Hydrogen-production composite biological agent
A technology of biological preparation and compound bacterial liquid, applied in the biological field, can solve the problem of low hydrogen production efficiency of algae, and achieve the effect of good hydrogen production effect, promotion of hydrogen production, and good hydrogen production capacity.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0033] A hydrogen-producing composite biological agent, which includes Chlorella pyrenoidosa, Enterococcus faecium and Rhodopseudomonas palustris.
[0034] Specifically, the composite biological preparation is prepared according to the following steps:
[0035] Step 1) Preparation of seed solution: pick Chlorella pyrenoidosa into a culture bottle filled with 1L of proliferation medium, and the light intensity is 150 μmol m -2 ·s -1 , cultivated at 26°C, shaking the Erlenmeyer flask 2-3 times a day; cultivated for 4 days to obtain seed liquid in the logarithmic growth phase; the proliferation medium contained the following components per liter: glucose 3g, yeast powder 1g, sodium nitrate 1g, Sodium sulfite 1g, sodium chloride 0.5g, potassium dihydrogen phosphate 0.5g, magnesium sulfate 0.2g, ferrous sulfate 0.1g;
[0036] Step 2) Preparation of algae solution: inoculate the seed solution of step 1) into the TAP culture solution at an inoculation amount of 6% for cultivation, ...
Embodiment 2
[0042] A hydrogen-producing composite biological agent, which includes Chlorella pyrenoidosa, Enterococcus faecium and Rhodopseudomonas palustris.
[0043] Specifically, the composite biological preparation is prepared according to the following steps:
[0044] Step 1) Preparation of seed liquid: pick Chlorella pyrenoidosa into a culture bottle filled with 1L of proliferation medium, and the light intensity is 150 μmol m -2 ·s -1 , cultivated at 26°C, shaking the Erlenmeyer flask 2-3 times a day; cultivated for 3 days to obtain seed liquid in the logarithmic growth phase; the proliferation medium contained the following components per liter: glucose 3g, yeast powder 1g, sodium nitrate 1g, Sodium sulfite 1g, sodium chloride 0.5g, potassium dihydrogen phosphate 0.5g, magnesium sulfate 0.2g, ferrous sulfate 0.1g;
[0045] Step 2) Preparation of algae solution: inoculate the seed solution of step 1) into TAP culture solution at an inoculum amount of 8% for cultivation, and contr...
Embodiment 3
[0051] Hydrogen production performance test of the composite biological preparation of the present invention:
[0052] Set the total volume of the reactor to be 10L, wherein the volume of the liquid is 8L, and the space above the liquid is 2L. The amount of gas produced during the reaction is obtained by multiplying the gas composition by the volume of the gas. Perform gas chromatography on the gas phase product inside the reactor Determination;
[0053] Taking Example 1 as an example, a control group is set at the same time, wherein, control group 1: only adopts Enterococcus faecium, does not add Rhodopseudomonas palustris, and the rest is the same as Example 1; Control group 2: only adopts Rhodopseudomonas palustris Bacteria, do not add Enterococcus faecium, the rest are the same as in Example 1; Control group 3: do not add composite bacteria liquid, the rest are the same as in Example 1;
[0054] The hydrogen production and hydrogen content of Example 1 and Comparative Exa...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More