external forces, resulting in reduced stability, and requires centrifugation to separate the nanoflowers from the substrate, which is not conducive to rapid industrial production.

[0004] At the same time, many domestic and foreign scholars have immobilized enzymes into polymer hydrogels with better biocompatibility, better thermal stability and porous through embedding methods, such as: sodium alginate gel microspheres, graphene Polymer composite hydrogels, chitosan-clay nanocomposite membranes, silicon polymer gel microspheres, etc., have improved the mechanical properties of enzyme-catalyzed materials and improved the stability of enzymes. Unfortunately, after immobilization The diffusion of the enzyme is greatly restricted, and at the same time, a small amount of the enzyme is dissolved from the polymer gel, resulting in a serious loss of enzyme activity

Images