Method and system for removing bisphenol A by utilizing crude enzyme liquid of machilus nanmu

A crude enzyme solution and bisphenol technology, applied in the field of plant biology, can solve the problems of desorption secondary pollution, bisphenol A removal, complex operation, etc., and achieve the effect of easy acquisition, low cost and high catalytic efficiency

Inactive Publication Date: 2018-06-15
HUBEI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] There is no real removal of bisphenol A from the environment. Under certain conditions, desorption will occur and cause secondary pollution; the cost of removing bisphenol A to its standard safe range is high and the operation is complicated

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  • Method and system for removing bisphenol A by utilizing crude enzyme liquid of machilus nanmu
  • Method and system for removing bisphenol A by utilizing crude enzyme liquid of machilus nanmu

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Embodiment Construction

[0026] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0027] The application principle of the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0028] figure 1 , the method for removing bisphenol A by using Runnan crude enzyme liquid provided by the embodiment of the present invention comprises the following steps:

[0029] S101: Preparation of extract: add a certain volume of pure water, add insoluble polyvinylpyrrolidone (PVP-P) to make its concentration 2%, slowly add ammonium sulfate and polyethylene glycol (PEG2000) under stirring, to make its concentration reach 20%, pre-cooled in a 4°C refrigera...

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Abstract

The invention belongs to the technical field of plant biology, and discloses a method and a system for removing bisphenol A by using a crude enzyme liquid of machilus nanmu. The method comprises the following steps of: extracting a crude enzyme liquid: weighing an appropriate amount of mature leaves of machilus nanmu, adding a prepared extracting liquid according to a material-liquid ratio of 1:6,carrying out homogenizing by a high-speed homogenizer for 10 minutes, centrifuging the homogenate at 4 DEG C and at a rate of 5000 r / min for 30 min in a freezing centrifuge, discarding the upper layer liquid, and extracting a lower-phase liquid to obtain the crude enzyme liquid; and removing a buffer liquid, and controlling a reaction system, etc. An enzymatic reaction in the invention has the characteristics of high catalytic efficiency, mild reaction condition, good adjustability and the like; and the removal of bisphenol A with the crude enzyme liquid of machilus nanmu can greatly improvethe removal effect and achieve the purpose of effectively removing environmental pollutants.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and in particular relates to a method and system for removing bisphenol A by using Runnan crude enzyme liquid. Background technique [0002] Bisphenol A, the scientific name is 2,2-bis(4-hydroxyphenyl)ethane, referred to as BPA. Bisphenol A is synthesized from acetone and phenol. Bisphenol A is an important organic chemical raw material, which is widely used in the production of polycarbonate, epoxy resin, flame retardant, plasticizer, rubber anti-aging agent, etc., mainly As a monomer to produce epoxy resin and polycarbonate, the amount of the two accounts for about 95% of the total amount of bisphenol A. Bisphenol A has good mechanical properties, low moisture absorption, and good thermal stability. It is often used in the production of water pipes, food containers, dental products, baby bottles and other products, which are closely related to our lives. With the widespread use of these pro...

Claims

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Application Information

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IPC IPC(8): C02F1/72C12N9/08C02F101/34
CPCC02F1/722C02F1/725C02F2101/345C12N9/0065
Inventor 肖强高艳芳王楠周秀梅张小萌王雨雪
Owner HUBEI UNIV FOR NATITIES
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