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Flaxseed meal anti-oxidation hexapeptide as well as preparation method and application thereof

A flaxseed meal and antioxidant technology, applied in the biological field, can solve problems such as waste of high-quality protein resources, and achieve the effect of strong scavenging effect

Inactive Publication Date: 2018-06-22
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, flaxseed gum and lignans are the focus of research on flaxseed meal, but the research on the preparation of active peptides by enzymatic hydrolysis of flaxseed meal rarely involves
In addition, the deoiled linseed meal is mostly used for animal feed, or treated as waste, resulting in a great waste of high-quality protein resources

Method used

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  • Flaxseed meal anti-oxidation hexapeptide as well as preparation method and application thereof
  • Flaxseed meal anti-oxidation hexapeptide as well as preparation method and application thereof
  • Flaxseed meal anti-oxidation hexapeptide as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0019] The separation and purification of the antioxidant heptapeptide of the present invention includes two steps of Sephadex G-25 gel filtration chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC).

[0020] Preparation of antioxidative heptapeptide hydrolyzate of linseed meal: use trypsin to hydrolyze it under optimal conditions: substrate concentration 2% (w / v), pH 8.50, enzyme addition 4000 U / g, temperature 37 ℃ , hydrolyze the enzyme for 3 h, inactivate it in a boiling water bath for 10 min, centrifuge at 12000 rpm for 20 min, take the supernatant and freeze-dry it, which is the protein hydrolyzate of linseed meal.

[0021] Sephadex G-25 Gel Filtration Chromatography: The lyophilized powder of linseed meal protein enzymatic hydrolyzate was dissolved in deionized water, and centrifuged at 12000 rpm for 15 min. The supernatant was filtered through a 0.22 μm pore size microfiltration membrane. The Sephadex G-25 gel column (1.6cm×100cm) was equ...

Embodiment 2

[0026] The natural antioxidant hexapeptide activity obtained in Example 1 is studied:

[0027] ABTS free radical scavenging ability: prepare 7 mmol / L ABTS with distilled water + solution and 2.45 mmol / L potassium persulfate solution (must be placed at room temperature for 16 h before use), ABTS + Mix with potassium persulfate solution at a volume ratio of 1:1, and dilute the mixture to the absorbance value A with pH 7.4, 5 mmol / L phosphate buffer solution before use 734 is 0.70±0.02. Take 0.5 mL of samples with different concentrations and 0.5 mL of ABTS radical solution, mix and let stand for 10 min, then measure the absorbance at 734 nm. Deionized water and reduced glutathione were used instead of samples as blank control and positive control, respectively. ABTS free radical scavenging activity is calculated according to the following formula (1):

[0028] (2)

[0029] In the formula, A 0 : Absorbance value of blank control group; A s : Absorbance value of the samp...

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Abstract

The invention discloses flaxseed meal anti-oxidation hexapeptide as well as a preparation method and application thereof. The sequence of the anti-oxidation hexapeptide is Tyr-Ala-Pro-Phe-Tyr-Leu (YAPFYL); and in vitro experiments show that the polypeptide has good reducing capacity and also can effectively scavenge ABTS and various reactive oxygen species (ORAC). The anti-oxidation peptide disclosed by the invention has the characteristics of simple structure, high anti-oxidation activity and the like, can serve as a good substitute for the existing artificially-synthesized antioxidant, and has important values for development and application of novel anti-oxidation health-care products.

Description

technical field [0001] The invention relates to an antioxidant hexapeptide of linseed meal and its preparation method and application, belonging to the field of biotechnology. Background technique [0002] Oxidation is the main cause of various diseases and various chronic diseases. The surge of free radicals in the body induces oxidative stress, and the body's antioxidant defense system is seriously unbalanced, resulting in cell damage. Oxidation of biomolecules plays a crucial role in all living organisms, and it is a free radical-mediated process in which free radicals attack biomacromolecules such as proteins, nucleic acids, and lipids, resulting in lipid peroxidation and anti-inflammatory effects. Oxidase activity is reduced. The free radicals induced by exogenous chemical substances or mitochondrial respiration, such as superoxide anion, hydroxyl radicals and active oxygen radicals, are very unstable and react rapidly with other groups or substances in the body, resul...

Claims

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Application Information

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IPC IPC(8): C07K7/06C12P21/06C07K1/16C07K1/20C07K1/36A23L33/18
CPCA23L33/18A23V2002/00C07K7/06C12P21/06A23V2200/30A23V2250/55
Inventor 洪晶王惠敏蔡甜甜孟春
Owner FUZHOU UNIV
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