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Green separation technique for preparing magnolol and honokiol monomer from mangnolia officinalis raw material

A honokiol and separation technology, which is applied in the field of green separation of magnolol and honokiol monomers, can solve the problems of difficulty in realizing industrialized large-scale production, high production costs, and cumbersome steps, and achieve the solution of solvent Residue, high yield, good safety effect

Inactive Publication Date: 2018-06-15
湖南佳沐生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current method for preparing magnolol and honokiol is to use supercritical extraction or solvent extraction to obtain a crude product containing magnolol and honokiol, and then the crude product is subjected to multiple dissolution extractions, column chromatography, and high-speed countercurrent and other technologies to separate them to obtain high-purity magnolol and a single component of honokiol, but these methods have problems such as cumbersome steps, complicated procedures, solvent residues, and high production costs in the separation process, and it is difficult to realize large-scale industrialization. Production

Method used

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  • Green separation technique for preparing magnolol and honokiol monomer from mangnolia officinalis raw material
  • Green separation technique for preparing magnolol and honokiol monomer from mangnolia officinalis raw material
  • Green separation technique for preparing magnolol and honokiol monomer from mangnolia officinalis raw material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Get 100g of Magnolia officinalis raw material, and described Magnolia officinalis raw material can be Magnolia officinalis leaf powder specifically, detect wherein total phenol content is 14.49% through HPLC, wherein magnolol 7.83%, honokiol 6.66%;

[0025] 2. Magnolia officinalis raw materials were reflux extracted three times with ethanol solution of 7 times the amount, 6 times the amount and 4 times the amount of its mass respectively, the volume concentration of the ethanol solution was 75%, the extraction temperature was 70 ° C, each reflux extraction was 0.5h, combined All the extracts are recovered from ethanol at 35°C and concentrated to 14 degrees Baume. The concentration process can be concentrated by centrifugal film to obtain 20.35g of Magnolia officinalis extract. The total phenol content of Magnolia bark is detected by HPLC 63.75 %, of which magnolol 32.21%, honokiol 31.54%;

[0026] 3. Add 7 times its mass of an alkaline solution with a pH of 10 to the...

Embodiment 2

[0029] 1. Get 100g of Magnolia officinalis raw material, and described Magnolia officinalis raw material can be Magnolia officinalis leaf powder specifically, detect wherein total phenol content is 13.31% through HPLC, wherein magnolol 5.28%, honokiol 8.03%;

[0030] 2. Magnolia officinalis raw materials were reflux extracted three times with ethanol solution of 7 times, 6 times and 4 times of its mass respectively, the volume concentration of the ethanol solution was 85%, the extraction temperature was 80°C, each time refluxed and extracted for 1.5h, combined All the extracts were recovered from ethanol at 40°C and concentrated to 16 degrees Baume. This concentration process was concentrated by centrifugal film to obtain 19.8g of Magnolia officinalis extract. Parkol 23.81%, honokiol 35.53%;

[0031] 3. Add 9 times the mass of an alkaline solution with a pH of 11 to the magnolia bark extract extract, specifically sodium hydroxide solution, stir for 1.5 hours, leave to separate...

Embodiment 3

[0034] 1. Get 100g of Magnolia officinalis raw material, and described Magnolia officinalis raw material can be Magnolia officinalis leaf powder specifically, detect wherein total phenol content is 15.62% through HPLC, wherein magnolol 10.38%, honokiol 5.24%;

[0035] 2. Magnolia officinalis raw materials were reflux extracted three times with the ethanol solution of 7 times the amount, 6 times the amount and 4 times the amount of the mass respectively, the volume concentration of the ethanol solution was 80%, the extraction temperature was 75 ° C, and each time the reflux was extracted for 1 hour, and all were combined. Extract solution, reclaim ethanol under the condition of 38 ℃ and concentrate to 15 degrees Baume, this concentration process adopts centrifuge film to concentrate, obtains Magnolia officinalis extract extractum 22.5g, and HPLC detects that the total phenol content of Magnolia officinalis is 68.32%, wherein Magnolia officinalis phenol 45.79%, honokiol 22.53%; ...

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Abstract

The invention discloses a green separation technique for preparing magnolol and a honokiol monomer from a mangnolia officinalis raw material. The green separation technique comprises the following steps: performing ethanol backflow extraction on a mangnolia officinalis raw material so as to obtain an extract ointment; putting an alkali solution into the mangnolia officinalis extract ointment, leaving to stand to layer, removing an oil layer, and centrifuging so as to obtain precipitate and filtrate; drying the precipitate so as to obtain honokiol; adjusting the filtrate with an acid, leaving to stand, and centrifuging so as to obtain a water insoluble matter, that is, magnolol; dissolving the obtained honokiol with ethanol, recycling the ethanol till a great amount of blocky crystals are generated, and performing cooling crystallization, thereby obtaining high-purity honokiol. Compared with the prior art, the green separation technique is simple and convenient in method, good in energyconsumption, short in preparation cycle, low in cost, free of solvent residue, capable of meeting requirements of environment protection and security, applicable to industrial large-scale production,and extremely great in economic value and social benefit.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine modernization, and in particular relates to a green separation technology for preparing magnolol and honokiol monomers from magnolol raw materials. Background technique [0002] The traditional Chinese medicine Magnolia officinalis is derived from the dry root bark, branch bark or bark of Magnolia officinalis Magnoliaceae deciduous tree plant or Magnolia officinale. The main active ingredients in Magnolia bark are magnolol and honokiol, which have antibacterial and anti-inflammatory effects. With the rapid development of the pharmaceutical industry, the demand for single-component magnolol and honokiol continues to increase. [0003] The current method for preparing magnolol and honokiol is to use supercritical extraction or solvent extraction to obtain a crude product containing magnolol and honokiol, and then the crude product is subjected to multiple dissolution extractions, column c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C39/21C07C37/68C07C37/72C07C37/84
CPCC07C37/685C07C37/72C07C37/84C07C39/21
Inventor 李海黄民定刘兴科
Owner 湖南佳沐生物科技有限公司
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