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Method for evaluating efficacy of sunscreen product

An evaluation method and functional technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as health damage, human body testing method ethics and moral scruples, etc., to shorten the cycle, speed up the evaluation speed, and simplify the operation.

Inactive Publication Date: 2018-06-15
广东省保化检测中心有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the ultraviolet radiation produced by various UVA and UVB light sources has unknown consequences on human health, the human body test method has great ethical and moral scruples

Method used

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  • Method for evaluating efficacy of sunscreen product
  • Method for evaluating efficacy of sunscreen product
  • Method for evaluating efficacy of sunscreen product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The present embodiment provides a method for evaluating the efficacy of sunscreen products, comprising the following steps,

[0039] (1) Obtain culture fluid, add sunscreen product to be tested and rhamnolipid in described culture fluid, obtain sample solution, the active ingredient of described sunscreen product to be tested is crocodile oil, and the concentration of sunscreen product to be tested is 0.5mg / ml, the mass ratio of sunscreen product to be tested to rhamnolipid is 50:1.

[0040] The components of the culture solution include: sodium chloride, potassium chloride, calcium chloride, magnesium sulfate, methylene blue and water.

[0041] (2) Cultivate zebrafish larvae in the sample solution, the time of the cultivation is T, and within the time T, carry out ultraviolet irradiation to the zebrafish larvae;

[0042] The method for obtaining zebrafish larvae is as follows: sexually mature zebrafish (the AB line, introduced by the National Zebrafish Resource Cente...

Embodiment 2

[0061] The present embodiment provides a method for evaluating the efficacy of sunscreen products, comprising the following steps,

[0062] (1) Obtain culture fluid, add sunscreen product to be tested and rhamnolipid in described culture fluid, obtain sample solution, the active ingredient of described sunscreen product to be tested is crocodile oil, the concentration of sunscreen product to be tested is 1mg / ml, the mass ratio of sunscreen product to be tested to rhamnolipid is 50:1.

[0063] The components of the culture solution include: sodium chloride, potassium chloride, calcium chloride, magnesium sulfate, methylene blue and water.

[0064] (2) Cultivate zebrafish larvae in the sample solution, the time of the cultivation is T, and within the time T, carry out ultraviolet irradiation to the zebrafish larvae;

[0065] Sexually mature zebrafish (line AB, introduced by the National Zebrafish Resource Center and multiplied in this laboratory) were separated into male and f...

Embodiment 3

[0072] The present embodiment provides a method for evaluating the efficacy of sunscreen products, comprising the following steps,

[0073] (1) Obtain culture fluid, add sunscreen product to be tested and rhamnolipid in described culture fluid, obtain sample solution, the active ingredient of described sunscreen product to be tested is crocodile oil, the concentration of sunscreen product to be tested is 2mg / ml, the mass ratio of sunscreen product to be tested to rhamnolipid is 50:1.

[0074] The components of the culture solution include: sodium chloride, potassium chloride, calcium chloride, magnesium sulfate, methylene blue and water.

[0075] (2) Cultivate zebrafish larvae in the sample solution, the time of the cultivation is T, and within the time T, carry out ultraviolet irradiation to the zebrafish larvae;

[0076] Sexually mature zebrafish (line AB, introduced by the National Zebrafish Resource Center and multiplied in this laboratory) were separated into male and f...

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Abstract

The invention relates to a method for evaluating efficacy of a sunscreen product. The method comprises the following steps: obtaining a culture solution, adding the to-be-tested sunscreen product withan active ingredient of alligator oil and a biological surfactant into the culture solution to obtain a sample solution; culturing zebrafish juveniles in the sample solution for culture time of T, and during the time of T, exposing the zebrafish juveniles to ultraviolet radiation; transferring the zebrafish juveniles exposed to the ultraviolet radiation to the culture solution for culture, and recording the degree of damage of pelvic fins, caudal fins, and dorsal fins of the zebrafish juveniles. According to the method for evaluating the efficacy of the sunscreen product, zebrafish is used asa model organism to evaluate the efficacy of the sunscreen product with the active ingredient of the alligator oil, and damage to the human health caused by the ultraviolet radiation dose of testingmethods separated from biological organisms and human bodies can be avoided.

Description

technical field [0001] The invention relates to the field of cosmetics, in particular to a method for evaluating the efficacy of sunscreen cosmetics. Background technique [0002] With the improvement of people's material living standards and changes in life concepts, people's travel and leisure activities increase, so that the skin is exposed to ultraviolet rays for an increasing time, and the destruction of the ozone layer in the atmosphere is intensified, and more and more ultraviolet rays are radiated to human skin. . Therefore, the best-selling sunscreen cosmetics on the market, the active ingredients in sunscreen cosmetics are sunscreen agents, including physical blocking agents and chemical absorbers. Physical sunscreens are usually opaque substances that reflect and scatter all ultraviolet and visible light, while chemical absorbers act to selectively absorb ultraviolet light. Cosmetics added with sunscreens are applied on the skin to form a protective film, which ...

Claims

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Application Information

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IPC IPC(8): G01N33/00
CPCG01N33/00
Inventor 郑伟东
Owner 广东省保化检测中心有限公司
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