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Method capable of producing fermentable sugars from corncob residues by efficient enzymatic hydrolysis

A technology of corn cob and enzymatic hydrolysis, applied in the direction of fermentation, etc., can solve the problems of undiscovered patent publications, etc., and achieve the effect of improving the efficiency of enzymatic hydrolysis, reducing the amount of use, and reducing input costs

Inactive Publication Date: 2018-06-19
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Through the search, no patent publications related to the patent application of the present invention have been found

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A method for producing fermentable sugars through efficient enzymatic hydrolysis of corncob residues, the steps are as follows:

[0031] [1] Slurry preparation: Air-dry and pulverize the corncob residue, screen with a 40-mesh sieve to obtain the treated corncob residue, and put the obtained treated corncob residue into a sealed bag for subsequent use;

[0032] [2] Pretreatment by chemical method: Weigh the above-mentioned corn cob residue of a certain absolute dry mass, put it into the reactor, add sodium sulfite solid, then add distilled water to control the mass concentration of the raw material suspension to be 15.0%, stir to make Mix the raw materials and the liquid medicine evenly, adjust the pH of the reaction system to 2.0 with 0.1mol / L NaOH solution, and control the reaction temperature to 80°C;

[0033] Wherein, the consumption of sodium sulfite solid is 1.0% (mass fraction) of the dry mass of corn cob residue raw material;

[0034] [3] After the reaction reache...

Embodiment 2

[0042] A method capable of realizing high-efficiency enzymatic hydrolysis of corn cob residue to produce fermentable sugar, the steps are as follows:

[0043] [1] Slurry preparation: Air-dry and pulverize the corncob residue, screen with a 40-mesh sieve to obtain the treated corncob residue, and put the obtained treated corncob residue into a sealed bag for subsequent use;

[0044] [2] Pretreatment by chemical method: take a certain absolute dry mass of the above-mentioned treated corn cob residue raw material, put it into the reactor, add sodium sulfite solid, then add distilled water to control the mass concentration of the raw material suspension to 15.0%, stir to Mix the raw materials and the liquid medicine evenly, adjust the pH of the reaction system to 3.0 with 0.1mol / L NaOH solution, and control the reaction temperature at 80°C;

[0045] Wherein, the consumption of sodium sulfite solid is 2.0% (mass fraction) of the dry mass of corncob residue raw material;

[0046] [...

Embodiment 3

[0054] A method for producing fermentable sugars through efficient enzymatic hydrolysis of corncob residues, the steps are as follows:

[0055] [1] Slurry preparation: Air-dry and pulverize the corncob residue, screen with a 40-mesh sieve, and put the obtained raw material into a sealed bag for subsequent use;

[0056] [2] Pretreatment by chemical method: Weigh the above-mentioned corn cob residue of a certain absolute dry mass, put it into the reactor, add sodium sulfite solid, then add distilled water to control the mass concentration of the raw material suspension to be 15.0%, stir to make Mix the raw materials and the liquid medicine evenly, adjust the pH of the reaction system to 4.0 with 0.1mol / L NaOH solution, and control the reaction temperature at 80°C;

[0057] Wherein, the consumption of sodium sulfite solid is 4.0% (mass fraction) of absolute dry mass of corn cob residue raw material;

[0058] [3] After the reaction reaches 60 minutes, collect the corncob residue ...

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Abstract

The invention discloses a method capable of producing fermentable sugars from corncob residues by efficient enzymatic hydrolysis. The method comprises the following steps: (1) preparing raw materials;(2) performing pretreatment with a chemical method; (3) collecting reacted corncob residues, repeatedly washing the corncob residues multiple times until the corncob residues are neutral to obtain washed corncob residues; (4) performing enzymatic hydrolysis to obtain a hydrolysate for completion of production of the fermentable sugars from the corncob residues by efficient enzymatic hydrolysis. According to the method, the corncob residues are treated with a chemical pretreatment technique, hydrophilic performance of lignin in fiber raw materials is improved, the lignin is dissolved out easily, and accordingly, enzymatic hydrolysis yield of the corncob residues is increased; the corncob residues can be used in a higher-value manner with the method, investing cost for enzymatic hydrolysisis reduced, enzymatic hydrolysis efficiency is increased, raw material basis is provided for follow-up processing and utilization or production of products with high added value, so that application field and range of the corncob residues are expanded, and use quantity of wood fiber raw materials can be reduced effectively.

Description

technical field [0001] The invention belongs to the technical field of combining high-efficiency utilization of biomass resources and agricultural waste, in particular a method capable of realizing high-efficiency enzymatic hydrolysis of corncob residues to produce fermentable sugar. technical background [0002] With the continuous development of the economy, people's demand for energy is increasing day by day, especially the dependence on fossil energy such as coal, oil, and natural gas is increasing. The energy crisis has become one of the major challenges facing human society. At the same time, due to the massive combustion of fossil fuels, a series of environmental problems such as air pollution, acid rain, greenhouse effect and ozone layer destruction are becoming more and more serious. Therefore, under the dual challenges of energy crisis and environmental pollution, it is particularly important to seek a new, renewable and clean energy to replace fossil energy. [0...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/02C12P19/14
CPCC12P19/02C12P19/14C12P2201/00
Inventor 刘苇汪兵侯庆喜王思
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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